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M. Köllner, J. Wolfrum (1992)
How many photons are necessary for fluorescence-lifetime measurements?Chemical Physics Letters, 200
K. Carlsson, A. Liljeborg, R. Andersson, H. Brismar (2000)
Confocal pH imaging of microscopic specimens using fluorescence lifetimes and phase fluorometry: influence of parameter choice on system performanceJournal of Microscopy, 199
K. Carlsson, N. Aslund, K. Mossberg, J. Philip (1994)
Simultaneous confocal recording of multiple fluorescent labels with improved channel separationJournal of Microscopy, 176
H. Brismar, Brun UIfhake (1997)
Fluorescence lifetime measurements in confocal microscopy of neurons labeled with multiple fluorophoresNature Biotechnology, 15
K. König, Peter So, W. Mantulin, B. Tromberg, Enrico Gratton (1996)
Two‐photon excited lifetime imaging of autofluorescence in cells during UV A and NIR photostressJournal of Microscopy, 183
G. Krishnamoorthy, A. Srivastava (1997)
INTRACELLULAR DYNAMICS SEEN THROUGH TIME-RESOLVED FLUORESCENCE MICROSCOPYCurrent Science, 72
T. Gadella, T. Jovin, R. Clegg (1993)
Fluorescence lifetime imaging microscopy (FLIM): Spatial resolution of microstructures on the nanosecond time scaleBiophysical Chemistry, 48
A. Draaijer, R. Sanders, H. Gerritsen (1995)
Fluorescence Lifetime Imaging, a New Tool in Confocal Microscopy
R. Ballew, J. Demas (1989)
An error analysis of the rapid lifetime determination method for the evaluation of single exponential decaysAnalytical Chemistry, 61
E. Buurman, R. Sanders, A. Draaijer, H. Gerritsen, J. Veen, P. Houpt, Y. Levine (1992)
Fluorescence lifetime imaging using a confocal laser scanning microscopeScanning, 14
J. Lakowicz, H. Szmacinski (1993)
Fluorescence lifetime-based sensing of pH, Ca2+, K+ and glucoseSensors and Actuators B-chemical, 11
A. Squire, P. Bastiaens (1999)
Three dimensional image restoration in fluorescence lifetime imaging microscopyJournal of Microscopy, 193
R. Müller, C. Zander, M. Sauer, M. Deimel, D. Ko, S. Siebert, J. Arden-Jacob, G. Deltau, N. Marx, K. Drexhage, J. Wolfrum (1996)
Time-resolved identification of single molecules in solution with a pulsed semiconductor diode laserChemical Physics Letters, 262
M. Sauer, J. Arden-Jacob, K. Drexhage, Florian G�bel, U. Lieberwirth, Klaus M�hlegger, Ralph M�ller, J�rgen Wolfrum, C. Zander (1998)
Time‐resolved identification of individual mononucleotide molecules in aqueous solution with pulsed semiconductor lasersBioimaging, 6
C. Oord, H. Gerritsen, F. Rommerts, D. Shaw, I. Munro, Y. Levine (1995)
Micro-Volume Time-Resolved Fluorescence Spectroscopy Using a Confocal Synchrotron Radiation MicroscopeApplied Spectroscopy, 49
J. Lakowicz, K. Berndt (1991)
Lifetime‐selective fluorescence imaging using an rf phase‐sensitive cameraReview of Scientific Instruments, 62
D. Piston, D. Sandison, W. Webb (1992)
Time-resolved fluorescence imaging and background rejection by two-photon excitation in laser-scanning microscopy, 1640
J. Sytsma, J. Vroom, C. Grauw, H. Gerritsen (1998)
Time‐gated fluorescence lifetime imaging and microvolume spectroscopy using two‐photon excitationJournal of Microscopy, 191
P. So, T. French, Wm Yu, K. Berland, C. Dong, E. Gratton (1995)
Time-resolved fluorescence microscopy using two-photon excitationBioimaging, 3
T. French, P. So, Donald Weaver, T. Coelho-Sampaio, E. Gratton, E. Voss, J. Carrero (1997)
Two‐photon fluorescence lifetime imaging microscopy of macrophage‐mediated antigen processingJournal of Microscopy, 185
(1993)
Act. B
C. Grauw, H. Gerritsen (2001)
Multiple Time-Gate Module for Fluorescence Lifetime ImagingApplied Spectroscopy, 55
Carlsson, Liljeborg (1998)
Simultaneous confocal lifetime imaging of multiple fluorophores using the intensity‐modulated multiple‐wavelength scanning (IMS) techniqueJournal of Microscopy, 191
A. Buist, M. Müller, E. Gijsbers, G. Brakenhoff, T. Sosnowski, T. Norris, J. Squier (1997)
Double‐pulse fluorescence lifetime measurementsJournal of Microscopy, 186
Using Monte-Carlo methods, we have investigated the signal- to-noise ratio obtainable for different fluorescence lifetime imaging methods. Quantum noise limited performance and mono-exponential decays were assumed. We have also investigated the importance of parameter choice and implementation for the different methods. In addition, our simulations were in many cases compared with analytical theoretical investigations. The results from the simulations proved to be in good agreement with the theoretical results. It was found that all the investigated lifetime imaging methods have the potential to produce a high signal-to-noise ratio, but careful attention must be paid to implementation method and parameter choice in order to get optimal results.
Proceedings of SPIE – SPIE
Published: May 28, 2002
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