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References (24)
-
Lin Liang-binb (2012)
Reform Researches on the Comprehensive Experimental Teaching for Molecular Biology -
H. F. Zhang, Gao, Huang, Sheng, Xu, Xu (2009)
Cloning, expression and polyclonal antibody preparation of flagellin gene fljB: z66 of Salmonella enterica serovar Typhi, 19
-
Zhu Yi (2011)
Practice and discussion of comprehensive experiments teaching of molecular biologyJournal of Biology
-
Huang Xin-xiang (2005)
Construction of a fljA Like Gene Deleted Mutant in Salmonella Enterica Serovar TyphiJournal of Zhenjiang Medical College
-
D. L. Nelson, M. M. Cox (2005)
Lehninger Principles of Biochemistry -
S. Baker, K. Holt, S. Whitehead, I. Goodhead, Timothy Perkins, B. Stocker, J. Hardy, G. Dougan (2007)
A linear plasmid truncation induces unidirectional flagellar phase change in H:z66 positive Salmonella TyphiMolecular Microbiology, 66
-
F. Neidhart (1996)
Escherichia coli and Salmonella. -
P. Guiñee, W. Jansen, H. Maas, L. Minor, R. Beaud (1981)
An unusual H antigen (Z66) in strains of Salmonella typhi.Annales de microbiologie, 132 3
-
Ni Yu (2012)
Design and Practice of an Integrated Experiment for Molecular BiologyJournal of Southwest China Normal University
-
P. Edwards, W. Ewing (1986)
Edwards and Ewing's Identification of Enterobacteriaceae -
Xin-xiang Huang, L. Phủng, S. Dejsirilert, P. Tishyadhigama, Y. Li, Hongshen Liu, K. Hirose, Y. Kawamura, T. Ezaki (2004)
Cloning and characterization of the gene encoding the z66 antigen of Salmonella enterica serovar Typhi.FEMS microbiology letters, 234 2
-
Xin Zou, Xin-xiang Huang, Shungao Xu, Liping Zhou, Xiumei Sheng, Haifang Zhang, Huaxi Xu, T. Ezaki (2009)
Identification of a fljA gene on a linear plasmid as the repressor gene of fliC in Salmonella enterica serovar Typhi.Microbiology and immunology, 53 4
-
S. Baker, J. Hardy, K. Sanderson, M. Quail, I. Goodhead, R. Kingsley, J. Parkhill, B. Stocker, G. Dougan (2007)
A Novel Linear Plasmid Mediates Flagellar Variation in Salmonella TyphiPLoS Pathogens, 3
-
I. Henderson, P. Owen, J. Nataro (1999)
Molecular switches — the ON and OFF of bacterial phase variationMolecular Microbiology, 33
-
Ji-cheng Pan (2012)
Research and practice on the construction of national excellent course of biological comprehensive experimentsJournal of Hubei Normal University
-
(2012)
Development of designing biochemistry experiment about the basic course in physical education -
J. M. Berg, J. L. Tymoczko, L. Stryer (2002)
Biochemistry -
K. Tamura, R. Sakazaki, S. Kuramochi, A. Nakamura (1988)
Occurrence of H-antigen z66 of R phase in cultures of Salmonella serovar typhi originated from IndonesiaEpidemiology and Infection, 101
-
R. M. Macnab, Flagella, Motility, F. C. Neidhardt, R. Curtiss, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, H. E. Umbarger (1996)
Escherichia coli and Salmonella: Cellular and Molecular Biology -
Shouji Yamamoto, K. Kutsukake (2006)
FljA-Mediated Posttranscriptional Control of Phase 1 Flagellin Expression in Flagellar Phase Variation of Salmonella enterica Serovar TyphimuriumJournal of Bacteriology, 188
-
F. Asten, H. Hendriks, J. Koninkx, J. Dijk (2004)
Flagella-mediated bacterial motility accelerates but is not required for Salmonella serotype Enteritidis invasion of differentiated Caco-2 cells.International journal of medical microbiology : IJMM, 294 6
-
Zhang Xiang-ming (2011)
New Mode of Modulization Experimental Teaching for Molecular Biology and Gene EngineerExperiment Science and Technology
-
R. Macnab (1996)
Flagella and motility -
Xin-xiang Huang, Huaxi Xu, M. Sha, Licheng Zhao, K. Ohkusu, Y. Kawamura, T. Ezaki (2006)
Virulence‐Defective Strains of Salmonella enterica Serovar Typhi as Candidates for Education at Level 2 FacilitiesMicrobiology and Immunology, 50
- Publisher
- Wiley
- Copyright
- Copyright © 2014 by The International Union of Biochemistry and Molecular Biology
- ISSN
- 1470-8175
- eISSN
- 1539-3429
- DOI
- 10.1002/bmb.20772
- pmid
- 24535961
- Publisher site
- See Article on Publisher Site
Abstract
Scientific experiments are indispensable parts of Biochemistry and Molecular Biology. In this study, a comprehensive Biochemistry and Molecular Biology experiment about Salmonella enterica serovar Typhi Flagellar phase variation has been designed. It consisted of three parts, namely, inducement of bacterial Flagellar phase variation, antibody agglutination test, and PCR analysis. Phase variation was observed by baterial motility assay and identified by antibody agglutination test and PCR analysis. This comprehensive experiment can be performed to help students improve their ability to use the knowledge acquired in Biochemistry and Molecular Biology. © 2014 by The International Union of Biochemistry and Molecular Biology, 42(3):XXXXX–XXXXX, 2014.
Journal
Biochemistry and Molecular Biology Education – Wiley
Published: May 1, 2014
Keywords: ; ; ;