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Nitric oxide retards xanthine oxidase-mediated superoxide anion generation in Phalaenopsis flower: an implication of NO in the senescence and oxidative stress regulation

Nitric oxide retards xanthine oxidase-mediated superoxide anion generation in Phalaenopsis... Senescence is a developmentally regulated and highly ordered sequence of events. Senescence leads to abscission of plant organs and eventually leads to death of a plant or part of it. Present study revealed that Phalaenopsis flower undergo senescence due to over activation of O 2 ·− generating xanthine oxidase (XO), which consequently increases the concentrations of O 2 ·− leading to enhanced oxidative damage and disturbed cellular redox environment as indicated by increased lipid peroxidation and DHA/AsA + DHA ratio, respectively. While activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), and non-specific peroxidase (POD) were enhanced in sepals and petals of old flower, activities of catalase (CAT) and glutathione reductase (GR) were decreased. Exogenous application of nitric oxide (NO) retarded H 2 O 2 -induced senescence of Phalaenopsis flower by downregulating activity of XO and concentrations of O 2 ·− , H 2 O 2 and malondialdehyde (MDA, an index of lipid peroxidation). Exogenous application of NO also downregulated SOD activity and upregulated antioxidant enzymes involved in the detoxification of H 2 O 2 (CAT and APX), and in the regulation of redox couples viz, monodehydroascorbate reductase (MDHAR) and GR, together with the modulation in non-protein thiol status and DHA/AsA + DHA ratio. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

Nitric oxide retards xanthine oxidase-mediated superoxide anion generation in Phalaenopsis flower: an implication of NO in the senescence and oxidative stress regulation

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References (49)

Publisher
Springer Journals
Copyright
Copyright © 2009 by Springer-Verlag
Subject
Life Sciences; Plant Biochemistry; Biotechnology; Plant Sciences ; Cell Biology
ISSN
0721-7714
eISSN
1432-203X
DOI
10.1007/s00299-008-0632-8
pmid
18985352
Publisher site
See Article on Publisher Site

Abstract

Senescence is a developmentally regulated and highly ordered sequence of events. Senescence leads to abscission of plant organs and eventually leads to death of a plant or part of it. Present study revealed that Phalaenopsis flower undergo senescence due to over activation of O 2 ·− generating xanthine oxidase (XO), which consequently increases the concentrations of O 2 ·− leading to enhanced oxidative damage and disturbed cellular redox environment as indicated by increased lipid peroxidation and DHA/AsA + DHA ratio, respectively. While activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), and non-specific peroxidase (POD) were enhanced in sepals and petals of old flower, activities of catalase (CAT) and glutathione reductase (GR) were decreased. Exogenous application of nitric oxide (NO) retarded H 2 O 2 -induced senescence of Phalaenopsis flower by downregulating activity of XO and concentrations of O 2 ·− , H 2 O 2 and malondialdehyde (MDA, an index of lipid peroxidation). Exogenous application of NO also downregulated SOD activity and upregulated antioxidant enzymes involved in the detoxification of H 2 O 2 (CAT and APX), and in the regulation of redox couples viz, monodehydroascorbate reductase (MDHAR) and GR, together with the modulation in non-protein thiol status and DHA/AsA + DHA ratio.

Journal

Plant Cell Reports – Springer Journals

Published: Feb 1, 2009

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