Access the full text.
Sign up today, get DeepDyve free for 14 days.
C. Karmazyn-Campelli, L. Fluss, T. Leighton, P. Stragier (1992)
The spoIIN279(ts) mutation affects the FtsA protein of Bacillus subtilis.Biochimie, 74 7-8
Elke Menkel, G. Thierbach, L. Eggeling, Hermann Sahm (1989)
Influence of increased aspartate availability on lysine formation by a recombinant strain of Corynebacterium glutamicum and utilization of fumarateApplied and Environmental Microbiology, 55
Iris Eggeling, C. Cordes, L. Eggeling, H. Sahm (2004)
Regulation of acetohydroxy acid synthase in Corynebacterium glutamicum during fermentation of α-ketobutyrate to l-isoleucineApplied Microbiology and Biotechnology, 25
Maria Renna, Nazalan, Najimudin, Leslie Winik, S. Zahler (1993)
Regulation of the Bacillus subtilis alsS, alsD, and alsR genes involved in post-exponential-phase production of acetoinJournal of Bacteriology, 175
M. Gordillo, A. Sanz, Antoni Sánchez, F. Valero, J. Montesinos, J. Lafuente, C. Solà (1998)
Enhancement of Candida rugosa lipase production by using different control fed-batch operational strategies.Biotechnology and bioengineering, 60 2
Effendi Leonard, Weerawat Runguphan, S. O’Connor, K. Prather (2009)
Opportunities in metabolic engineering to facilitate scalable alkaloid production.Nature chemical biology, 5 5
Sui-Lam Wong (1995)
Advances in the use of Bacillus subtilis for the expression and secretion of heterologous proteins.Current opinion in biotechnology, 6 5
D. Nielsen, Effendi Leonard, Sang‐Hwal Yoon, Hsien-Chung Tseng, Clara Yuan, K. Prather (2009)
Engineering alternative butanol production platforms in heterologous bacteria.Metabolic engineering, 11 4-5
J. Marles-Wright, Tim Grant, O. Delumeau, Gijs Duinen, S. Firbank, P. Lewis, J. Murray, J. Newman, Maureen Quin, P. Race, A. Rohou, W. Tichelaar, M. Heel, R. Lewis (2008)
Molecular Architecture of the "Stressosome," a Signal Integration and Transduction HubScience, 322
Yingbo Zhu, Xun Chen, Tao Chen, Xue-Ming Zhao (2007)
Enhancement of riboflavin production by overexpression of acetolactate synthase in a pta mutant of Bacillus subtilis.FEMS microbiology letters, 266 2
Damien Leyval, D. Uy, S. Delaunay, J. Goergen, J. Engasser (2003)
Characterisation of the enzyme activities involved in the valine biosynthetic pathway in a valine-producing strain of Corynebacterium glutamicum.Journal of biotechnology, 104 1-3
Yunxia Duan, Tao Chen, Xun Chen, Xue-Ming Zhao (2010)
Overexpression of glucose-6-phosphate dehydrogenase enhances riboflavin production in Bacillus subtilisApplied Microbiology and Biotechnology, 85
E. Hayhurst, L. Kailas, J. Hobbs, S. Foster (2008)
Cell wall peptidoglycan architecture in Bacillus subtilisProceedings of the National Academy of Sciences, 105
P. Unrean, C. Trinh, F. Srienc (2010)
Rational design and construction of an efficient E. coli for production of diapolycopendioic acid.Metabolic engineering, 12 2
S Atsumi (2010)
651Appl Microbiol Biotechnol, 85
A. Petersohn, Matthias Brigulla, S. Haas, J. Hoheisel, U. Völker, M. Hecker (2001)
Global Analysis of the General Stress Response ofBacillus subtilisJournal of Bacteriology, 183
W. Holtzclaw, L. Chapman (1975)
Degradative acetolactate synthase of Bacillus subtilis: purification and propertiesJournal of Bacteriology, 121
C. Bi, Xueli Zhang, L. Ingram, J. Preston (2009)
Genetic Engineering of Enterobacter asburiae Strain JDR-1 for Efficient Production of Ethanol from Hemicellulose HydrolysatesApplied and Environmental Microbiology, 75
