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L. Vindeløv, H. Hansen, I. Christensen, M. Spang‐Thomsen, F. Hirsch, M. Hansen, N. Nissen (1980)
Clonal heterogeneity of small-cell anaplastic carcinoma of the lung demonstrated by flow-cytometric DNA analysis.Cancer research, 40 11
J. Lepecq, C. Paoletti (1967)
A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.Journal of molecular biology, 27 1
HA Crissman, RA Tobey (1974)
Cell‐cycle analysis in 20 min, 184
H. Crissman, R. Tobey (1974)
Cell-Cycle Analysis in 20 MinutesScience, 184
L. Vindeløv, I. Christensen, N. Nissen (1983)
Standardization of high-resolution flow cytometric DNA analysis by the simultaneous use of chicken and trout red blood cells as internal reference standards.Cytometry, 3 5
L. Vindeløv, I. Christensen, Niels Keiding, M. Spang‐Thomsen, N. Nissen (1983)
Long-term storage of samples for flow cytometric DNA analysis.Cytometry, 3 5
J Gaub (1976)
Feulgen‐naphthol yellow S cytophotometry of liver cells, 49
L. Vindeløv, H. Hansen, A. Gersel, F. Hirsch, N. Nissen (1982)
Treatment of small-cell carcinoma of the lung monitored by sequential flow cytometric DNA analysis.Cancer research, 42 6
L. Vindeløv, I. Christensen, G. Jensen, N. Nissen (1983)
Limits of detection of nuclear DNA abnormalities by flow cytometric DNA analysis. Results obtained by a set of methods for sample-storage, staining and internal standardization.Cytometry, 3 5
JB LePecq, C Paoletti (1967)
A fluorescent complex between ethidium bromide and nucleic acids, 27
H. Busch, K. Narayan, J. Hamilton (1967)
Isolation of nucleoli in a medium containing spermine and magnesium acetate.Experimental cell research, 47 1
LL Vindeløv (1977)
Flow microfluorometric analysis of nuclear DNA in cells from solid tumors and cell suspensions, 24
J Zante, J Schumann, B Barlogie, W Göhde, T Büchner (1976)
Pulse‐Cytophotometry, 2
Anton Martens, Gerrit Engh, Anton Hagenbeek (1981)
The fluorescence intensity of propidium iodide bound to DNA depends on the concentration of sodium chloride.Cytometry, 2 1
M. BICHEL, P. Frederiksen, D. T.KJERMDP.THOMMESENM, MD VINDELV, Mrs. Kierkegaard, Miss Krarup, Mrs. Spliid, Mrs. Wagner (1977)
Flow microfluorometry and transrectal fineneedle biopsy in the classification of human prostatic carcinomaCancer, 40
A. Bøyum (2008)
Separation of blood leucocytes, granulocytes and lymphocytes.Tissue antigens, 4 4
A. Krishan (1975)
Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide stainingThe Journal of Cell Biology, 66
A new modification of our detergent technique for the preparation of nuclei for flow cytometric DNA analysis is described. The attainment of low coefficients of variation of the peaks and of quantitative staining of nuclei from different tissues was a problem with the original method. This was solved in the new modification by trypsinization of the unfixed nuclei. The nuclei were stabilized by spermine. A simple procedure for long‐term storage of samples at —80°C was integrated into the method. The fluorescence of the nuclei was stable for at least 3 hours after staining. Light exposure protection of the samples was essential. No cell loss was caused by storage or staining. The method was successfully applied on samples including: (a) Normal tissues— human lymphocytes, granulocytes and spleen. Mouse lymphocytes, bone marrow, spleen, liver, kidney and thymus. (b) Human neoplasms— lung cancer, breast cancer, lymphoma, leukemia, bladder cancer and cancer of the oral cavity. (c) Human tumors in nude mice— breast cancer, lung cancer, melanoma and colon cancer. (d) Mouse ascites tumors— JB‐1, L 1210, Ehrlich and P 383. It therefore seems well suited as a routine clinical procedure.
Cytometry Part A – Wiley
Published: Mar 1, 1983
Keywords: ; ; ; ; ;
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