Access the full text.
Sign up today, get DeepDyve free for 14 days.
J Stacey, PG Isaac (1994)
Methods in molecular biology—protocols for nucleic acid analysis by nonradioactive probes
RB Horsch, JE Fry, NL Hoffmann, D Eichholtz, SG Rogers, RT Fraley (1985)
A simple and general method for transferring genes into plantsScience, 227
AM Koltunow, J Truettner, KH Cox, M Wallroth, RB Goldberg (1990)
Different temporal and spatial gene expression patterns occur during anther developmentPlant Cell, 2
K Higo, Y Ugawa, M Iwamoto, T Korenaga (1999)
Plant cis-acting regulatory DNA elements (PLACE) database: 1999Nucleic Acids Res, 27
D Twell, J Yamaguchi, S McCormick (1990)
Pollen-specific gene expression in transgenic plants: coordinate regulation of two different tomato gene promoters during microsporogenesisDevelopment, 109
C Potenza, L Aleman, C Sengupta-Gopalan (2004)
Targeting transgene expression in research, agricultural, and environmental applications: promoters used in plant transformationIn Vitro Cell Dev Plant, 40
M Lescot, P Déhais, G Thijs, K Marchal, Y Moreau, Y Peer, P Rouzé, S Rombauts (2002)
PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequencesNucleic Acids Res, 30
K Weterings, J Schrauwen, G Wullems, D Twell (1995)
Functional dissection of the promoter of the pollen-specific gene NTP303 reveals a novel pollen-specific, and conserved cis-regulatory elementPlant J, 8
JI Park, H Hakozaki, M Endo, Y Takada, H Ito, M Uchida, T Okabe, M Watanabe (2006)
Molecular characterization of mature pollen-specific genes encoding novel small cysteine-rich proteins in rice (Oryza sativa L.)Plant Cell Rep, 25
FD Guerrero, L Crossland, GS Smutzer, DA Hamilton, JP Mascarenhas (1990)
Promoter sequences from a maize pollen-specific gene direct tissue-specific transcription in tobaccoMol Gen Genet, 24
R Breathnach, P Chambon (1981)
Organization and expression of eucaryotic split genes coding for proteinsAnnu Rev Biochem, 50
RA Jefferson, TA Kavanagh, MW Bevan (1987)
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plantsEMBO J, 6
P Vergne, I Delvallee, C Dumas (1987)
Rapid assessment of microspore and pollen development stage in wheat and maize using DAPI and membrane permeabilizationStain Technol, 62
DA Hamilton, YH Schwarz, JP Mascarenhas (1998)
A monocot pollen-specific promoter contains separable pollen-specific and quantitative elementsPlant Mol Biol, 38
HJ Rogers, N Bate, J Combe, J Sullivan, J Sweetman, C Swan, DM Lonsdale, D Twell (2001)
Functional analysis of cis-regulatory elements within the promoter of the tobacco late pollen gene g10Plant Mol Biol, 45
SN Covey, GP Lomonossoff, R Hull (1981)
Characterisation of cauliflower mosaic virus DNA sequences which encode major polyadenylated transcriptsNucleic Acids Res, 9
D Twell, J Yamaguchi, RA Wing, J Ushiba, S McCormick (1991)
Promoter analysis of genes that are coordinately expressed during pollen development reveals pollen-specific enhancer sequences and shared regulatory elementsGenes Dev, 5
H Kato, G Xie, Y Sato, R Imai (2010)
Isolation of anther-specific gene promoters suitable for transgene expression in ricePlant Mol Biol Rep, 28
JF Digeon, E Guiderdoni, R Alary, N Michaux-Ferrière, P Joudrier, MF Gautier (1999)
Cloning of a wheat puroindoline gene promoter by IPCR and analysis of promoter regions required for tissue-specific expression in transgenic rice seedsPlant Mol Biol, 39
YF Jin, TF Bian (2004)
Isolation and partial characterization of a novel pollen-specific cDNA with multiple polyadenylation sites from wheatActa Biochim Biophys Sin (Shanghai), 36
N Bate, D Twell (1998)
Functional architecture of a late pollen promoter: pollen-specific transcription is developmentally regulated by multiple stage-specific and co-dependent activator elementsPlant Mol Biol, 37
L Chen, Z Tu, J Hussain, L Cong, Y Yan, L Jin, G Yang, G He (2010)
Isolation and heterologous transformation analysis of a pollen-specific promoter from wheat (Triticum aestivum L.)Mol Biol Rep, 37
MM Bradford (1976)
A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye bindingAnal Biochem, 72
JB Custers, MT Oldenhof, JA Schrauwen, JH Cordewener, GJ Wullems, MM Lookeren Campagne (1997)
Analysis of microspore-specific promoters in transgenic tobaccoPlant Mol Biol, 35
V Gupta, R Khurana, AK Tyagi (2007)
Promoters of two anther-specific genes confer organ-specific gene expression in a stage-specific manner in transgenic systemsPlant Cell Rep, 26
Z Lang, P Zhou, J Yu, G Ao, Q Zhao (2008)
Functional characterization of the pollen-specific SBgLR promoter from potato (Solanum tuberosum L.)Planta, 227
Q Zhu, MI Ordiz, T Dabi, RN Beachy, C Lamb (2002)
Rice TATA binding protein interacts functionally with transcription factor IIB and the RF2a bZIP transcriptional activator in an enhanced plant in vitro transcription systemPlant Cell, 14
PSG076 is a pollen-specific gene isolated from wheat. The 1.4-kb promoter upstream of the ATG start codon was isolated by inverse-PCR (IPCR). To determine its activity, the PSG076 promoter was fused with the β-glucuronidase (GUS) reporter gene and introduced into tobacco. Histochemical analysis in transgenic tobacco showed that GUS activity was detected in late bicellular pollen grains and increased rapidly in mature pollen. GUS activity was also detected in pollen tubes of transgenic tobacco. No GUS activity was found in other floral and vegetable tissues. These results indicate that the PSG076 promoter directs pollen-specific activity at late stages of pollen development and pollen tube growth. Deletion analysis showed that a 0.4 kb fragment of the promoter was enough to confer pollen-specific expression.
Plant Molecular Biology Reporter – Springer Journals
Published: May 15, 2012
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.