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Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants

Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic... To improve the accumulation of recombinant human epidermal growth factor (hEGF) in transgenic tobacco, a highly effective vector was constructed and transformed via Agrobacterium tumefaciens. The hEGF content in transgenic tobacco was up to 0.3% of the total soluble protein. Using the Vero E6 cell expansion assay and the MTT method for cell proliferation, hEGF produced by transgenic tobacco significantly stimulated Vero E6 cell expansion and proliferation, the same as commercial hEGF products. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology Letters Springer Journals

Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants

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References (20)

Publisher
Springer Journals
Copyright
Copyright © 2007 by Springer Science+Business Media B.V.
Subject
Life Sciences; Biochemistry, general; Applied Microbiology; Biotechnology; Microbiology
ISSN
0141-5492
eISSN
1573-6776
DOI
10.1007/s10529-007-9438-y
pmid
17896082
Publisher site
See Article on Publisher Site

Abstract

To improve the accumulation of recombinant human epidermal growth factor (hEGF) in transgenic tobacco, a highly effective vector was constructed and transformed via Agrobacterium tumefaciens. The hEGF content in transgenic tobacco was up to 0.3% of the total soluble protein. Using the Vero E6 cell expansion assay and the MTT method for cell proliferation, hEGF produced by transgenic tobacco significantly stimulated Vero E6 cell expansion and proliferation, the same as commercial hEGF products.

Journal

Biotechnology LettersSpringer Journals

Published: Sep 25, 2007

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