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EFFECT OF MICROBIAL GROWTH UPON MYOFIBRILLAR PROTEINS

EFFECT OF MICROBIAL GROWTH UPON MYOFIBRILLAR PROTEINS SUMMARY— Aseptic samples from pig and rabbit muscles were inoculated with Achromobacter liquefaciens, Micrococcus luteus, Pediococcus cerevisiae, Pseudomonas fluorescens, Streptococcus faecal is end a mixed culture obtained from commercial hamburger. Some difficulty was encountered in getting the organisms to grow, and good growth was achieved only with A. liquefaciens end mixed culture from commercial meat. Both inoculated and uninoculated control samples were incubated at 3 and 10°C for 0, 8 and 20 days. The salt soluble proteins were extracted with Weber‐Edsall solution and subjected to sucrose density gradient centrifugation, gel filtration and disc gel electrophoresis. The microorganisms utilized in this study had no measurable effect upon the myofibrillar proteins from either pig or rabbit muscle. However, bacterial growth decreased the amount of certain non‐protein ultra‐violet absorbing components in the ultracentrifugal pattern of Weber‐Edsall extract. These components did not appear to be of myofibrillar origin. Disc gel patterns of Weber‐Edsall extracts from pig muscle produced a more intensely staining band than those from rabbit muscle at Rm, = 0.59. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Food Science Wiley

EFFECT OF MICROBIAL GROWTH UPON MYOFIBRILLAR PROTEINS

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References (11)

Publisher
Wiley
Copyright
Copyright © 1970 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-1147
eISSN
1750-3841
DOI
10.1111/j.1365-2621.1970.tb00971.x
Publisher site
See Article on Publisher Site

Abstract

SUMMARY— Aseptic samples from pig and rabbit muscles were inoculated with Achromobacter liquefaciens, Micrococcus luteus, Pediococcus cerevisiae, Pseudomonas fluorescens, Streptococcus faecal is end a mixed culture obtained from commercial hamburger. Some difficulty was encountered in getting the organisms to grow, and good growth was achieved only with A. liquefaciens end mixed culture from commercial meat. Both inoculated and uninoculated control samples were incubated at 3 and 10°C for 0, 8 and 20 days. The salt soluble proteins were extracted with Weber‐Edsall solution and subjected to sucrose density gradient centrifugation, gel filtration and disc gel electrophoresis. The microorganisms utilized in this study had no measurable effect upon the myofibrillar proteins from either pig or rabbit muscle. However, bacterial growth decreased the amount of certain non‐protein ultra‐violet absorbing components in the ultracentrifugal pattern of Weber‐Edsall extract. These components did not appear to be of myofibrillar origin. Disc gel patterns of Weber‐Edsall extracts from pig muscle produced a more intensely staining band than those from rabbit muscle at Rm, = 0.59.

Journal

Journal of Food ScienceWiley

Published: Jul 1, 1970

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