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Chitin and Chitosan Extracted from Shrimp Waste Using Fish Proteases Aided Process: Efficiency of Chitosan in the Treatment of Unhairing Effluents

Chitin and Chitosan Extracted from Shrimp Waste Using Fish Proteases Aided Process: Efficiency of... Extraction and depolymerisation of chitin and chitosan from shrimp waste material was carried out using fish proteases aided process. A high deproteinization level (80 %) was recorded with an Enzyme/Substrate ratio of 10 U/mg. The demineralization of shrimp waste was completely achieved within 6 h at room temperature in HCl 1.25 M, and the residual content of calcium in chitin was below 0.01 %. The degree of N-acetylation, calculated from the 13C CP/MAS-NMR spectrum, was 85 %. The chitin obtained was converted to chitosan by N-deacetylation. X-ray diffraction patterns also indicated two characteristics crystalline peaks approximately at 10° and 20° (2θ). Chitosan was then evaluated in the treatment of unhairing effluents from the tanning industry. A result showed that chitosan as a coagulant has good performance in alkaline pH and at concentration of 0.5 g/L. Within these conditions, chitosan could decrease turbidity value, total suspended solids (89 % at 1.5 g/L), biological oxygen demand (33.3 % at 1.5 g/L) and chemical oxygen demand (58.7 % at 1.5 g/L). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Polymers and the Environment Springer Journals

Chitin and Chitosan Extracted from Shrimp Waste Using Fish Proteases Aided Process: Efficiency of Chitosan in the Treatment of Unhairing Effluents

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References (75)

Publisher
Springer Journals
Copyright
Copyright © 2013 by Springer Science+Business Media New York
Subject
Chemistry; Polymer Sciences
ISSN
1566-2543
eISSN
1572-8900
DOI
10.1007/s10924-013-0598-7
Publisher site
See Article on Publisher Site

Abstract

Extraction and depolymerisation of chitin and chitosan from shrimp waste material was carried out using fish proteases aided process. A high deproteinization level (80 %) was recorded with an Enzyme/Substrate ratio of 10 U/mg. The demineralization of shrimp waste was completely achieved within 6 h at room temperature in HCl 1.25 M, and the residual content of calcium in chitin was below 0.01 %. The degree of N-acetylation, calculated from the 13C CP/MAS-NMR spectrum, was 85 %. The chitin obtained was converted to chitosan by N-deacetylation. X-ray diffraction patterns also indicated two characteristics crystalline peaks approximately at 10° and 20° (2θ). Chitosan was then evaluated in the treatment of unhairing effluents from the tanning industry. A result showed that chitosan as a coagulant has good performance in alkaline pH and at concentration of 0.5 g/L. Within these conditions, chitosan could decrease turbidity value, total suspended solids (89 % at 1.5 g/L), biological oxygen demand (33.3 % at 1.5 g/L) and chemical oxygen demand (58.7 % at 1.5 g/L).

Journal

Journal of Polymers and the EnvironmentSpringer Journals

Published: Jun 11, 2013

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