Access the full text.
Sign up today, get DeepDyve free for 14 days.
M. Weissman, H. Schindler, G. Feher (1976)
Determination of molecular weights by fluctuation spectroscopy: application to DNA.Proceedings of the National Academy of Sciences of the United States of America, 73 8
E. Elson, W. Webb (1975)
Concentration correlation spectroscopy: a new biophysical probe based on occupation number fluctuations.Annual review of biophysics and bioengineering, 4 00
P. Fahey, D. Koppel, L. Barak, D. Wolf, E. Elson, W. Webb (1977)
Lateral diffusion in planar lipid bilayers.Science, 195 4275
D. Koppel, D. Axelrod, J. Schlessinger, Elliot Elson, W. Webb (1976)
Dynamics of fluorescence marker concentration as a probe of mobility.Biophysical journal, 16 11
J. Yardley, L. Specht (1976)
Orientational relaxation by fluorescence correlationChemical Physics Letters, 37
D. Magde, E. Elson, W. Webb (1972)
Thermodynamic Fluctuations in a Reacting System-Measurement by Fluorescence Correlation SpectroscopyPhysical Review Letters, 29
D. Magde (1977)
Concentration correlation analysis and chemical kinetics.Molecular biology, biochemistry, and biophysics, 24
R. Voss, J. Clarke (1976)
Number fluctuation spectra from light scattered by particles in Brownian motionJournal of Physics A, 9
E. Elson, D. Magde (1974)
Fluorescence correlation spectroscopy. I. Conceptual basis and theoryBiopolymers, 13
S. Aragon, R. Pecora (1976)
Fluorescence correlation spectroscopy as a probe of molecular dynamicsJournal of Chemical Physics, 64
B. Berne, R. Pecora (1976)
Dynamic Light Scattering: With Applications to Chemistry, Biology, and Physics
S. Aragon, R. Pecora (1975)
Fluorescence correlation spectroscopy and Brownian rotational diffusionBiopolymers, 14
D. Magde, E. Elson, W. Webb (1974)
Fluorescence correlation spectroscopy. II. An experimental realizationBiopolymers, 13
D. Schaefer (1973)
Dynamics of Number Fluctuations: Motile MicroorganismsScience, 180
M. Ehrenberg, R. Rigler (1974)
Rotational brownian motion and fluorescence intensify fluctuations, 4
I. Pecht, R. Rigler (1977)
Chemical Relaxation in Molecular Biology
D. Koppel (1974)
Statistical accuracy in fluorescence correlation spectroscopyPhysical Review A, 10
We extend fluorescence correlation spectroscopy to systems that undergo translation or laminar flow in a sample cell. We include theoretical and experimental results; we consider uniform and nonuniform velocity profiles. Concentration correlation analysis extracts microscopic rate parameters from measurements of the spontaneous concentration fluctutations, which occur even at equilibrium. Fluorescence is one of the most sensitive means of monitoring these fluctuations. Analysis of flowing or translating systems (1) offers a method of measuring number concentrations of selected species, for example, of aggregates or polymers, (2) provides a nonperturbing velocity probe, (3) sometimes allows one to circumvent photolytic degradation, (4) has proved extremely helpful in testing and aliging apparatus for fluorescence correaltion measurement and in verifying theoretical analyses, and (5) may be required for interpretation of results obtained on systems in motion, even though that motion is undesired or initially unsuspected. We include both theoretical and experimental results for combined Poiseuille flow and diffusion in the geometry which is of most practical interest. Theoretical expressions for the much simpler cases of nondiffusive Poiseuille flow as well as uniform flow or translation with or without diffusion constitute limiting cases which are displayed explicitly.
Biopolymers – Wiley
Published: Feb 1, 1978
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.