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References (9)
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Cytotoxicity test for the typing of human lymphocytes. A modified micromethod.Technical bulletin of the Registry of Medical Technologists. American Society of Clinical Pathologists. Registry of Medical Technologists, 39 3
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Reprinted from Technical Bulletin ofthe Registry of Medical Technologists, 39, 3 . VAN ROOD -
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Troup Troup, Walford Walford (1969)
Cytotoxicity test for the typing of human lymphocytes. A modified methodAmerican Journal of Clinical Pathology, 51
- Publisher
- Wiley
- Copyright
- Copyright © 1970 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 0007-1048
- eISSN
- 1365-2141
- DOI
- 10.1111/j.1365-2141.1970.tb01436.x
- Publisher site
- See Article on Publisher Site
Abstract
Summary. A reliable rapid one‐step method is described for the preparation of lymphocytes from 0.25 ml aliquots of defibrinated whole venous blood for tissue typing. An average yield of 70% lymphocytes is obtained relatively free from platelets, erythrocytes and granulocytes even in the presence of large numbers of granulocytes in blood obtained from cadaver donors. Using a high‐speed micro‐centrifuge lymphocytes can be prepared from defibrinated blood within 10 min and it is thus possible to complete a tissue typing test within 75 min. Using this method sufficient lymphocytes for typing purposes can be obtained from 0.5 ml of capillary blood thus greatly facilitating family studies where infants are involved.
Journal
British Journal of Haematology – Wiley
Published: Feb 1, 1970