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I. Herskowitz (1987)
Functional inactivation of genes by dominant negative mutationsNature, 329
Dave Thompson, L. Melchers, K. Idler, R. Schilperoort, P. Hooykaas (1988)
Analysis of the complete nucleotide sequence of the Agrobacterium tumefaciens virB operon.Nucleic acids research, 16 10
(1987)
Agrobacterium-mediated transformation of germinating seeds of Arabidopsis thaliana: a nontissue culture approach
G. Gheysen, Raimundo Villarroel, M. Montagu (1991)
Illegitimate recombination in plants: a model for T-DNA integration.Genes & development, 5 2
J. Ward, D. Akiyoshi, D. Regier, A. Datta, M. Gordon, E. Nester (1988)
Characterization of the virB operon from an Agrobacterium tumefaciens Ti plasmid.The Journal of biological chemistry, 263 12
Reinhold Mayerhofer, Z. Koncz-Kálmán, Christiane, '. Nawrath, G. Bakkeren, Andreas Crameri, Karel Angelis, G. Redei, Jeff Schell, Barbara Hohn, C. Koncz (1991)
T‐DNA integration: a mode of illegitimate recombination in plants.The EMBO Journal, 10
T. Kosuge, C. Meredith, A. Hollaender, Claire Wilson (1983)
Genetic Engineering of Plants
J. Sambrook, E. Fritsch, T. Maniatis (2001)
Molecular Cloning: A Laboratory Manual
R. Morris (1986)
Genes Specifying Auxin and Cytokinin Biosynthesis in PhytopathogensAnnual Review of Plant Biology, 37
J. Sheng, V. Citovsky (1996)
Agrobacterium-plant cell DNA transport: have virulence proteins, will travel.The Plant cell, 8
R. Jefferson, T. Kavanagh, M. Bevan (1987)
GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.The EMBO Journal, 6
M. Bennett, A. Marchant, Haydn Green, S. May, Sally Ward, P. Millner, A. Walker, B. Schulz, K. Feldmann (1996)
Arabidopsis AUX1 Gene: A Permease-Like Regulator of Root GravitropismScience, 273
S. Filichkin, S. Gelvin (1993)
Formation of a putative relaxation intermediate during T‐DNA processing directed by the Agrobacterium tumefaciens VirD1, D2 endonucleaseMolecular Microbiology, 8
P. Krysan, Jeff Young, F. Tax, M. Sussman (1996)
Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport.Proceedings of the National Academy of Sciences of the United States of America, 93 15
S. Winans (1992)
Two-way chemical signaling in Agrobacterium-plant interactions.Microbiological reviews, 56 1
John Zupan, Patricia Zambryski, V. Citovsky (1997)
The Agrobacterium DNA Transfer ComplexCritical Reviews in Plant Sciences, 16
L. Franzmann, Elizabeth Yoon, D. Meinke (1995)
Saturating the genetic map of Arabidopsis thaliana with embryonic mutationsPlant Journal, 7
J. Masson, P. King, J. Paszkowski (1997)
Mutants of Arabidopsis thaliana hypersensitive to DNA-damaging treatments.Genetics, 146 1
Soma Narasimhulu, Xiao-bing Deng, Rodrigo Sarria, S. Gelvin (1996)
Early transcription of Agrobacterium T-DNA genes in tobacco and maize.The Plant cell, 8 5
Toshiharu Ohba, Y. Yoshioka, C. Machida, Y. Machida (1995)
DNA rearrangement associated with the integration of T-DNA in tobacco: an example for multiple duplications of DNA around the integration target.The Plant journal : for cell and molecular biology, 7 1
E. McKinney, Nazeem Ali, Alice Traut, K. Feldmann, D. Belostotsky, J. McDowell, R. Meagher (1995)
Sequence-based identification of T-DNA insertion mutations in Arabidopsis: actin mutants act2-1 and act4-1.The Plant journal : for cell and molecular biology, 8 4
Yaoguang Liu, N. Mitsukawa, T. Oosumi, R. Whittier (1995)
Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR.The Plant journal : for cell and molecular biology, 8 3
J. Nam, A. Matthysse, S. Gelvin (1997)
Differences in susceptibility of Arabidopsis ecotypes to crown gall disease may result from a deficiency in T-DNA integration.The Plant cell, 9
Ricardo Azpiroz-Leehan, K. Feldmann (1997)
T-DNA insertion mutagenesis in Arabidopsis: going back and forth.Trends in genetics : TIG, 13 4
M. Frey, C. Stettner, A. Gierl (1998)
A general method for gene isolation in tagging approaches: amplification of insertion mutagenised sites (AIMS)Plant Journal, 13
D. Errampalli, D. Patton, Linda Castle, Leigh Mickelson, Karl Hansen, Jennifer Schnall, Kenneth Feldmann, D. Meinke (1991)
Embryonic Lethals and T-DNA Insertional Mutagenesis in Arabidopsis.The Plant cell, 3
E. Howard, V. Citovsky (1990)
The emerging structure of the Agrobacterium T‐DNA transfer complexBioEssays, 12
K. Feldmann (1991)
T‐DNA insertion mutagenesis in Arabidopsis: mutational spectrumPlant Journal, 1
S. Stachel, B. Timmerman, P. Zambryski (1986)
Generation of single-stranded T-DNA molecules during the initial stages of T-DNA transfer from Agrobacterium tumefaciens to plant cellsNature, 322
John Zupan, P. Zambryski (1995)
Transfer of T-DNA from Agrobacterium to the Plant Cell, 107
Jaideep Mathur, László Szabados, S. Schaefer, Britta Grunenberg, Andrea Lossow, Esther Jonas-Straube, Jeff Schell, Csaba Koncz, Z. Koncz-Kálmán (1998)
Gene identification with sequenced T-DNA tags generated by transformation of Arabidopsis cell suspension.The Plant journal : for cell and molecular biology, 13 5
M. Ni, D. Cui, Jane Einstein, S. Narasimhulu, C. Vergara, S. Gelvin (1995)
Strength and tissue specificity of chimeric promoters derived from the octopine and mannopine synthase genesPlant Journal, 7
We have identified T-DNA tagged Arabidopsis mutants that are resistant to transformation by Agrobacterium tumefaciens (rat mutants). These mutants are highly recalcitrant to the induction of both crown gall tumors and phosphinothricin-resistant calli. The results of transient GUS (β-glucuronidase) assays suggest that some of these mutants are blocked at an early step in the Agrobacterium-mediated transformation process, whereas others are blocked at a step subsequent to translocation of T-DNA into the nucleus. Attachment of Agrobacterium to roots of the mutants rat1 and rat3 was decreased under various incubation conditions. In most mutants, the transformation-deficient phenotype co-segregated with the kanamycin resistance encoded by the mutagenizing T-DNA. In crosses with susceptible wild-type plants, the resistance phenotype of many of these mutants segregated either as a semi-dominant or dominant trait.
Molecular Genetics and Genomics – Springer Journals
Published: Apr 1, 1999
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