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Antoine Martin, Judy Lee, Thomas Kichey, Denise Gerentes, M. Zivy, C. Tatout, F. Dubois, Thierry Balliau, B. Valot, Marlène Davanture, T. Tercé-laforgue, I. Quilleré, M. Coque, A. Gallais, M. González-Moro, L. Béthencourt, D. Habash, P. Lea, A. Charcosset, P. Perez, A. Murigneux, H. Sakakibara, K. Edwards, B. Hirel (2006)
Two Cytosolic Glutamine Synthetase Isoforms of Maize Are Specifically Involved in the Control of Grain Production[W][OA]The Plant Cell Online, 18
H. White, V. Hall, N. Cross (2007)
Methylation-sensitive high-resolution melting-curve analysis of the SNRPN gene as a diagnostic screen for Prader-Willi and Angelman syndromes.Clinical chemistry, 53 11
J. Ihrig, R. Lill, U. Mühlenhoff (2006)
Application of the DNA-specific dye EvaGreen for the routine quantification of DNA in microplates.Analytical biochemistry, 359 2
E. Greene, Christine Codomo, Nicholas Taylor, J. Henikoff, B. Till, S. Reynolds, L. Enns, C. Burtner, Jessica Johnson, Anthony Odden, L. Comai, S. Henikoff (2003)
Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis.Genetics, 164 2
Hua Sun, Hong Wang, W. Kwon, J. In, Bum-Soo Lee, Deok-Chun Yang (2010)
Development of molecular markers for the determination of the new cultivar 'Chunpoong' in Panax ginseng C. A. Meyer associated with a major latex-like protein gene.Biological & pharmaceutical bulletin, 33 2
B. Hofinger, Hai-Chun Jing, K. Hammond-Kosack, K. Kanyuka (2009)
High-resolution melting analysis of cDNA-derived PCR amplicons for rapid and cost-effective identification of novel alleles in barleyTheoretical and Applied Genetics, 119
H. Carén, A. Holmstrand, R. Sjöberg, T. Martinsson (2006)
The two human homologues of yeast UFD2 ubiquitination factor, UBE4A and UBE4B, are located in common neuroblastoma deletion regions and are subject to mutations in tumours.European journal of cancer, 42 3
M. Herrmann, J. Durtschi, L. Bromley, C. Wittwer, C. Wittwer, K. Voelkerding, K. Voelkerding (2006)
Amplicon DNA melting analysis for mutation scanning and genotyping: cross-platform comparison of instruments and dyes.Clinical chemistry, 52 3
H. Akiyama, Fumi Nakamura, C. Yamada, Kosuke Nakamura, O. Nakajima, H. Kawakami, N. Harikai, S. Furui, K. Kitta, R. Teshima (2009)
A screening method for the detection of the 35S promoter and the nopaline synthase terminator in genetically modified organisms in a real-time multiplex polymerase chain reaction using high-resolution melting-curve analysis.Biological & pharmaceutical bulletin, 32 11
Weijie Wang, Kun-song Chen, Changjie Xu (2006)
DNA quantification using EvaGreen and a real-time PCR instrument.Analytical biochemistry, 356 2
Fuming Sang, Jicun Ren (2006)
Capillary electrophoresis of double-stranded DNA fragments using a new fluorescence intercalating dye EvaGreen.Journal of separation science, 29 9
T. Ujino-Ihara, Yuriko Taguchi, Y. Moriguchi, Y. Tsumura (2010)
An efficient method for developing SNP markers based on EST data combined with high resolution melting (HRM) analysisBMC Research Notes, 3
Vinod Dagar, C. Chow, D. Ashley, E. Algar (2009)
Rapid detection of SMARCB1 sequence variation using high resolution meltingBMC Cancer, 9
A. Croxford, T. Rogers, P. Caligari, Michael Wilkinson (2008)
High-resolution melt analysis to identify and map sequence-tagged site anchor points onto linkage maps: a white lupin (Lupinus albus) map as an exemplar.The New phytologist, 180 3
Shu‐Biao Wu, M. Wirthensohn, P. Hunt, J. Gibson, M. Sedgley (2008)
High resolution melting analysis of almond SNPs derived from ESTsTheoretical and Applied Genetics, 118
C. Wittwer, G. Reed, C. Gundry, J. Vandersteen, R. Pryor (2003)
High-resolution genotyping by amplicon melting analysis using LCGreen.Clinical chemistry, 49 6 Pt 1
R. Okagaki, M. Neuffer, S. Wessler (1991)
A deletion common to two independently derived waxy mutations of maize.Genetics, 128 2
High‐resolution melting (HRM) analysis relies on the use of fluorescent dyes, such as LCGreen, ResoLight, and SYTO9, which bind in a saturated manner to the double‐stranded DNAs. These dyes are expensive in use and may not be affordable when dealing with a large quantity of samples. EvaGreen is a much cheaper DNA helix intercalating dye and has been used in quantitative real‐time polymerase chain reaction (PCR) and post‐PCR DNA melt curve analysis. Here we report on the development of an EvaGreen‐based HRM analysis and its performance, in comparison with the popular LCGreen‐based HRM analysis, in detection of DNA polymorphism in plants. We found that various polymorphisms ranged from single nucleotide polymorphisms (SNPs) to Indels were equally detected by using EvaGreen‐ or LCGreen‐based HRM. EvaGreen dye was sensitive enough in discovery of SNPs in fivefold pooled samples. Using this economical dye we successfully identified multiple novel mutant alleles of Gln1‐3 gene, which produces a cytosolic glutamine synthetase isoenzyme (GS1), in a maize ethyl methanesulfonate (EMS)‐mutagenized library, and genotyped rice mapping populations with SNP markers. The current results suggest that EvaGreen is a promising dye for HRM analysis for its ease to use and cost effectiveness.
Journal of Integrative Plant Biology – Wiley
Published: Dec 1, 2010
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