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B. Pan, R. Johnstone (1983)
Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: Selective externalization of the receptorCell, 33
Michel Vidal, Paul Mangeat, Dick Hoekstra (1997)
Aggregation reroutes molecules from a recycling to a vesicle-mediated secretion pathway during reticulocyte maturation.Journal of cell science, 110 ( Pt 16)
I. Mills, S. Urbé, M. Clague (2001)
Relationships between EEA1 binding partners and their role in endosome fusion.Journal of cell science, 114 Pt 10
B. Pan, K. Teng, WU Choan, M. Adam, R. Johnstone (1985)
Electron microscopic evidence for externalization of the transferrin receptor in vesicular form in sheep reticulocytesThe Journal of Cell Biology, 101
C. Futter, Adele Pearse, L. Hewlett, C. Hopkins (1996)
Multivesicular endosomes containing internalized EGF-EGF receptor complexes mature and then fuse directly with lysosomesThe Journal of Cell Biology, 132
A. Savina, M. Furlán, M. Vidal, M. Colombo (2003)
Exosome Release Is Regulated by a Calcium-dependent Mechanism in K562 Cells*Journal of Biological Chemistry, 278
L. Zitvogel, A. Régnault, A. Lozier, J. Wolfers, C. Flament, D. Tenza, P. Ricciardi-Castagnoli, G. Raposo, S. Amigorena (1998)
Eradication of established murine tumors using a novel cell-free vaccine: dendritic cell derived exosomesNature Medicine, 4
Wei Chen, Yan Feng, Dayue Chen, A. Wandinger-Ness (1998)
Rab11 is required for trans-golgi network-to-plasma membrane transport and a preferential target for GDP dissociation inhibitor.Molecular biology of the cell, 9 11
A. Gassart, C. Géminard, Benoît Février, G. Raposo, M. Vidal (2003)
Lipid raft-associated protein sorting in exosomes.Blood, 102 13
N. Bishop (2003)
Dynamics of endosomal sorting.International review of cytology, 232
Mindong Ren, Gen-Xin Xu, J. Zeng, C. Lemos-Chiarandini, M. Adesnik, D. Sabatini (1998)
Hydrolysis of GTP on rab11 is required for the direct delivery of transferrin from the pericentriolar recycling compartment to the cell surface but not from sorting endosomes.Proceedings of the National Academy of Sciences of the United States of America, 95 11
R. Jahn, Helmut Grubmüller (2002)
Membrane fusion.Current opinion in cell biology, 14 4
C. Théry, Livine Duban, É. Segura, P. Véron, O. Lantz, S. Amigorena (2002)
Indirect activation of naïve CD4+ T cells by dendritic cell–derived exosomesNature Immunology, 3
M. Vidal, P. Stahl (1993)
The small GTP-binding proteins Rab4 and ARF are associated with released exosomes during reticulocyte maturation.European journal of cell biology, 60 2
S. Gerber, T. Südhof (2002)
Molecular determinants of regulated exocytosis.Diabetes, 51 Suppl 1
C. Holroyd, U. Kistner, W. Annaert, R. Jahn (1999)
Fusion of endosomes involved in synaptic vesicle recycling.Molecular biology of the cell, 10 9
H. Heijnen, Anja Schiel, R. Fijnheer, H. Geuze, J. Sixma (1999)
Activated platelets release two types of membrane vesicles: microvesicles by surface shedding and exosomes derived from exocytosis of multivesicular bodies and alpha-granules.Blood, 94 11
C. Géminard, A. Gassart, L. Blanc, M. Vidal (2004)
Degradation of AP2 During Reticulocyte Maturation Enhances Binding of Hsc70 and Alix to a Common Site on TfR for Sorting into ExosomesTraffic, 5
B. Stein, K. Bensch, H. Sussman (1984)
Complete inhibition of transferrin recycling by monensin in K562 cells.The Journal of biological chemistry, 259 23
R. Roberts, M. Barbieri, K. Pryse, M. Chua, J. Morisaki, P. Stahl (1999)
Endosome fusion in living cells overexpressing GFP-rab5.Journal of cell science, 112 ( Pt 21)
P. Brennwald (2000)
Reversal of fortune. Do Rab GTPases act on the target membrane?The Journal of Cell Biology, 149
M. Babst, D. Katzmann, W. Snyder, B. Wendland, S. Emr (2002)
Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body.Developmental cell, 3 2
A. Savina, M. Vidal, M. Colombo (2002)
The exosome pathway in K562 cells is regulated by Rab11.Journal of cell science, 115 Pt 12
