Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Mechanisms of anandamide‐induced vasorelaxation in rat isolated coronary arteries

Mechanisms of anandamide‐induced vasorelaxation in rat isolated coronary arteries The cannabinoid arachidonyl ethanolamide (anandamide) caused concentration‐dependent relaxation of 5‐HT‐precontracted, myograph‐mounted, segments of rat left anterior descending coronary artery. This relaxation was endothelium‐independent, unaffected by the fatty acid amide hydrolase inhibitor, arachidonyl trifluoromethyl ketone (10 μM), and mimicked by the non‐hydrolysable anandamide derivative, methanandamide. Relaxations to anandamide were attenuated by the cannabinoid receptor antagonist, SR 141716A (3 μM), but unaffected by AM 251 (1 μM) and AM 630 (1 μM), more selective antagonists of cannabinoid CB1 and CB2 receptors respectively. Palmitoylethanolamide, a selective CB2 receptor agonist, did not relax precontracted coronary arteries. Anandamide relaxations were not affected by inhibition of sensory nerve transmission with capsaicin (10 μM) or blockade of vanilloid VR1 receptors with capsazepine (5 μM). Nevertheless capsaicin relaxed coronary arteries in a concentration‐dependent and capsazepine‐sensitive manner, confirming functional sensory nerves were present. In contrast, capsazepine and capsaicin did inhibit anandamide relaxations in methoxamine‐precontracted rat small mesenteric arteries. Relaxations to anandamide were inhibited by TEA (1 mM) or iberiotoxin (50 nM), blockers of large conductance, Ca2+‐activated K+ channels (BKCa). Gap junction inhibition with 18α‐glycyrrhetinic acid (100 μM) did not affect anandamide relaxations. This study shows anandamide relaxes the rat coronary artery by a novel mechanism. Anandamide‐induced relaxations do not involve the endothelium, degradation into active metabolites, or activation of cannabinoid CB1 or CB2 receptors, but may involve activation of BKCa. Vanilloid receptor activation also has no role in the effects of anandamide in coronary arteries, even though functional sensory nerves are present. British Journal of Pharmacology (2001) 134, 921–929; doi:10.1038/sj.bjp.0704333 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png British Journal of Pharmacology Wiley

Mechanisms of anandamide‐induced vasorelaxation in rat isolated coronary arteries

Download PDF
9 pages

Loading next page...
 
/lp/wiley/mechanisms-of-anandamide-induced-vasorelaxation-in-rat-isolated-MAOgzimYsq

References (36)

Publisher
Wiley
Copyright
2001 British Pharmacological Society
ISSN
0007-1188
eISSN
1476-5381
DOI
10.1038/sj.bjp.0704333
pmid
11606334
Publisher site
See Article on Publisher Site

Abstract

The cannabinoid arachidonyl ethanolamide (anandamide) caused concentration‐dependent relaxation of 5‐HT‐precontracted, myograph‐mounted, segments of rat left anterior descending coronary artery. This relaxation was endothelium‐independent, unaffected by the fatty acid amide hydrolase inhibitor, arachidonyl trifluoromethyl ketone (10 μM), and mimicked by the non‐hydrolysable anandamide derivative, methanandamide. Relaxations to anandamide were attenuated by the cannabinoid receptor antagonist, SR 141716A (3 μM), but unaffected by AM 251 (1 μM) and AM 630 (1 μM), more selective antagonists of cannabinoid CB1 and CB2 receptors respectively. Palmitoylethanolamide, a selective CB2 receptor agonist, did not relax precontracted coronary arteries. Anandamide relaxations were not affected by inhibition of sensory nerve transmission with capsaicin (10 μM) or blockade of vanilloid VR1 receptors with capsazepine (5 μM). Nevertheless capsaicin relaxed coronary arteries in a concentration‐dependent and capsazepine‐sensitive manner, confirming functional sensory nerves were present. In contrast, capsazepine and capsaicin did inhibit anandamide relaxations in methoxamine‐precontracted rat small mesenteric arteries. Relaxations to anandamide were inhibited by TEA (1 mM) or iberiotoxin (50 nM), blockers of large conductance, Ca2+‐activated K+ channels (BKCa). Gap junction inhibition with 18α‐glycyrrhetinic acid (100 μM) did not affect anandamide relaxations. This study shows anandamide relaxes the rat coronary artery by a novel mechanism. Anandamide‐induced relaxations do not involve the endothelium, degradation into active metabolites, or activation of cannabinoid CB1 or CB2 receptors, but may involve activation of BKCa. Vanilloid receptor activation also has no role in the effects of anandamide in coronary arteries, even though functional sensory nerves are present. British Journal of Pharmacology (2001) 134, 921–929; doi:10.1038/sj.bjp.0704333

Journal

British Journal of Pharmacology – Wiley

Published: Oct 1, 2001

There are no references for this article.