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Physiological regulation of the expression of a GLUT4 homolog in fish skeletal muscle

Physiological regulation of the expression of a GLUT4 homolog in fish skeletal muscle Abstract We have recently cloned a glucose transporter from brown trout muscle (btGLUT) with high sequence homology to mammalian GLUT4 that is predominantly expressed in red and white skeletal muscle, the two major sites of glucose uptake in trout. To study the physiological regulation of this putative fish GLUT4, we have investigated the expression of btGLUT in red and white skeletal muscle of trout in which blood insulin levels have been altered experimentally. The expression of btGLUT in red muscle increased significantly when insulin plasma levels were elevated by either insulin or arginine treatment and decreased significantly when insulin plasma levels were reduced either by fasting or by feeding a low-protein, high-carbohydrate diet. In contrast, the expression of btGLUT in white muscle was not affected by changes in the plasma levels of insulin. These results strongly suggest that insulin could be regulating the expression of btGLUT in trout red muscle in vivo and set the ground to test the hypothesis that btGLUT may be considered a GLUT4 homolog in fish. insulin glucose messenger ribonucleic acid trout Footnotes E.Capilla was supported by a predoctoral fellowship from the University of Barcelona. This study was supported by grants AGF98–0325, Q5RS-2000–30068, and 2001SGR-00122 to J. Gutiérrez and PB98–1249 to J. V. Planas. Address for reprint requests and other correspondence: J. V. Planas, Departament de Fisiologia, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain (E-mail: planas@porthos.bio.ub.es ). The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “ advertisement ” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. First published March 12, 2002;10.1152/ajpendo.00065.2002 Copyright © 2002 the American Physiological Society http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png AJP - Endocrinology and Metabolism The American Physiological Society

Physiological regulation of the expression of a GLUT4 homolog in fish skeletal muscle

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Publisher
The American Physiological Society
Copyright
Copyright © 2011 the American Physiological Society
ISSN
0193-1849
eISSN
1522-1555
DOI
10.1152/ajpendo.00065.2002
pmid
12067841
Publisher site
See Article on Publisher Site

Abstract

Abstract We have recently cloned a glucose transporter from brown trout muscle (btGLUT) with high sequence homology to mammalian GLUT4 that is predominantly expressed in red and white skeletal muscle, the two major sites of glucose uptake in trout. To study the physiological regulation of this putative fish GLUT4, we have investigated the expression of btGLUT in red and white skeletal muscle of trout in which blood insulin levels have been altered experimentally. The expression of btGLUT in red muscle increased significantly when insulin plasma levels were elevated by either insulin or arginine treatment and decreased significantly when insulin plasma levels were reduced either by fasting or by feeding a low-protein, high-carbohydrate diet. In contrast, the expression of btGLUT in white muscle was not affected by changes in the plasma levels of insulin. These results strongly suggest that insulin could be regulating the expression of btGLUT in trout red muscle in vivo and set the ground to test the hypothesis that btGLUT may be considered a GLUT4 homolog in fish. insulin glucose messenger ribonucleic acid trout Footnotes E.Capilla was supported by a predoctoral fellowship from the University of Barcelona. This study was supported by grants AGF98–0325, Q5RS-2000–30068, and 2001SGR-00122 to J. Gutiérrez and PB98–1249 to J. V. Planas. Address for reprint requests and other correspondence: J. V. Planas, Departament de Fisiologia, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain (E-mail: planas@porthos.bio.ub.es ). The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “ advertisement ” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. First published March 12, 2002;10.1152/ajpendo.00065.2002 Copyright © 2002 the American Physiological Society

Journal

AJP - Endocrinology and MetabolismThe American Physiological Society

Published: Jul 1, 2002

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