P. Haima, S. Bron, G. Venemâ (1987)
The effect of restriction on shotgun cloning and plasmid stability in Bacillus subtilis MarburgMolecular and General Genetics MGG, 209
S. Atsumi, Tung-Yun Wu, Eva-Maria Eckl, Sarah Hawkins, T. Buelter, J. Liao
Applied Genetics and Molecular Biotechnology
Kevin Smith, K. Cho, J. Liao (2010)
Engineering Corynebacterium glutamicum for isobutanol productionApplied Microbiology and Biotechnology, 87
Murat Karabektaş, M. Hosoz (2009)
Performance and emission characteristics of a diesel engine using isobutanol–diesel fuel blendsRenewable Energy, 34
Antje Chang, Maurice Scheer, A. Grote, I. Schomburg, D. Schomburg (2008)
BRENDA, AMENDA and FRENDA the enzyme information system: new content and tools in 2009Nucleic Acids Research, 37
S Atsumi, T-Y Wu, E-M Eckl, SD Hawkins, T Buelter, JC Liao (2010)
Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/alcohol dehydrogenase genesAppl Microbiol Biotechnol, 85
EJ Steen, R Chan, N Prasad, S Myers, CJ Petzold, A Redding, M Ouellet, JD Keasling (2008)
Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanolMicrob Cell Fact, 7
V. Martin, D. Pitera, Sydnor Withers, J. Newman, J. Keasling (2003)
Engineering a mevalonate pathway in Escherichia coli for production of terpenoidsNature Biotechnology, 21
K. Livak, Thomas Schmittgen (2001)
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.Methods, 25 4
C. Carlini, A. Macinai, M. Marchionna, M. Noviello, A. Galletti, G. Sbrana (2003)
Selective synthesis of isobutanol by means of the Guerbet reaction: Part 3: Methanol/n-propanol condensation by using bifunctional catalytic systems based on nickel, rhodium and ruthenium species with basic componentsJournal of Molecular Catalysis A-chemical, 206
Kechun Zhang, M. Sawaya, D. Eisenberg, J. Liao (2008)
Expanding metabolism for biosynthesis of nonnatural alcoholsProceedings of the National Academy of Sciences, 105
K. Schleifer, J. Kraus, C. Dvorak, R. Kilpper-Bälz, M. Collins, W. Fischer (1985)
Transfer of Streptococcus lactis and related streptococci to the genus Lactococcus gen. nov.Systematic and Applied Microbiology, 6
S. Romero‐Garcia, Claudia Hernández-Bustos, E. Merino, G. Gosset, Alfredo Martínez (2009)
Homolactic fermentation from glucose and cellobiose using Bacillus subtilisMicrobial Cell Factories, 8
Jette Thykaer, J. Nielsen, W. Wohlleben, T. Weber, Michael Gutknecht, A. Lantz, E. Stegmann (2010)
Increased glycopeptide production after overexpression of shikimate pathway genes being part of the balhimycin biosynthetic gene cluster.Metabolic engineering, 12 5
Xiaojun Ji, He Huang, Jiangyu Zhu, Lu-jing Ren, Zhi-Kui Nie, Jun Du, Shuang Li (2010)
Engineering Klebsiella oxytoca for efficient 2, 3-butanediol production through insertional inactivation of acetaldehyde dehydrogenase geneApplied Microbiology and Biotechnology, 85
Eric Steen, Rossana Chan, Nilu Prasad, Samuel Myers, C. Petzold, Alyssa Redding, M. Ouellet, J. Keasling, Samuel, Alyssa Gov, Mario Gov, Jay Gov, Keasling
Microbial Cell Factories Metabolic Engineering of Saccharomyces Cerevisiae for the Production of N-butanol
Oliver Hädicke, Steffen Klamt (2010)
CASOP: a computational approach for strain optimization aiming at high productivity.Journal of biotechnology, 147 2
Michael Connor, J. Liao (2008)
Engineering of an Escherichia coli Strain for the Production of 3-Methyl-1-ButanolApplied and Environmental Microbiology, 74
S. Atsumi, T. Hanai, J. Liao (2008)
Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuelsNature, 451
J. Sambrook, E. Fritsch, T. Maniatis (2001)
