Access the full text.
Sign up today, get DeepDyve free for 14 days.
D. Parkin, P. Pisani, J. Ferlay (1999)
Estimates of the worldwide incidence of 25 major cancers in 1990International Journal of Cancer, 80
P. Pisani, D. Parkin, F. Bray, J. Ferlay (1999)
Erratum: Estimates of the worldwide mortality from 25 cancers in 1990. Int. J. Cancer, 83, 18–29 (1999).International Journal of Cancer, 83
J. Joyce, J. Tung, C. Przysiecki, J. Cook, E. Lehman, J. Sands, K. Jansen, P. Keller (1999)
The L1 Major Capsid Protein of Human Papillomavirus Type 11 Recombinant Virus-like Particles Interacts with Heparin and Cell-surface Glycosaminoglycans on Human Keratinocytes*The Journal of Biological Chemistry, 274
N. Muñoz, F. Bosch, S. Sanjosé, R. Herrero, X. Castellsagué, K. Shah, P. Snijders, C. Meijer (2003)
Epidemiologic classification of human papillomavirus types associated with cervical cancer.The New England journal of medicine, 348 6
F. Sherman (1998)
An Introduction to the Genetics and Molecular Biology of the Yeast Saccharomyces cerevisiae
T. Sasagawa, P. Pushko, G. Steers, Steve Gschmeissner, M. Hajibagheri, J. Finch, L. Crawford, M. Tommasino (1995)
Synthesis and assembly of virus-like particles of human papillomaviruses type 6and Type 16 in fission yeast Schizosaccharomyces pombeVirology, 206
Se Kim, H. Jeong, Sue-N. Park, Hong-Jin Kim (2007)
Purification and immunogenicity study of human papillomavirus type 16 L1 protein in Saccharomyces cerevisiae.Journal of virological methods, 139 1
J. Cook, J. Joyce, H. George, L. Schultz, W. Hurni, K. Jansen, Robert Hepler, C. Ip, R. Lowe, P. Keller, E. Lehman (1999)
Purification of virus-like particles of recombinant human papillomavirus type 11 major capsid protein L1 from Saccharomyces cerevisiae.Protein expression and purification, 17 3
J. Walboomers, M. Jacobs, M. Manos, F. Bosch, J. Kummer, K. Shah, P. Snijders, J. Peto, C. Meijer, N. Muñoz (1999)
Human papillomavirus is a necessary cause of invasive cervical cancer worldwideThe Journal of Pathology, 189
C. Volpers, P. Schirmacher, R. Streeck, M. Sapp (1994)
Assembly of the major and the minor capsid protein of human papillomavirus type 33 into virus-like particles and tubular structures in insect cells.Virology, 200 2
P. Pisani, D. Parkin, F. Bray, J. Ferlay (1999)
Estimates of the worldwide mortality from 25 cancers in 1990International Journal of Cancer, 83
F. Bosch, M. Manos, N. Muñoz, M. Sherman, A. Jansen, J. Peto, M. Schiffman, V. Moreno, R. Kurman, K. Shah (1995)
Prevalence of Human Papillomavirus in Cervical Cancer: a Worldwide PerspectiveJournal of the National Cancer Institute, 87
Copyright © 1998, American Society for Microbiology Yeast Carbon Catabolite Repression†
T. Wright, F. Bosch, E. Franco, J. Cuzick, J. Schiller, G. Garnett, A. Meheus (2006)
Chapter 30: HPV vaccines and screening in the prevention of cervical cancer; conclusions from a 2006 workshop of international experts.Vaccine, 24 Suppl 3
J. Suzich, Shin-je Ghim, F. Palmer-Hill, W. White, J. Tamura, J. Bell, J. Newsome, A. Jenson, R. Schlegel (1995)
Systemic immunization with papillomavirus L1 protein completely prevents the development of viral mucosal papillomas.Proceedings of the National Academy of Sciences of the United States of America, 92 25
Yevgeniy Studentsov, M. Schiffman, H. Strickler, G. Ho, Y. Pang, J. Schiller, R. Herrero, R. Burk (2002)
Enhanced Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Virus-Like Particles of Human PapillomavirusJournal of Clinical Microbiology, 40
R. Kirnbauer, L. Chandrachud, B. O'Neil, E. Wagner, G. Grindlay, A. Armstrong, G. McGarvie, J. Schiller, D. Lowy, M. Campo (1996)
Virus-like particles of bovine papillomavirus type 4 in prophylactic and therapeutic immunization.Virology, 219 1
R. Kirnbauer, F. Booy, N. Cheng, D. Lowy, J. Schiller (1992)
Papillomavirus L1 major capsid protein self-assembles into virus-like particles that are highly immunogenic.Proceedings of the National Academy of Sciences of the United States of America, 89 24
S. Tabrizi, Ian Frazer, Suzanne Garland (2005)
Serologic response to human papillomavirus 16 among Australian women with high-grade cervical intraepithelial neoplasiaInternational Journal of Gynecologic Cancer, 16
F. Breitburd, Pierre Coursaget (1999)
Human papillomavirus vaccines.Seminars in cancer biology, 9 6
M. Neeper, K. Hofmann, K. Jansen (1996)
Expression of the major capsid protein of human papillomavirus type 11 in Saccharomyces cerevisae.Gene, 180 1-2
H. Mach, D. Volkin, R. Troutman, Bei Wang, Zheng Luo, K. Jansen, Li Shi (2006)
Disassembly and reassembly of yeast-derived recombinant human papillomavirus virus-like particles (HPV VLPs).Journal of pharmaceutical sciences, 95 10
M. Schiffman, H. Bauer, R. Hoover, A. Glass, D. Cadell, B. Rush, David Scott, M. Sherman, R. Kurman, S. Wacholder, Cynthia Stanton, M. Manos (1993)
Epidemiologic evidence showing that human papillomavirus infection causes most cervical intraepithelial neoplasia.Journal of the National Cancer Institute, 85 12
J. Park, Hyun-Mi Pyo, Sun-Woo Yoon, S. Baek, Sue-N. Park, Chul-Joong Kim, H. Poo (2002)
Production and Prophylactic Efficacy Study of Human Papillomavirus-like Particle Expressing HPV16 L1 Capsid ProteinJournal of Microbiology, 40
R. Rose, W. Bonnez, R. Reichman, R. Garcea (1993)
Expression of human papillomavirus type 11 L1 protein in insect cells: in vivo and in vitro assembly of viruslike particlesJournal of Virology, 67
M. Hagensee, N. Yaegashi, D. Galloway (1993)
Self-assembly of human papillomavirus type 1 capsids by expression of the L1 protein alone or by coexpression of the L1 and L2 capsid proteinsJournal of Virology, 67
Cervical cancer caused by human papillomavirus (HPV) might be successfully prevented by HPV vaccination and screening. HPV vaccination and HPV serology assays have been investigated using HPV virus-like particles (VLPs). In this study we produced HPV18 L1 VLPs in Saccharomyces cerevisiae and purified them. The HPV18 L1 gene was cloned into the yeast expression vector YEGα-HIR525, and transformed into Saccharomyces cerevisiae . Expression of HPV18 L1 protein was demonstrated by Western blotting. The HPV18 L1 protein was purified by ultracentrifugation, size-exclusion chromatography and cation-exchange chromatography, and was up to 95% pure. We showed by transmission electron microscopy that the purified protein self-assembled into VLPs. These findings should be useful for establishing vaccine efficacy as well as characterizing vaccine candidates, and may provide an international reference standard for HPV serology assays.
Archives of Pharmacal Research – Springer Journals
Published: Feb 1, 2008
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.