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B. Swerdlow, B. Setlow, P. Setlow (1981)
Levels of H+ and other monovalent cations in dormant and germinating spores of Bacillus megateriumJournal of Bacteriology, 148
M. Suutari, S. Laakso (1992)
Unsaturated and branched chain-fatty acids in temperature adaptation of Bacillus subtilis and Bacillus megaterium.Biochimica et biophysica acta, 1126 2
S. Black, P. Gerhardt (1962)
Permeability of bacterial spores. III. Permeation relative to germination.Journal of bacteriology, 83
D. Cortezzo, B. Setlow, P. Setlow (2004)
Analysis of the action of compounds that inhibit the germination of spores of Bacillus speciesJournal of Applied Microbiology, 96
Aguilar P.S. (2001)
1379EMBO Journal, 45
L. Samuels, T. Uchikawa, M. Zain-ul-Abedin, R. Huseby (1969)
I – Biochemical Studies
A. Cowan, Elizabeth Olivastro, D. Koppel, C. Loshon, B. Setlow, P. Setlow (2004)
Lipids in the inner membrane of dormant spores of Bacillus species are largely immobile.Proceedings of the National Academy of Sciences of the United States of America, 101 20
B. Setlow, P. Setlow (2021)
Hydrogen peroxideReactions Weekly, 1365
Young Young, Setlow Setlow (2004b)
Mechanisms of Bacillus subtilis spore resistance to and killing by ozoneJournal of Applied Microbiology, 96
G. Rutherford, J. Reidmiller, Robert Marquis (2000)
Method to sensitize bacterial spores to subsequent killing by dry heat or ultraviolet irradiation.Journal of microbiological methods, 42 3
B. González-Flecha, B. Demple (1997)
Homeostatic regulation of intracellular hydrogen peroxide concentration in aerobically growing Escherichia coliJournal of Bacteriology, 179
J. Spizizen (1958)
TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.Proceedings of the National Academy of Sciences of the United States of America, 44 10
J. Imlay (2002)
How oxygen damages microbes: oxygen tolerance and obligate anaerobiosis.Advances in microbial physiology, 46
Yajun Song, R. Yang, Zhaobiao Guo, Minli Zhang, Xiaohui Wang, F. Zhou (2000)
Distinctness of spore and vegetative cellular fatty acid profiles of some aerobic endospore-forming bacilli.Journal of microbiological methods, 39 3
F. Tovar-Rojo, R. Cabrera-Martinez, B. Setlow, P. Setlow (2003)
Studies on the mechanism of the osmoresistance of spores of Bacillus subtilisJournal of Applied Microbiology, 95
A. Hurst (1977)
Bacterial injury: a review.Canadian journal of microbiology, 23 8
(1979)
1979b) The combined effect
A. Russell (2003)
Similarities and differences in the responses of microorganisms to biocides.The Journal of antimicrobial chemotherapy, 52 5
Michael Weber, W. Klein, L. Müller, Ulf Niess, M. Marahiel (2001)
Role of the Bacillus subtilis fatty acid desaturase in membrane adaptation during cold shockMolecular Microbiology, 39
B. Setlow, P. Setlow (1996)
Role of DNA repair in Bacillus subtilis spore resistanceJournal of Bacteriology, 178
X. Vilanova, A. Manero, M. Cerdà-Cuéllar, A. Blanch (2004)
The composition and persistence of faecal coliforms and enterococcal populations in sewage treatment plantsJournal of Applied Microbiology, 96
P. Foegeding, F. Busta (1981)
Bacterial Spore Injury - An Update 1.Journal of food protection, 44 10
L. Rode, J. Foster (1961)
GERMINATION OF BACTERIAL SPORES WITH ALKYL PRIMARY AMINESJournal of Bacteriology, 81
C. Scandella, A. Kornberg (1969)
Biochemical Studies of Bacterial Sporulation and Germination XV. Fatty Acids in Growth, Sporulation, and Germination of Bacillus megateriumJournal of Bacteriology, 98
I. Bagyan, M. Noback, S. Bron, M. Paidhungat, P. Setlow (1998)
Characterization of yhcN, a new forespore-specific gene of Bacillus subtilis.Gene, 212 2
P. Aguilar, P. López, D. Mendoza (1999)
