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In Vivo Measurement of Dopamine and Its Metabolites by Intracerebral Dialysis: Changes After d ‐Amphetamine

In Vivo Measurement of Dopamine and Its Metabolites by Intracerebral Dialysis: Changes After d... Abstract: By using a new technique, intracerebral dialysis, in combination with high performance liquid chromatography and electrochemical detection, it was possible to recover and measure endogenous extracellular dopamine, together with its metabolites dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) from the striatum and nucleus accumbens of anaesthetized or freely moving rats. In addition, measurements of extracellular 5‐hydroxyindoleacetic acid, ascorbic acid, and uric acid were made. Basal extracellular concentrations of dopamine and DOPAC in the striatum were estimated to be 5 × 10−8M and 5 × 10−6M, respectively. d‐Amphetamine (2 mg/kg s.c.) increased dopamine levels in the striatum perfusates by 14‐fold, whereas levels of DOPAC and HVA decreased by 77% and 66%, respectively. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

In Vivo Measurement of Dopamine and Its Metabolites by Intracerebral Dialysis: Changes After d ‐Amphetamine

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References (29)

Publisher
Wiley
Copyright
Copyright © 1983 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1983.tb00893.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: By using a new technique, intracerebral dialysis, in combination with high performance liquid chromatography and electrochemical detection, it was possible to recover and measure endogenous extracellular dopamine, together with its metabolites dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) from the striatum and nucleus accumbens of anaesthetized or freely moving rats. In addition, measurements of extracellular 5‐hydroxyindoleacetic acid, ascorbic acid, and uric acid were made. Basal extracellular concentrations of dopamine and DOPAC in the striatum were estimated to be 5 × 10−8M and 5 × 10−6M, respectively. d‐Amphetamine (2 mg/kg s.c.) increased dopamine levels in the striatum perfusates by 14‐fold, whereas levels of DOPAC and HVA decreased by 77% and 66%, respectively.

Journal

Journal of NeurochemistryWiley

Published: Jun 1, 1983

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