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Identification of a novel cis‐regulatory element for UV‐B‐induced transcription in Arabidopsis

Identification of a novel cis‐regulatory element for UV‐B‐induced transcription in Arabidopsis Ultraviolet‐B light (UV‐B) regulates the expression of genes in a wavelength‐ and fluence rate‐dependent fashion. A signaling pathway consisting of CONSTITUTIVE PHOTOMORPHOGENESIS 1 (COP1) and UV RESISTANCE LOCUS 8 (UVR 8) mediates responsiveness to longer wavelength, low intensity UV‐B light‐activating, for example, HY5 gene expression. By contrast, transcription of another group of genes, including ANAC13, modulated by shorter wavelength, higher intensity UV‐B is controlled by a yet unknown and largely COP1‐independent signaling cascade. Here we provide evidence by promoter deletion analysis, and characterization of genetic mutants displaying aberrant expression patterns, that two cis‐regulatory elements, designated MREANAC13 and UVBoxANAC13, are required for maximal UV‐B induction of the ANAC13 gene in transgenic plants. These elements are located in the proximal 150‐bp region of the ANAC13 promoter. They show no significant similarity to each other; the putative MREANAC13 (‐AACCTT‐) is closely related to MRECHS (‐AACCTA‐) found in the CHALCONE SYNTHASE (CHS) gene, whereas UVBoxANAC13 (with core sequence CAAG) represents a novel cis‐regulatory element. The novel UVBoxANAC13 sequence is significantly enriched in the promoter region of a subset of UV‐B‐induced genes with similar activation properties as ANAC13. In addition, we demonstrate that expression of a chimeric gene containing only the dimerized 12‐mer containing UVBoxANAC13 fused to a minimal CaMV35S promoter/luciferase reporter is (i) efficiently induced by shorter wavelength, higher intensity UV‐B, but (ii) does not respond either to longer wavelength UV‐B and red light or (iii) to abscisic acid treatment and osmotic, salt, heat and cold stresses. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Plant Journal Wiley

Identification of a novel cis‐regulatory element for UV‐B‐induced transcription in Arabidopsis

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References (40)

Publisher
Wiley
Copyright
Copyright © 2008 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0960-7412
eISSN
1365-313X
DOI
10.1111/j.1365-313X.2008.03435.x
pmid
18266923
Publisher site
See Article on Publisher Site

Abstract

Ultraviolet‐B light (UV‐B) regulates the expression of genes in a wavelength‐ and fluence rate‐dependent fashion. A signaling pathway consisting of CONSTITUTIVE PHOTOMORPHOGENESIS 1 (COP1) and UV RESISTANCE LOCUS 8 (UVR 8) mediates responsiveness to longer wavelength, low intensity UV‐B light‐activating, for example, HY5 gene expression. By contrast, transcription of another group of genes, including ANAC13, modulated by shorter wavelength, higher intensity UV‐B is controlled by a yet unknown and largely COP1‐independent signaling cascade. Here we provide evidence by promoter deletion analysis, and characterization of genetic mutants displaying aberrant expression patterns, that two cis‐regulatory elements, designated MREANAC13 and UVBoxANAC13, are required for maximal UV‐B induction of the ANAC13 gene in transgenic plants. These elements are located in the proximal 150‐bp region of the ANAC13 promoter. They show no significant similarity to each other; the putative MREANAC13 (‐AACCTT‐) is closely related to MRECHS (‐AACCTA‐) found in the CHALCONE SYNTHASE (CHS) gene, whereas UVBoxANAC13 (with core sequence CAAG) represents a novel cis‐regulatory element. The novel UVBoxANAC13 sequence is significantly enriched in the promoter region of a subset of UV‐B‐induced genes with similar activation properties as ANAC13. In addition, we demonstrate that expression of a chimeric gene containing only the dimerized 12‐mer containing UVBoxANAC13 fused to a minimal CaMV35S promoter/luciferase reporter is (i) efficiently induced by shorter wavelength, higher intensity UV‐B, but (ii) does not respond either to longer wavelength UV‐B and red light or (iii) to abscisic acid treatment and osmotic, salt, heat and cold stresses.

Journal

The Plant Journal – Wiley

Published: May 1, 2008

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