Transcriptional Control of the Low-Temperature-Inducible des Gene, Encoding the Δ5 Desaturase of Bacillus subtilisJournal of Bacteriology, 181
(1972)
Molecular sieving by dormant spore structures
J. Gutteridge, B. Halliwell (1990)
The measurement and mechanism of lipid peroxidation in biological systems.Trends in biochemical sciences, 15 4
M. Paidhungat, P. Setlow (2002)
Germination and Outgrowth
W. Klein, Michael Weber, M. Marahiel (1999)
Cold Shock Response of Bacillus subtilis: Isoleucine-Dependent Switch in the Fatty Acid Branching Pattern for Membrane Adaptation to Low TemperaturesJournal of Bacteriology, 181
H. Fairhead, B. Setlow, P. Setlow (1993)
Prevention of DNA damage in spores and in vitro by small, acid-soluble proteins from Bacillus speciesJournal of Bacteriology, 175
A. Cazemier, S.F.M. Wagenaars, P. Steeg (2001)
Effect of sporulation and recovery medium on the heat resistance and amount of injury of spores from spoilage bacilliJournal of Applied Microbiology, 90
S. Black, P. Gerhardt (1961)
PERMEABILITY OF BACTERIAL SPORES IJournal of Bacteriology, 82
R. Simpson (1983)
The destruction of bacterial sporesJournal of Hospital Infection, 4
Young Young, Setlow Setlow (2004a)
Mechanisms of killing of spores of Bacillus subtilis by Decon and Oxone TM , two general decontaminants for biological agentsJournal of Applied Microbiology, 96
S. Young, P. Setlow (2004)
Mechanisms of killing of Bacillus subtilis spores by Decon and OxoneTM, two general decontaminants for biological agentsJournal of Applied Microbiology, 96
T. Overmire (1963)
HOMEOSTATIC REGULATION.BSCS pamphlets, 9
C. Bayliss, W. Waites (1979)
The synergistic killing of spores of Bacillus subtilis by hydrogen peroxide and ultra-violet light irradiationFems Microbiology Letters, 5
M. Cho, Hyenmi Chung, Jeyong Yoon (2003)
Quantitative evaluation of the synergistic sequential inactivation of Bacillus subtilis spores with ozone followed by chlorine.Environmental science & technology, 37 10
P. Aguilar, J. Cronan, D. Mendoza (1998)
A Bacillus subtilis Gene Induced by Cold Shock Encodes a Membrane Phospholipid DesaturaseJournal of Bacteriology, 180
B. Setlow, C. Setlow, P. Setlow (1997)
Killing bacterial spores by organic hydroperoxidesJournal of Industrial Microbiology and Biotechnology, 18
D. Gombas, R. Gómez (1978)
Sensitization of Clostridium perfringens spores to heat by gamma radiationApplied and Environmental Microbiology, 36
E. Melly, Annie Cowan, P. Setlow (2002)
Studies on the mechanism of killing of Bacillus subtilis spores by hydrogen peroxideJournal of Applied Microbiology, 93
S. Altabe, P. Aguilar, G. Caballero, D. Mendoza (2003)
The Bacillus subtilis Acyl Lipid Desaturase Is a Δ5 DesaturaseJournal of Bacteriology, 185
S. Young, P. Setlow (2003)
Mechanisms of killing of Bacillus subtilis spores by hypochlorite and chlorine dioxideJournal of Applied Microbiology, 95
N. Magill, Annie Cowan, M. Leyva-Vázquez, Martin Brown, D. Koppel, P. Setlow (1996)
Analysis of the relationship between the decrease in pH and accumulation of 3-phosphoglyceric acid in developing forespores of Bacillus speciesJournal of Bacteriology, 178
M. Nikolopoulou, J. Vary (1987)
Lipid composition ofbacillus megaterium spores and spore membranesLipids, 22
W. Nicholson, N. Munakata, G. Horneck, H. Melosh, P. Setlow (2000)
Resistance of Bacillus Endospores to Extreme Terrestrial and Extraterrestrial EnvironmentsMicrobiology and Molecular Biology Reviews, 64
C. Loshon, E. Melly, B. Setlow, P. Setlow (2001)
Analysis of the killing of spores of Bacillus subtilis by a new disinfectant, Sterilox®Journal of Applied Microbiology, 91
measurem ent and
S. Young, P. Setlow (2004)
