Access the full text.
Sign up today, get DeepDyve free for 14 days.
A. Oliver, D. Hincha, L. Crowe, J. Crowe (1998)
Interactions of arbutin with dry and hydrated bilayers.Biochimica et biophysica acta, 1370 1
T. Suzuki, H. Komatsu, K. Miyajima (1996)
Effects of glucose and its oligomers on the stability of freeze-dried liposomes.Biochimica et biophysica acta, 1278 2
H. Levine, L. Slade (1988)
Principles of cryostabilization technology from structure property relationships of carbohydrate water systems a reviewCryo letters, 9
H. Nagase, Haruhisa Ueda, M. Nakagaki (1997)
Effect of water on lamellar structure of DPPC/sugar systems.Biochimica et biophysica acta, 1328 2
R. Macdonald, R. Macdonald, B. Menco, K. Takeshita, N. Subbarao, Lanying Hu (1991)
Small-volume extrusion apparatus for preparation of large, unilamellar vesicles.Biochimica et biophysica acta, 1061 2
I. Vereyken, V. Chupin, R. Demel, S. Smeekens, B. Kruijff (2001)
Fructans insert between the headgroups of phospholipids.Biochimica et biophysica acta, 1510 1-2
M. Thomashow (1999)
PLANT COLD ACCLIMATION: Freezing Tolerance Genes and Regulatory Mechanisms.Annual review of plant physiology and plant molecular biology, 50
Marion Wal, C. D’Hulst, J. Vincken, A. Buléon, R. Visser, S. Ball (1998)
Amylose Is Synthesized in Vitro by Extension of and Cleavage from Amylopectin*The Journal of Biological Chemistry, 273
K. Miyajima, K. Tomita, M. Nakagaki (1985)
Effect of saccharides on the freezing and thawing of liposome dispersion.Chemical & pharmaceutical bulletin, 34 7
D. Struck, D. Hoekstra, R. Pagano (1981)
Use of resonance energy transfer to monitor membrane fusion.Biochemistry, 20 14
W. Hinrichs, M. Prinsen, H. Frijlink (2001)
Inulin glasses for the stabilization of therapeutic proteins.International journal of pharmaceutics, 215 1-2
E. Hellwege, M. Raap, Dominique Gritscher, L. Willmitzer, A. Heyer (1998)
Differences in chain length distribution of inulin from Cynara scolymus and Helianthus tuberosus are reflected in a transient plant expression system using the respective 1‐FFT cDNAsFEBS Letters, 427
K. Ozaki, M. Hayashi (1996)
Cryoprotective effects of cycloinulohexaose on freezing and freeze-drying of liposomes.Chemical & pharmaceutical bulletin, 44 11
R. Demel, E. Dorrepaal, M. Ebskamp, J. Smeekens, B. Kruijff (1998)
Fructans interact strongly with model membranes.Biochimica et biophysica acta, 1375 1-2
A. Oliver, L. Crowe, J. Crowe (1998)
Methods for dehydration-tolerance: Depression of the phase transition temperature in dry membranes and carbohydrate vitrificationSeed Science Research, 8
H. Mantsch, R. McElhaney (1991)
Phospholipid phase transitions in model and biological membranes as studied by infrared spectroscopy.Chemistry and physics of lipids, 57 2-3
J. Roover, K. Vandenbranden, A. Laere, W. Ende (2000)
Drought induces fructan synthesis and 1-SST (sucrose: sucrose fructosyltransferase) in roots and leaves of chicory seedlings (Cichorium intybus L.)Planta, 210
John Crowe, Samuel Leslie, L. Crowe (1994)
Is vitrification sufficient to preserve liposomes during freeze-drying?Cryobiology, 31 4
D. Livingston, C. Henson (1998)
Apoplastic Sugars, Fructans, Fructan Exohydrolase, and Invertase in Winter Oat: Responses to Second-Phase Cold HardeningPlant Physiology, 116
P. Orford, R. Parker, S. Ring (1990)
Aspects of the glass transition behaviour of mixtures of carbohydrates of low molecular weight.Carbohydrate research, 196
D. Livingston (1996)
The second phase of cold hardening: freezing tolerance and fructan isomer changes in winter cereal crownsCrop Science, 36
E. Pilon-Smits, M. Ebskamp, M. Paul, M. Jeuken, P. Weisbeek, S. Smeekens (1995)
Improved Performance of Transgenic Fructan-Accumulating Tobacco under Drought Stress, 107
N. Tsvetkova, Brian Phillips, L. Crowe, John Crowe, S. Risbud (1998)
