Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Long‐term potentiation in the nucleus accumbens requires both NR2A‐ and NR2B‐containing N ‐methyl‐ d ‐aspartate receptors

Long‐term potentiation in the nucleus accumbens requires both NR2A‐ and NR2B‐containing N... N‐methyl‐d‐aspartate (NMDA) receptors play crucial roles in several forms of long‐term changes in the efficacy of glutamatergic synaptic transmission. The suggestion that the NR2A subunit of the NMDA receptor may be selectively involved in the induction of long‐term potentiation (LTP) in the hippocampus and cortex has been challenged. However, the contribution of NR2B in the induction of LTP is not always clearly established. The present study investigates the role of NR2A and NR2B in the induction of LTP in the nucleus accumbens (NAc), a brain region that expresses high levels of NR2B and an NMDA‐dependent form of LTP. We recorded extracellular field excitatory postsynaptic potentials/population spikes in slices of mouse NAc. High‐frequency stimulation of glutamatergic fibers consistently induced LTP of the field excitatory postsynaptic potential/population spike in the NAc. LTP was abolished in the presence of selective antagonists of either NR2B (R‐(R*,S*)‐α‐(4‐hydroxyphenyl)‐β‐methyl‐4‐(phenyl‐methyl)‐1‐piperidine propanol and Ifenprodil) or NR2A (((R)‐((S)‐1‐(4‐bromo‐phenyl)‐ethylamino)‐(2,3‐dioxo‐1,2,3,4‐tetrahydroquinoxalin‐5‐yl)‐methyl)‐phosphonic acid) subunits. Recordings performed in a low concentration of Mg2+ ions in the perfusion solution did not reveal a selective involvement of a particular NMDA receptor subunit because either NR2A or NR2B antagonists were able to block LTP. LTP was also abolished in the presence of a low concentration of the non‐subunit‐selective NMDA receptor antagonist dl‐2‐amino‐5‐phosphonopentanoic acid in normal Mg2+ and low Mg2+ in the perfusion solution. These results show that the degree of NMDA receptor activation, and not their subunit composition, determines whether LTP is induced in the NAc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Neuroscience Wiley

Long‐term potentiation in the nucleus accumbens requires both NR2A‐ and NR2B‐containing N ‐methyl‐ d ‐aspartate receptors

Loading next page...
 
/lp/wiley/long-term-potentiation-in-the-nucleus-accumbens-requires-both-nr2a-and-EDWsiwiL83

References (50)

Publisher
Wiley
Copyright
© The Authors (2008). Journal Compilation © Federation of European Neuroscience Societies and Blackwell Publishing Ltd
ISSN
0953-816X
eISSN
1460-9568
DOI
10.1111/j.1460-9568.2008.06173.x
pmid
18412616
Publisher site
See Article on Publisher Site

Abstract

N‐methyl‐d‐aspartate (NMDA) receptors play crucial roles in several forms of long‐term changes in the efficacy of glutamatergic synaptic transmission. The suggestion that the NR2A subunit of the NMDA receptor may be selectively involved in the induction of long‐term potentiation (LTP) in the hippocampus and cortex has been challenged. However, the contribution of NR2B in the induction of LTP is not always clearly established. The present study investigates the role of NR2A and NR2B in the induction of LTP in the nucleus accumbens (NAc), a brain region that expresses high levels of NR2B and an NMDA‐dependent form of LTP. We recorded extracellular field excitatory postsynaptic potentials/population spikes in slices of mouse NAc. High‐frequency stimulation of glutamatergic fibers consistently induced LTP of the field excitatory postsynaptic potential/population spike in the NAc. LTP was abolished in the presence of selective antagonists of either NR2B (R‐(R*,S*)‐α‐(4‐hydroxyphenyl)‐β‐methyl‐4‐(phenyl‐methyl)‐1‐piperidine propanol and Ifenprodil) or NR2A (((R)‐((S)‐1‐(4‐bromo‐phenyl)‐ethylamino)‐(2,3‐dioxo‐1,2,3,4‐tetrahydroquinoxalin‐5‐yl)‐methyl)‐phosphonic acid) subunits. Recordings performed in a low concentration of Mg2+ ions in the perfusion solution did not reveal a selective involvement of a particular NMDA receptor subunit because either NR2A or NR2B antagonists were able to block LTP. LTP was also abolished in the presence of a low concentration of the non‐subunit‐selective NMDA receptor antagonist dl‐2‐amino‐5‐phosphonopentanoic acid in normal Mg2+ and low Mg2+ in the perfusion solution. These results show that the degree of NMDA receptor activation, and not their subunit composition, determines whether LTP is induced in the NAc.

Journal

European Journal of NeuroscienceWiley

Published: Apr 1, 2008

There are no references for this article.