E. Green, E. Ramm, N. Riley, D. Spiro, J. Goldenring, M. Wessling-Resnick (1997)
Rab11 is associated with transferrin-containing recycling compartments in K562 cells.Biochemical and biophysical research communications, 239 2
C. Raiborg, Kristi Bache, A. Mehlum, E. Stang, H. Stenmark (2001)
Hrs recruits clathrin to early endosomesThe EMBO Journal, 20
C. Raiborg, H. Stenmark (2002)
Hrs and endocytic sorting of ubiquitinated membrane proteins.Cell structure and function, 27 6
J. Willem, M. Beest, G. Scherphof, D. Hoekstra (1990)
A non-exchangeable fluorescent phospholipid analog as a membrane traffic marker of the endocytic pathway.European journal of cell biology, 53 1
R. Diaz, L. Mayorga, L. Mayorga, P. Stahl (1989)
In vitro clustering and multiple fusion among macrophage endosomes.The Journal of biological chemistry, 264 22
Toshihide Kobayashi, E. Stang, K. Fang, P. Moerloose, R. Parton, J. Gruenberg (1998)
A lipid associated with the antiphospholipid syndrome regulates endosome structure and functionNature, 392
S. Urbé, L. Huber, M. Zerial, S. Tooze, R. Parton (1993)
Rab11, a small GTPase associated with both constitutive and regulated secretory pathways in PC12 cellsFEBS Letters, 334
R. Piper, J. Luzio (2001)
Late Endosomes: Sorting and Partitioning in Multivesicular BodiesTraffic, 2
R. Wubbolts, Rachel Leckie, P. Veenhuizen, G. Schwarzmann, W. Möbius, Joerg Hoernschemeyer, J. Slot, H. Geuze, W. Stoorvogel (2003)
Proteomic and Biochemical Analyses of Human B Cell-derived ExosomesThe Journal of Biological Chemistry, 278
M. Zerial, H. McBride (2001)
Rab proteins as membrane organizersNature Reviews Molecular Cell Biology, 2
T. Voets (2000)
Dissection of Three Ca2+-Dependent Steps Leading to Secretion in Chromaffin Cells from Mouse Adrenal SlicesNeuron, 28
Brennwald (2000)
Reversal of fortune. Do Rab GTPases act on the target membrane?J Cell Biol, 149
O. Ullrich, S. Reinsch, Sylvie Urb, M. Zerial, R. ton (1996)
Rab11 regulates recycling through the pericentriolar recycling endosomeThe Journal of Cell Biology, 135
Jahn (2003)
Membrane fusionCell, 112
(2000)
Follicular dendritic cells carry MHC class II-expressing microvesicles at their surface
Kristi Bache, C. Raiborg, A. Mehlum, H. Stenmark (2003)
STAM and Hrs Are Subunits of a Multivalent Ubiquitin-binding Complex on Early Endosomes*The Journal of Biological Chemistry, 278
W. Möbius, E. Donselaar, Y. Ohno‐Iwashita, Y. Shimada, H. Heijnen, J. Slot, H. Geuze (2003)
Recycling Compartments and the Internal Vesicles of Multivesicular Bodies Harbor Most of the Cholesterol Found in the Endocytic PathwayTraffic, 4
G. Raposo, H. Nijman, W. Stoorvogel, R. Liejendekker, C. Harding, C. Melief, H. Geuze (1996)
B lymphocytes secrete antigen-presenting vesiclesThe Journal of Experimental Medicine, 183
R. Johnstone, A. Bianchini, Kathy Teng (1989)
Reticulocyte maturation and exosome release: transferrin receptor containing exosomes shows multiple plasma membrane functions.Blood, 74 5
R. Johnstone (1996)
Cleavage of the transferrin receptor by human granulocytes: Preferential proteolysis of the exosome‐bound TFRJournal of Cellular Physiology, 168
D. Katzmann, M. Babst, S. Emr (2001)
Ubiquitin-Dependent Sorting into the Multivesicular Body Pathway Requires the Function of a Conserved Endosomal Protein Sorting Complex, ESCRT-ICell, 106
E. Dömötör, N. Abbott, V. Ádám‐Vizi (1999)
Na+–Ca2+ exchange and its implications for calcium homeostasis in primary cultured rat brain microvascular endothelial cellsThe Journal of Physiology, 515
Stephen Felder, Karen Miller, G. Moehren, A. Ullrich, J. Schlessinger, Colin Hopkins (1990)
Kinase activity controls the sorting of the epidermal growth factor receptor within the multivesicular bodyCell, 61
C. Peters, A. Mayer (1998)
Ca2+/calmodulin signals the completion of docking and triggers a late step of vacuole fusionNature, 396
S. Rieu, C. Géminard, H. Rabesandratana, J. Sainte‐Marie, M. Vidal (2000)
Exosomes released during reticulocyte maturation bind to fibronectin via integrin α4β1FEBS Journal, 267
