Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Catalytic Molecular Beacons

Catalytic Molecular Beacons We have constructed catalytic molecular beacons from a hammerhead‐type deoxyribozyme by a modular design. The deoxyribozyme was engineered to contain a molecular beacon stem–loop module that, when closed, inhibits the deoxyribozyme module and is complementary to a target oligonucleotide. Binding of target oligonucleotides opens the beacon stem–loop and allosterically activates the deoxyribozyme module, which amplifies the recognition event through cleavage of a doubly labeled fluorescent substrate. The customized modular design of catalytic molecular beacons allows for any two single‐stranded oligonucleotide sequences to be distinguished in homogenous solution in a single step. Our constructs demonstrate that antisense conformational triggers based on molecular beacons can be used to initiate catalytic events. The selectivity of the system is sufficient for analytical applications and has potential for the construction of deoxyribozyme‐based drug delivery tools specifically activated in cells containing somatic mutations. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png ChemBioChem Wiley

Catalytic Molecular Beacons

Download PDF
5 pages

Loading next page...
 
/lp/wiley/catalytic-molecular-beacons-CLmfAlZt7o

References (24)

Publisher
Wiley
Copyright
© 2001 WILEY‐VCH Verlag GmbH, Weinheim, Fed. Rep. of Germany
ISSN
1439-4227
eISSN
1439-7633
DOI
10.1002/1439-7633(20010601)2:6<411::AID-CBIC411>3.0.CO;2-I
Publisher site
See Article on Publisher Site

Abstract

We have constructed catalytic molecular beacons from a hammerhead‐type deoxyribozyme by a modular design. The deoxyribozyme was engineered to contain a molecular beacon stem–loop module that, when closed, inhibits the deoxyribozyme module and is complementary to a target oligonucleotide. Binding of target oligonucleotides opens the beacon stem–loop and allosterically activates the deoxyribozyme module, which amplifies the recognition event through cleavage of a doubly labeled fluorescent substrate. The customized modular design of catalytic molecular beacons allows for any two single‐stranded oligonucleotide sequences to be distinguished in homogenous solution in a single step. Our constructs demonstrate that antisense conformational triggers based on molecular beacons can be used to initiate catalytic events. The selectivity of the system is sufficient for analytical applications and has potential for the construction of deoxyribozyme‐based drug delivery tools specifically activated in cells containing somatic mutations.

Journal

ChemBioChem – Wiley

Published: Jun 1, 2001

There are no references for this article.