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Characterization of a Novel Pollen-Specific Promoter from Wheat (Triticum Aestivum L.)

Characterization of a Novel Pollen-Specific Promoter from Wheat (Triticum Aestivum L.) PSG076 is a pollen-specific gene isolated from wheat. The 1.4-kb promoter upstream of the ATG start codon was isolated by inverse-PCR (IPCR). To determine its activity, the PSG076 promoter was fused with the β-glucuronidase (GUS) reporter gene and introduced into tobacco. Histochemical analysis in transgenic tobacco showed that GUS activity was detected in late bicellular pollen grains and increased rapidly in mature pollen. GUS activity was also detected in pollen tubes of transgenic tobacco. No GUS activity was found in other floral and vegetable tissues. These results indicate that the PSG076 promoter directs pollen-specific activity at late stages of pollen development and pollen tube growth. Deletion analysis showed that a 0.4 kb fragment of the promoter was enough to confer pollen-specific expression. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Reporter Springer Journals

Characterization of a Novel Pollen-Specific Promoter from Wheat (Triticum Aestivum L.)

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References (27)

Publisher
Springer Journals
Copyright
Copyright © 2012 by Springer-Verlag
Subject
Life Sciences; Proteomics; Metabolomics; Plant Breeding/Biotechnology; Plant Sciences; Bioinformatics
ISSN
0735-9640
eISSN
1572-9818
DOI
10.1007/s11105-012-0458-y
Publisher site
See Article on Publisher Site

Abstract

PSG076 is a pollen-specific gene isolated from wheat. The 1.4-kb promoter upstream of the ATG start codon was isolated by inverse-PCR (IPCR). To determine its activity, the PSG076 promoter was fused with the β-glucuronidase (GUS) reporter gene and introduced into tobacco. Histochemical analysis in transgenic tobacco showed that GUS activity was detected in late bicellular pollen grains and increased rapidly in mature pollen. GUS activity was also detected in pollen tubes of transgenic tobacco. No GUS activity was found in other floral and vegetable tissues. These results indicate that the PSG076 promoter directs pollen-specific activity at late stages of pollen development and pollen tube growth. Deletion analysis showed that a 0.4 kb fragment of the promoter was enough to confer pollen-specific expression.

Journal

Plant Molecular Biology ReporterSpringer Journals

Published: May 15, 2012

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