Molecular Cloning: A Laboratory Manual
Xiaojun Ji, He Huang, Shuang Li, Jun Du, Min Lian (2008)
Enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytocaBiotechnology Letters, 30
M. Etschmann, J. Schrader (2006)
An aqueous–organic two-phase bioprocess for efficient production of the natural aroma chemicals 2-phenylethanol and 2-phenylethylacetate with yeastApplied Microbiology and Biotechnology, 71
Adam Feist, Daniel Zielinski, Jeffrey Orth, Jan Schellenberger, Markus Herrgård, B. Palsson (2010)
Model-driven evaluation of the production potential for growth-coupled products of Escherichia coli.Metabolic engineering, 12 3
M. Inui, Masako Suda, S. Kimura, K. Yasuda, Hiroaki Suzuki, H. Toda, Shogo Yamamoto, S. Okino, N. Suzuki, H. Yukawa (2008)
Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coliApplied Microbiology and Biotechnology, 77
Ana Brochado, C. Matos, B. Møller, J. Hansen, U. Mortensen, K. Patil (2010)
Improved vanillin production in baker's yeast through in silico designMicrobial Cell Factories, 9
E. Garvie (1980)
Bacterial lactate dehydrogenases.Microbiological reviews, 44 1
M. Plaza, P. Palencia, C. Peláez, T. Requena (2004)
Biochemical and molecular characterization of alpha-ketoisovalerate decarboxylase, an enzyme involved in the formation of aldehydes from amino acids by Lactococcus lactis.FEMS microbiology letters, 238 2
F. Kunst, N. Ogasawara, I. Moszer, A. Albertini, G. Alloni, V. Azevedo, M. Bertero, M. Bertero, P. Bessières, A. Bolotin, S. Borchert, R. Borriss, L. Boursier, A. Brans, M. Braun, S. Brignell, S. Bron, S. Brouillet, S. Brouillet, C. Bruschi, B. Caldwell, V. Capuano, N. Carter, S-K. Choi, Jean-Jacques Codani, I. Connerton, N. Cummings, R. Daniel, F. Denizot, K. Devine, A. Düsterhöft, S. Ehrlich, P. Emmerson, K. Entian, J. Errington, C. Fabret, E. Ferrari, D. Foulger, C. Fritz, M. Fujita, Y. Fujita, S. Fuma, A. Galizzi, N. Galleron, S. Ghim, P. Glaser, A. Goffeau, E. Golightly, G. Grandi, G. Guiseppi, B. Guy, K. Haga, J. Haiech, C. Harwood, A. Hénaut, H. Hilbert, S. Holsappel, S. Hosono, M. Hullo, M. Itaya, Louis Jones, B. Joris, D. Karamata, Y. Kasahara, M. Klaerr-Blanchard, C. Klein, Y. Kobayashi, P. Koetter, G. Koningstein, Susanne Krogh, M. Kumano, K. Kurita, A. Lapidus, S. Lardinois, J. Lauber, V. Lazarevic, S. Lee, A. Lévine, H. Liu, S. Masuda, C. Mauël, C. Médigue, C. Médigue, N. Medina, R. Mellado, M. Mizuno, D. Moestl, S. Nakai, M. Noback, D. Noone, M. O'Reilly, K. Ogawa, A. Ogiwara, B. Oudega, S. Park, V. Parro, T. Pohl, D. Portetelle, S. Porwollik, A. Prescott, E. Presecan, P. Pujić, B. Purnelle, G. Rapoport, M. Rey, Stacey Reynolds, M. Rieger, C. Rivolta, E. Rocha, E. Rocha, B. Roche, M. Rose, Y. Sadaie, Toshitada Sato, E. Scanlan, S. Schleich, R. Schroeter, F. Scoffone, J. Sekiguchi, A. Sękowska, S. Séror, P. Serror, B. Shin, B. Soldo, A. Sorokin, E. Tacconi, T. Takagi, H. Takahashi, K. Takemaru, M. Takeuchi, A. Tamakoshi, T. Tanaka, P. Terpstra, A. Tognoni, V. Tosato, S. Uchiyama, M. Vandenbol, F. Vannier, A. Vassarotti, A. Viari, R. Wambutt, E. Wedler, H. Wedler, T. Weitzenegger, P. Winters, A. Wipat, Hiroki Yamamoto, K. Yamane, K. Yasumoto, Katsunori Yata, K. Yoshida, H. Yoshikawa, E. Zumstein, H. Yoshikawa, Antoine Danchin (1997)
The complete genome sequence of the Gram-positive bacterium Bacillus subtilisNature, 390
A. Guérout-Fleury, N. Frandsen, P. Stragier (1996)
Plasmids for ectopic integration in Bacillus subtilis.Gene, 180 1-2
H. Ramos, T. Hoffmann, M. Marino, H. Nedjari, Elena Presecan-Siedel, O. Dreesen, P. Glaser, D. Jahn (2000)
