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Differential Expression of Two Genes for Sucrose-Phosphate Synthase in Sugarcane: Molecular Cloning of the cDNAs and Comparative Analysis of Gene Expression

Differential Expression of Two Genes for Sucrose-Phosphate Synthase in Sugarcane: Molecular... Abstract Two cDNA clones, pSoSPSl and pSoSPS2, encoding sucrose-phosphate synthase (SPS) of sugarcane were isolated from a leaf cDNA library. Northern analysis revealed the transcript of SoSPSl to be predominant in leaves, but that of SoSPS2 to be distributed not only in leaves but also in roots at a similar level. The transcript of SoSPSl was markedly accumulated during greening of etiolated leaves, whereas that of SoSPS2 was constitutively expressed. These findings indicate that SPS in sugarcane is encoded by multiple genes, which show organ-specificity and are differentially regulated in response to light. © JSPP http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant and Cell Physiology Oxford University Press

Differential Expression of Two Genes for Sucrose-Phosphate Synthase in Sugarcane: Molecular Cloning of the cDNAs and Comparative Analysis of Gene Expression

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References (15)

Publisher
Oxford University Press
Copyright
© JSPP
ISSN
0032-0781
eISSN
1471-9053
DOI
10.1093/oxfordjournals.pcp.a029258
Publisher site
See Article on Publisher Site

Abstract

Abstract Two cDNA clones, pSoSPSl and pSoSPS2, encoding sucrose-phosphate synthase (SPS) of sugarcane were isolated from a leaf cDNA library. Northern analysis revealed the transcript of SoSPSl to be predominant in leaves, but that of SoSPS2 to be distributed not only in leaves but also in roots at a similar level. The transcript of SoSPSl was markedly accumulated during greening of etiolated leaves, whereas that of SoSPS2 was constitutively expressed. These findings indicate that SPS in sugarcane is encoded by multiple genes, which show organ-specificity and are differentially regulated in response to light. © JSPP

Journal

Plant and Cell PhysiologyOxford University Press

Published: Jan 1, 1997

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