Mechanisms of Bacillus subtilis spore resistance to and killing by aqueous ozoneJournal of Applied Microbiology, 96
B. Setlow, C. Loshon, P. Genest, A. Cowan, C. Setlow, P. Setlow (2002)
Mechanisms of killing spores of Bacillus subtilis by acid, alkali and ethanolJournal of Applied Microbiology, 92
C. Bayliss, W. Waites (1979)
The combined effect of hydrogen peroxide and ultraviolet irradiation on bacterial spores.The Journal of applied bacteriology, 47 2
(1979)
1979a) The synergistic killing
B. Setlow, A. Cowan, P. Setlow (2003)
Germination of spores of Bacillus subtilis with dodecylamineJournal of Applied Microbiology, 95
Jin-Gab Kim, A. Yousef, M. Khadre (2003)
Ozone and its current and future application in the food industry.Advances in food and nutrition research, 45
S. Hon, Y. Pyun (2001)
Membrane damage and enzyme inactivation of Lactobacillus plantarum by high pressure CO2 treatment.International journal of food microbiology, 63 1-2
E. Melly, P. Genest, Meghan Gilmore, Shawn Little, D. Popham, A. Driks, P. Setlow (2002)
Analysis of the properties of spores of Bacillus subtilis prepared at different temperaturesJournal of Applied Microbiology, 92
M. Paidhungat, B. Setlow, A. Driks, P. Setlow (2000)
Characterization of Spores of Bacillus subtilis Which Lack Dipicolinic AcidJournal of Bacteriology, 182
(1999)
Antiseptics and resistance
McDonnell McDonnell, Russell Russell (1999)
Antiseptics and resistance: activity, action and resistanceClinical Microbiology Reviews, 12
Setlow Setlow, Setlow Setlow (1993)
Binding of small, acid‐soluble spore proteins to DNA plays a significant role in the resistance of Bacillus subtilis spores to hydrogen peroxideApplied and Environmental Microbiology, 59
J. Reidmiller, J. Baldeck, G. Rutherford, R. Marquis (2003)
Characterization of UV-peroxide killing of bacterial spores.Journal of food protection, 66 7
Dye Dye, Mead Mead (1972)
The effect of chlorine on the viability of clostridial sporesJournal of Food Technology, 7
S. Ruzal, Alejandro Alice, C. Sánchez-Rivas (1994)
Osmoresistance of spores from Bacillus subtilis and the effect of ssp mutations.Microbiology, 140 ( Pt 8)
L. Wyatt, W. Waites (1975)
The effect of chlorine on spores of Clostridium bifermentans, Bacillus subtilis and Bacillus cereus.Journal of general microbiology, 89 2
B. Setlow, P. Setlow (1980)
Measurements of the pH within dormant and germinated bacterial spores.Proceedings of the National Academy of Sciences of the United States of America, 77 5
W. Crocker (1918)
Germination of SporesBotanical Gazette, 65
P. Genest, B. Setlow, E. Melly, P. Setlow (2002)
Killing of spores of Bacillus subtilis by peroxynitrite appears to be caused by membrane damage.Microbiology, 148 Pt 1
A. Moir, B. Corfe, J. Behravan (2002)
Spore germinationCellular and Molecular Life Sciences CMLS, 59
(1989)
Spore thermoresistance mechanisms
Ruth Tennen, B. Setlow, K. Davis, C. Loshon, P. Setlow (2000)
Mechanisms of killing of spores of Bacillus subtilis by iodine, glutaraldehyde and nitrous acidJournal of Applied Microbiology, 89
M. Siddiqui, Ghulam Sarwar (1999)
Antiseptics and Disinfectants: Activity, Action, and ResistanceClinical Microbiology Reviews, 12
P. Chilton, N. Isaacs, P. Manias, B. Mackey (2001)
Biosynthetic requirements for the repair of membrane damage in pressure-treated Escherichia coli.International journal of food microbiology, 71 1
P. Aguilar, A. Hernández-Arriaga, L. Cybulski, Agustin Erazo, D. Mendoza (2001)
Molecular basis of thermosensing: a two‐component signal transduction thermometer in Bacillus subtilisThe EMBO Journal, 20
C. López, H. Heras, S. Ruzal, C. Sánchez-Rivas, E. Rivas (1998)
Variations of the Envelope Composition of Bacillus subtilis During Growth in Hyperosmotic MediumCurrent Microbiology, 36
