Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Ethanol Production from Alkaline Peroxide Pretreated Enzymatically Saccharified Wheat Straw

Ethanol Production from Alkaline Peroxide Pretreated Enzymatically Saccharified Wheat Straw Wheat straw used in this study contained 44.24 ± 0.28% cellulose and 25.23 ± 0.11% hemicellulose. Alkaline H2O2 pretreatment and enzymatic saccharification were evaluated for conversion of wheat straw cellulose and hemicellulose to fermentable sugars. The maximum yield of monomeric sugars from wheat straw (8.6%, w/v) by alkaline peroxide pretreatment (2.15% H2O2, v/v; pH 11.5; 35 °C; 24 h) and enzymatic saccharification (45 °C, pH 5.0, 120 h) by three commercial enzyme preparations (cellulase, β‐glucosidase, and xylanase) using 0.16 mL of each enzyme preparation per g of straw was 672 ± 4 mg/g (96.7% yield). During the pretreatment, no measurable quantities of furfural and hydroxymethyl furfural were produced. The concentration of ethanol (per L) from alkaline peroxide pretreated enzyme saccharified wheat straw (66.0 g) hydrolyzate by recombinant Escherichia coli strain FBR5 at pH 6.5 and 37 °C in 48 h was 18.9 ± 0.9 g with a yield of 0.46 g per g of available sugars (0.29 g/g straw). The ethanol concentration (per L) was 15.1 ± 0.1 g with a yield of 0.23 g/g of straw in the case of simultaneous saccharification and fermentation by the E. coli strain at pH 6.0 and 37 °C in 48 h. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology Progress Wiley

Ethanol Production from Alkaline Peroxide Pretreated Enzymatically Saccharified Wheat Straw

Biotechnology Progress , Volume 22 (2) – Jan 1, 2006

Loading next page...
 
/lp/wiley/ethanol-production-from-alkaline-peroxide-pretreated-enzymatically-C2mOHozhpA

References (0)

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
Wiley
Copyright
Copyright © 2006 American Institute of Chemical Engineers (AIChE)
ISSN
8756-7938
eISSN
1520-6033
DOI
10.1002/btpr.50310
Publisher site
See Article on Publisher Site

Abstract

Wheat straw used in this study contained 44.24 ± 0.28% cellulose and 25.23 ± 0.11% hemicellulose. Alkaline H2O2 pretreatment and enzymatic saccharification were evaluated for conversion of wheat straw cellulose and hemicellulose to fermentable sugars. The maximum yield of monomeric sugars from wheat straw (8.6%, w/v) by alkaline peroxide pretreatment (2.15% H2O2, v/v; pH 11.5; 35 °C; 24 h) and enzymatic saccharification (45 °C, pH 5.0, 120 h) by three commercial enzyme preparations (cellulase, β‐glucosidase, and xylanase) using 0.16 mL of each enzyme preparation per g of straw was 672 ± 4 mg/g (96.7% yield). During the pretreatment, no measurable quantities of furfural and hydroxymethyl furfural were produced. The concentration of ethanol (per L) from alkaline peroxide pretreated enzyme saccharified wheat straw (66.0 g) hydrolyzate by recombinant Escherichia coli strain FBR5 at pH 6.5 and 37 °C in 48 h was 18.9 ± 0.9 g with a yield of 0.46 g per g of available sugars (0.29 g/g straw). The ethanol concentration (per L) was 15.1 ± 0.1 g with a yield of 0.23 g/g of straw in the case of simultaneous saccharification and fermentation by the E. coli strain at pH 6.0 and 37 °C in 48 h.

Journal

Biotechnology ProgressWiley

Published: Jan 1, 2006

There are no references for this article.