Fermentative Metabolism of Bacillus subtilis: Physiology and Regulation of Gene ExpressionJournal of Bacteriology, 182
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
P. Wang, R. Doi (1984)
Overlapping promoters transcribed by bacillus subtilis sigma 55 and sigma 37 RNA polymerase holoenzymes during growth and stationary phases.The Journal of biological chemistry, 259 13
M Plaza de la (2004)
367FEMS Microbiol Lett, 238
N. Gollop, Batsheva Damri, D. Chipman, Z. Barak (1990)
Physiological implications of the substrate specificities of acetohydroxy acid synthases from varied organismsJournal of Bacteriology, 172
M. Nakano, Y. Dailly, P. Zuber, D. Clark (1997)
Characterization of anaerobic fermentative growth of Bacillus subtilis: identification of fermentation end products and genes required for growthJournal of Bacteriology, 179
C. Anagnostopoulos, J. Spizizen (1961)
REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILISJournal of Bacteriology, 81
Jia Jian, Shaowei Zhang, Zhen-Yu Shi, Wei Wang, Guo-Qiang Chen, Qiong Wu (2010)
Production of polyhydroxyalkanoates by Escherichia coli mutants with defected mixed acid fermentation pathwaysApplied Microbiology and Biotechnology, 87
S. Atsumi, Zhen Li, J. Liao (2009)
Acetolactate Synthase from Bacillus subtilis Serves as a 2-Ketoisovalerate Decarboxylase for Isobutanol Biosynthesis in Escherichia coliApplied and Environmental Microbiology, 75
X. Qin, H. Taber (1996)
Transcriptional regulation of the Bacillus subtilis menp1 promoterJournal of Bacteriology, 178
S. Mukherji, A. Oudenaarden (2009)
Synthetic biology: understanding biological design from synthetic circuitsNature Reviews Genetics, 10
Xinyao Liu, D. Brune, W. Vermaas, R. Curtiss (2010)
Production and secretion of fatty acids in genetically engineered cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America
W. Mu, Feng-bao Liu, Jianghua Jia, Chao-Peng Chen, Tao Zhang, B. Jiang (2009)
3-Phenyllactic acid production by substrate feeding and pH-control in fed-batch fermentation of Lactobacillus sp. SK007.Bioresource technology, 100 21
Curt Fischer, D. Klein‐Marcuschamer, G. Stephanopoulos (2008)
Selection and optimization of microbial hosts for biofuels production.Metabolic engineering, 10 6
In the present work, Bacillus subtilis was engineered as the cell factory for isobutanol production due to its high tolerance to isobutanol. Initially, an efficient heterologous Ehrlich pathway controlled by the promoter P43 was introduced into B. subtilis for the isobutanol biosynthesis. Further, investigation of acetolactate synthase of B. subtilis, ketol-acid reductoisomerase, and dihydroxy-acid dehydratase of Corynebacterium glutamicum responsible for 2-ketoisovalerate precursor biosynthesis showed that acetolactate synthase played an important role in isobutanol biosynthesis. The overexpression of acetolactate synthase led to a 2.8-fold isobutanol production compared with the control. Apart from isobutanol, alcoholic profile analysis also confirmed the existence of 1.21 g/L ethanol, 1.06 g/L 2-phenylethanol, as well as traces of 2-methyl-1-butanol and 3-methyl-1-butanol in the fermentation broth. Under microaerobic condition, the engineered B. subtilis produced up to 2.62 g/L isobutanol in shake-flask fed-batch fermentation, which was 21.3% higher than that in batch fermentation.
Applied Microbiology and Biotechnology – Springer Journals
Published: Apr 28, 2011
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.