Kim Kim, Yousef Yousef, Khadre Khadre (2002)
Ozone and its current and future application in the food industryAdvances in Food Science and Nutrition, 45
Aims: To determine if treatment of Bacillus subtilis spores with a variety of oxidizing agents causes damage to the spore's inner membrane. Methods and Results: Spores of B. subtilis were killed 80–99% with wet heat or a variety of oxidizing agents, including betadine, chlorine dioxide, cumene hydroperoxide, hydrogen peroxide, OxoneTM, ozone, sodium hypochlorite and t‐butylhydroperoxide, and the agents neutralized and/or removed. Survivors of spores pretreated with oxidizing agents exhibited increased sensitivity to killing by a normally minimal lethal heat treatment, while spores pretreated with wet heat did not. In addition, spores treated with wet heat or the oxidizing agents, except sodium hypochlorite, were more sensitive to high NaCl in plating media than were untreated spores. The core region of spores treated with at least two oxidizing agents was also penetrated much more readily by methylamine than was the core of untreated spores, and spores treated with oxidizing agents but not wet heat germinated faster with dodecylamine than did untreated spores. Spores of strains with very different levels of unsaturated fatty acids in their inner membrane exhibited essentially identical resistance to oxidizing agents. Conclusions: Treatment of spores with oxidizing agents has been suggested to cause damage to the spore's inner membrane, a membrane whose integrity is essential for spore viability. The sensitization of spores to killing by heat and to high salt after pretreatment with oxidizing agents is consistent with and supports this suggestion. Presumably mild pretreatment with oxidizing agents causes some damage to the spore's inner membrane. While this damage may not be lethal under normal conditions, the damaged inner membrane may be less able to maintain its integrity, when dormant spores are exposed to high temperature or when germinated spores are faced with osmotic stress. Triggering of spore germination by dodecylamine likely involves action by this agent on the spore's inner membrane allowing release of the spore core's depot of dipicolinic acid. Presumably dodecylamine more readily alters the permeability of a damaged inner membrane and thus more readily triggers germination of spores pretreated with oxidizing agents. Damage to the inner spore membrane by oxidizing agents is also consistent with the more rapid penetration of methylamine into the core of treated spores, as the inner membrane is likely the crucial permeability barrier to methylamine entry into the spore core. As spores of strains with very different levels of unsaturated fatty acids in their inner membrane exhibited essentially identical resistance to oxidizing agents, it is not through oxidation of unsaturated fatty acids that oxidizing agents kill and/or damage spores. Perhaps these agents work by causing oxidative damage to key proteins in the spore's inner membrane. Significance and Impact of the Study: The more rapid heat killing and germination with dodecylamine, the greater permeability of the spore core and the osmotic stress sensitivity in outgrowth of spores pretreated with oxidizing agents is consistent with such agents causing damage to the spore's inner membrane, even if this damage is not lethal under normal conditions. It may be possible to take advantage of this phenomenon to devise improved, less costly regimens for spore inactivation.
Journal of Applied Microbiology – Wiley
Published: Oct 1, 2004
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