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B. Juurlink, A. Schousboe, O. Jørgensen, L. Hertz (1981)
Induction by Hydrocortisone of Glutamine Synthetase in Mouse Primary Astrocyte CulturesJournal of Neurochemistry, 36
O. Lowry, N. Rosebrough, A. Farr, R. Randall (1951)
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R. Lim, D. Turriff, S. Troy (1976)
Response of glioblasts to a morphological transforming factor: cinematographic and chemical correlationsBrain Research, 113
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Mitogens for glial cells: a comparison of the response of cultured astrocytes, oligodendrocytes and Schwann cells.Brain research, 254 1
H. Thoenen, Y. Barde (1980)
Physiology of nerve growth factor.Physiological reviews, 60 4
B. Pettmann, G. Labourdette, G. Devilliers, M. Sensenbrenner (1981)
Effects of brain extracts from chick embryo on the development of astroblasts in culture.Developmental neuroscience, 4 1
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Growth hormone and related peptides
M. Manthorpe, S. Skaper, R. Adler, K. Landa, S. Varon (1980)
Cholinergic Neuronotrophic Factors: Fractionation Properties of an Extract from Selected Chick Embryonic Eye TissuesJournal of Neurochemistry, 34
Brunner Brunner, Lang Lang, Wolfe Wolfe, McClure McClure, Sato Sato (1982)
Selective cell culture of brain cells by serum‐free hormone‐supplemented media: A comparative morphological studyDev Brain Res, 2
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Stimulation of division of sparse and confluent 3T3 cell populations by a fibroblast growth factor, dexamethasone, and insulin.Proceedings of the National Academy of Sciences of the United States of America, 71 11
Gérard Labourdette, Alexander Marks (1975)
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B. Pettmann, M. Sensenbrenner, G. Labourdette (1980)
Isolation of a glial maturation factor from beef brainFEBS Letters, 118
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Regulation of glutamine synthetase in cultured 3T3-L1 cells by insulin, hydrocortisone, and dibutyryl cyclic AMP.Proceedings of the National Academy of Sciences of the United States of America, 75 3
A. Schousboe, G. Svenneby, L. Hertz (1977)
UPTAKE AND METABOLISM OF GLUTAMATE IN ASTROCYTES CULTURED FROM DISSOCIATED MOUSE BRAIN HEMISPHERESJournal of Neurochemistry, 29
Organ Cultures in Neurobiology
K. Hallermayer, C. Harmening, B. Hamprecht (1981)
Cellular Localization and Regulation of Glutamine Synthetase in Primary Cultures of Brain Cells from Newborn MiceJournal of Neurochemistry, 37
G. Brunner, K. Lang, R. Wolfe, D. Mcclure, G. Sato (1981)
Selective cell culture of brain cells by serum-free, hormone-supplemented media: a comparative morphological study.Brain research, 254 4
Y. Shimizu, N. Shimizu (1981)
Insulin and epidermal growth factor stimulate poly ADP-ribosylation.Biochemical and biophysical research communications, 99 2
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Distribution of glutamine synthetase in the rat central nervous system.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 27
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Chi-Sun Wang, Robert Smith (1975)
Lowry determination of protein in the presence of Triton X-100.Analytical biochemistry, 63 2
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Glia maturation factor in bovine brain: Partial purification and physicochemical characterizationBrain Research, 212
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An improved fluorometric assay for DNA.Analytical biochemistry, 39 1
Pruss Pruss, Bartlett Bartlett, Gavrilovic Gavrilovic, Lisak Lisak, Rattray Rattray (1982)
Mitogens for glial cells: A comparison of the response of cultured astrocytes, oligodendrocytes and Schwann cellsDev Brain Res, 2
J. Booher, M. Sensenbrenner (1972)
Growth and cultivation of dissociated neurons and glial cells from embryonic chick, rat and human brain in flask cultures.Neurobiology, 2 3
M. Sensenbrenner (1977)
DISSOCIATED BRAIN CELLS IN PRIMARY CULTURES
R. Lim (1980)
Glia maturation factor.Current topics in developmental biology, 16
Astroblasts from brain hemispheres of newborn rats were cultivated for 5 days in a complete medium containing 10% fetal calf serum. Cultures were then grown in basal medium in absence of serum. In these conditions, after 15 days only a few cells remained. When the basal culture medium was supplemented with insulin and transferrin (IT medium) the number of the remaining cells was twice higher. However, it was still lower than that found in the 5‐day cultures before serum was removed. Thus the IT medium does not allow a good survival of the cells. Addition to the medium of an astroglial growth factor (AGF) from bovine brain soluble extract led to a tenfold increase of the cell number compared to cultures maintained in the basal medium alone. Effects of the factor on cell survival and on proliferation were examined independently. It was found that the factor did not greatly increase the survival of the cells but that it stimulated the proliferation. Addition of the growth factor to the IT medium elicited an increase of the levels of S‐100 protein and of the activity of glutamine synthetase, two proteins which are specific to astroglial cells in the central nervous system. These results indicate that the astroglial brain factor allows growth and maturation of the rat astroblasts maintained in serum‐free medium.
Journal of Neuroscience Research – Wiley
Published: Jan 1, 1982
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