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Agrobacterium tumefaciens -mediated transformation of Festuca arundinacea (Schreb.) and Lolium multiflorum (Lam.)

Agrobacterium tumefaciens -mediated transformation of Festuca arundinacea (Schreb.) and Lolium... Agrobacterium tumefaciens strain LBA4404 carrying plasmid pTOK233 encoding the hygromycin resistance ( hph ) and β-glucuronidase ( uidA ) genes has been used to transform two agronomic grass species: tall fescue (Festuca arundinacea ) and Italian ryegrass ( Lolium multiflorum ). Embryogenic cell suspension colonies or young embryogenic calli were co-cultured with Agrobacterium in the presence of acetosyringone. Colonies were grown under hygromycin selection with cefotaxime and surviving colonies plated on embryogenesis media. Eight Lolium (six independent lines) and two Festuca plants (independent lines) were regenerated and established in soil. All plants were hygromycin-resistant, but histochemical determination of GUS activity showed that only one Festuca plant and one Lolium plant expressed GUS. Three GUS-negative transgenic L. multiflorum and the two F. arundinacea plants were vernalised and allowed to flower. All three Lolium plants were male- and female-fertile, but the Festuca plants failed to produce seed. Progeny analysis of L. multiflorum showed a 24–68% inheritance of the hph and uidA genes in the three lines with no significant difference between paternal and maternal gene transmission. However, significant differences were noted between the paternal and maternal expression of hygromycin resistance. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

Agrobacterium tumefaciens -mediated transformation of Festuca arundinacea (Schreb.) and Lolium multiflorum (Lam.)

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References (25)

Publisher
Springer Journals
Copyright
Copyright © 2003 by Springer-Verlag
Subject
Legacy
ISSN
0721-7714
eISSN
1432-203X
DOI
10.1007/s00299-002-0531-3
pmid
12789446
Publisher site
See Article on Publisher Site

Abstract

Agrobacterium tumefaciens strain LBA4404 carrying plasmid pTOK233 encoding the hygromycin resistance ( hph ) and β-glucuronidase ( uidA ) genes has been used to transform two agronomic grass species: tall fescue (Festuca arundinacea ) and Italian ryegrass ( Lolium multiflorum ). Embryogenic cell suspension colonies or young embryogenic calli were co-cultured with Agrobacterium in the presence of acetosyringone. Colonies were grown under hygromycin selection with cefotaxime and surviving colonies plated on embryogenesis media. Eight Lolium (six independent lines) and two Festuca plants (independent lines) were regenerated and established in soil. All plants were hygromycin-resistant, but histochemical determination of GUS activity showed that only one Festuca plant and one Lolium plant expressed GUS. Three GUS-negative transgenic L. multiflorum and the two F. arundinacea plants were vernalised and allowed to flower. All three Lolium plants were male- and female-fertile, but the Festuca plants failed to produce seed. Progeny analysis of L. multiflorum showed a 24–68% inheritance of the hph and uidA genes in the three lines with no significant difference between paternal and maternal gene transmission. However, significant differences were noted between the paternal and maternal expression of hygromycin resistance.

Journal

Plant Cell Reports – Springer Journals

Published: Jan 1, 2003

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