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Mouse deoxyribonuclease I (DNase I): Biochemical and immunological characterization, cDNA structure and tissue distribution

Mouse deoxyribonuclease I (DNase I): Biochemical and immunological characterization, cDNA... Mouse urinary deoxyribonuclease I (DNase I) resembles rat and human DNase Is in terms of its proteochemical and enzymological properties. Furthermore, mouse DNase I was demonstrated to be immunologically closer to the rat than to the human enzyme. A 1176 bp full length cDNA encoding mouse DNase I was constructed from RNA obtained from the kidney and parotid glands. The amino acid sequence up to the 45th residue from the N‐terminal of the mature enzyme was identical to that deduced from the cDNA sequence. This DNase I was distributed most densely in the parotid glands from the standpoint of both enzyme activity and gene transcript levels. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png IUBMB Life Wiley

Mouse deoxyribonuclease I (DNase I): Biochemical and immunological characterization, cDNA structure and tissue distribution

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 1997 International Union of Biochemistry and Molecular Biology
ISSN
1521-6543
eISSN
1521-6551
DOI
10.1080/15216549700202441
Publisher site
See Article on Publisher Site

Abstract

Mouse urinary deoxyribonuclease I (DNase I) resembles rat and human DNase Is in terms of its proteochemical and enzymological properties. Furthermore, mouse DNase I was demonstrated to be immunologically closer to the rat than to the human enzyme. A 1176 bp full length cDNA encoding mouse DNase I was constructed from RNA obtained from the kidney and parotid glands. The amino acid sequence up to the 45th residue from the N‐terminal of the mature enzyme was identical to that deduced from the cDNA sequence. This DNase I was distributed most densely in the parotid glands from the standpoint of both enzyme activity and gene transcript levels.

Journal

IUBMB Life – Wiley

Published: Jun 1, 1997

Keywords: Deoxyribonuclease I (DNase I); Mouse; cDNA; Properties; Distribution; PCR

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