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GATE‐16, a membrane transport modulator, interacts with NSF and the Golgi v‐SNARE GOS‐28

GATE‐16, a membrane transport modulator, interacts with NSF and the Golgi v‐SNARE GOS‐28 Membrane proteins located on vesicles (v‐SNAREs) and on the target membrane (t‐SNAREs) mediate specific recognition and, possibly, fusion between a transport vesicle and its target membrane. The activity of SNARE molecules is regulated by several soluble cytosolic proteins. We have cloned a bovine brain cDNA encoding a conserved 117 amino acid polypeptide, denoted Golgi‐associated ATPase Enhancer of 16 kDa (GATE‐16), that functions as a soluble transport factor. GATE‐16 interacts with N‐ethylmaleimidesensitive factor (NSF) and significantly stimulates its ATPase activity. It also interacts with the Golgi v‐SNARE GOS‐28 in an NSF‐dependent manner. We propose that GATE‐16 modulates intra‐Golgi transport through coupling between NSF activity and SNAREs activation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The EMBO Journal Wiley

GATE‐16, a membrane transport modulator, interacts with NSF and the Golgi v‐SNARE GOS‐28

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References (65)

Publisher
Wiley
Copyright
Copyright © 2013 Wiley Periodicals, Inc
ISSN
0261-4189
eISSN
1460-2075
DOI
10.1093/emboj/19.7.1494
pmid
10747018
Publisher site
See Article on Publisher Site

Abstract

Membrane proteins located on vesicles (v‐SNAREs) and on the target membrane (t‐SNAREs) mediate specific recognition and, possibly, fusion between a transport vesicle and its target membrane. The activity of SNARE molecules is regulated by several soluble cytosolic proteins. We have cloned a bovine brain cDNA encoding a conserved 117 amino acid polypeptide, denoted Golgi‐associated ATPase Enhancer of 16 kDa (GATE‐16), that functions as a soluble transport factor. GATE‐16 interacts with N‐ethylmaleimidesensitive factor (NSF) and significantly stimulates its ATPase activity. It also interacts with the Golgi v‐SNARE GOS‐28 in an NSF‐dependent manner. We propose that GATE‐16 modulates intra‐Golgi transport through coupling between NSF activity and SNAREs activation.

Journal

The EMBO JournalWiley

Published: Mar 3, 2000

Keywords: ; ; ;

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