M. Kleijmeer, S. Morkowski, Janice Griffith, A. Rudensky, H. Geuze (1997)
Major Histocompatibility Complex Class II Compartments in Human and Mouse B Lymphoblasts Represent Conventional Endocytic CompartmentsThe Journal of Cell Biology, 139
Hirotami Matsuo, J. Chevallier, N. Mayran, I. Blanc, C. Ferguson, J. Fauré, N. Blanc, S. Matile, J. Dubochet, R. Sadoul, R. Parton, F. Vilbois, J. Gruenberg (2004)
Role of LBPA and Alix in Multivesicular Liposome Formation and Endosome OrganizationScience, 303
B. Mullock, N. Bright, C. Fearon, S. Gray, J. Luzio (1998)
Fusion of Lysosomes with Late Endosomes Produces a Hybrid Organelle of Intermediate Density and Is NSF DependentThe Journal of Cell Biology, 140
R. Johnstone, M. Adam, B. Pan (1984)
The fate of the transferrin receptor during maturation of sheep reticulocytes in vitro.Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire, 62 11
C. Harding, J. Heuser, P. Stahl (1983)
Receptor-mediated endocytosis of transferrin and recycling of the transferrin receptor in rat reticulocytesThe Journal of Cell Biology, 97
R. Johnstone, M. Adam, J. Hammond, L. Orr, C. Turbide (1987)
Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles (exosomes).The Journal of biological chemistry, 262 19
M. Babst, D. Katzmann, Eden Estepa-Sabal, T. Meerloo, S. Emr (2002)
Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting.Developmental cell, 3 2
J. Gruenberg, K. Howell (1989)
Membrane traffic in endocytosis: insights from cell-free assays.Annual review of cell biology, 5
C. Géminard, Francine Nault, R. Johnstone, M. Vidal (2001)
Characteristics of the Interaction between Hsc70 and the Transferrin Receptor in Exosomes Released during Reticulocyte Maturation*The Journal of Biological Chemistry, 276
M. Khvotchev, Mindong Ren, S. Takamori, R. Jahn, T. Südhof (2003)
Divergent Functions of Neuronal Rab11b in Ca2+-Regulated versus Constitutive ExocytosisThe Journal of Neuroscience, 23
I. Ho, G. Ellman (1969)
TRITON SOLUBILIZED ACETYLCHOLINESTERASE OF BRAINJournal of Neurochemistry, 16
S. Pfeffer (1994)
Rab GTPases: master regulators of membrane trafficking.Current opinion in cell biology, 6 4
J. Gruenberg, H. Stenmark (2004)
The biogenesis of multivesicular endosomesNature Reviews Molecular Cell Biology, 5
C. Harding, M. Levy, P. Stahl (1985)
Morphological analysis of ligand uptake and processing: the role of multivesicular endosomes and CURL in receptor-ligand processing.European journal of cell biology, 36 2
Multivesicular bodies (MVBs) are membranous structures within 60–100 nm diameter vesicles accumulate. MVBs are generated after invagination and pinching off of the endosomal membrane in the lumen of the vacuole. In certain cell types, fusion of MVBs with the plasma membrane results in the release of the internal vesicles called exosomes. In this report we have examined how an increase in cytosolic calcium affects the development of MVBs and exosome release in K562 cells overexpressing GFP‐Rab11 wt or its mutants. In cells overexpressing the Rab11Q70 L mutant or Rab11 wt, an increase in the cytosolic calcium concentration induced by monensin caused a marked enlargement of the MVBs. This effect was abrogated by the membrane permeant calcium chelator BAPTA‐AM. We also examined the behavior of MVBs in living cells by time lapse confocal microscopy. Many MVBs, decorated by wt or Q70L mutant GFP‐Rab11, were docked and ready to fuse in the presence of a calcium chelator. This observation suggests that Rab11 is acting in the tethering/docking of MVBs to promote homotypic fusion, but that the final fusion reaction requires the presence of calcium. Additionally, a rise in intracellular calcium concentration enhanced exosome secretion in Rab11 wt overexpressing cells and reversed the inhibition of the mutants. The results suggest that both Rab11 and calcium are involved in the homotypic fusion of MVBs.
Traffic – Wiley
Published: Feb 1, 2005
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