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Extraction of extracellular polymeric substances from the photosynthetic bacterium Rhodopseudomonas acidophila

Extraction of extracellular polymeric substances from the photosynthetic bacterium... Among the four methods for extracting extracellular polymeric substances (EPS) from Rhodopseudomonas acidophila (EDTA, NaOH, H2SO4, heating/centrifugation), EDTA extraction was found to be the most effective. The contents of the major components of EPS from R. acidophila, i.e., carbohydrate, protein and nucleic acid, were 6.5, 58.4 and 5.4 mg g−1 dry cells, respectively. The optimum extraction time was 1–3 h and the EDTA dosage was approximately 2.8 g g−1 dry cells. Under these conditions, no cell lysis was observed. The EPS content and the percentage of the three main components were greatly dependent on the extraction method. The intensity of absorption peaks for photosynthetic pigments in the UV–visible spectrum of bacteria remained unchanged prior to and after EDTA extraction; and no pigment peaks appeared in the EPS spectrum. This suggests that few cells were destroyed and lysis did not occur. UV–visible spectrum analysis, an easy and rapid technique, could be used to monitor cell lysis during EPS extraction from R. acidophila. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Microbiology and Biotechnology Springer Journals

Extraction of extracellular polymeric substances from the photosynthetic bacterium Rhodopseudomonas acidophila

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References (25)

Publisher
Springer Journals
Copyright
Copyright © 2004 by Springer-Verlag
Subject
LifeSciences
ISSN
0175-7598
eISSN
1432-0614
DOI
10.1007/s00253-004-1704-5
pmid
15309338
Publisher site
See Article on Publisher Site

Abstract

Among the four methods for extracting extracellular polymeric substances (EPS) from Rhodopseudomonas acidophila (EDTA, NaOH, H2SO4, heating/centrifugation), EDTA extraction was found to be the most effective. The contents of the major components of EPS from R. acidophila, i.e., carbohydrate, protein and nucleic acid, were 6.5, 58.4 and 5.4 mg g−1 dry cells, respectively. The optimum extraction time was 1–3 h and the EDTA dosage was approximately 2.8 g g−1 dry cells. Under these conditions, no cell lysis was observed. The EPS content and the percentage of the three main components were greatly dependent on the extraction method. The intensity of absorption peaks for photosynthetic pigments in the UV–visible spectrum of bacteria remained unchanged prior to and after EDTA extraction; and no pigment peaks appeared in the EPS spectrum. This suggests that few cells were destroyed and lysis did not occur. UV–visible spectrum analysis, an easy and rapid technique, could be used to monitor cell lysis during EPS extraction from R. acidophila.

Journal

Applied Microbiology and Biotechnology – Springer Journals

Published: Aug 12, 2004

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