Effect of sugars on headgroup mobility in freeze-dried dipalmitoylphosphatidylcholine bilayers: solid-state 31P NMR and FTIR studies.Biophysical journal, 75 6
L. Crowe, John Crowe, Alan Rudolph, Christopher Womersley, Lori Appel (1985)
Preservation of freeze-dried liposomes by trehalose.Archives of biochemistry and biophysics, 242 1
Z. Xin, J. Browse (2000)
Cold comfort farm: the acclimation of plants to freezing temperatures.Plant Cell and Environment, 23
E. Hellwege, Dominique Gritscher, L. Willmitzer, A. Heyer (1997)
Transgenic potato tubers accumulate high levels of 1-kestose and nystose: functional identification of a sucrose sucrose 1-fructosyltransferase of artichoke (Cynara scolymus) blossom discs.The Plant journal : for cell and molecular biology, 12 5
J. Crowe, J. Crowe, A. Oliver, A. Oliver, F. Hoekstra, F. Hoekstra, L. Crowe, L. Crowe (1997)
Stabilization of dry membranes by mixtures of hydroxyethyl starch and glucose: the role of vitrification.Cryobiology, 35 1
I. Vijn, S. Smeekens (1999)
Fructan: more than a reserve carbohydrate?Plant physiology, 120 2
J. Crowe, F. Hoekstra, Khanh-Hung Nguyen, L. Crowe (1996)
Is vitrification involved in depression of the phase transition temperature in dry phospholipids?Biochimica et biophysica acta, 1280 2
D. Hincha, A. Oliver, J. Crowe (1998)
The effects of chloroplast lipids on the stability of liposomes during freezing and drying.Biochimica et biophysica acta, 1368 1
P. Steponkus (1984)
Role of the Plasma Membrane in Freezing Injury and Cold AcclimationAnnual Review of Plant Biology, 35
D. Hincha, D. Hincha, E. Hellwege, A. Heyer, J. Crowe (2000)
Plant fructans stabilize phosphatidylcholine liposomes during freeze-drying.European journal of biochemistry, 267 2
L. Schaller-Povolny, David Smith, T. Labuza (2000)
Effect of water content and molecular weight on the moisture isotherms and glass transition properties of inulinInternational Journal of Food Properties, 3
J. Crowe, J. Carpenter, L. Crowe (1998)
The role of vitrification in anhydrobiosis.Annual review of physiology, 60
C. Pollock, A. Cairns (1991)
Fructan metabolism in grasses and cereals, 42
L. Slade, H. Levine, D. Reid (1991)
Beyond water activity: recent advances based on an alternative approach to the assessment of food quality and safety.Critical reviews in food science and nutrition, 30 2-3
The fructan family of oligo- and polysaccharides is a group of molecules that have long been implicated as protective agents in the drought and freezing tolerance of many plant species. However, it has been unclear whether fructans have properties that make them better protectants for cellular structures than other sugars. We compared the effects of fructans and glucans on membrane stability during air-drying. Although glucans of increasing chain length were progressively less able to stabilize liposomes against leakage of aqueous content after rehydration, fructans showed increased protection. On the other hand, glucans became more effective in protecting liposomes against membrane fusion with increasing chain length, whereas fructans became less effective. Fourier transform infrared spectroscopy showed a reduction of the gel to liquid-crystalline phase transition temperature ( T m ) of air-dried liposomes by approximately 25°C in the presence of sucrose and maltose. For the respective pentasaccharides, the reduction of T m of the lipids was 9°C lower for samples containing fructan than for those containing glucan, indicating increased sugar–membrane interactions for the fructan compared to the glucan. A reduced interaction of the longer-chain glucans and an increased interaction of the respective fructans with the phospholipid head groups in the dry state was also indicated by dramatic differences in the phosphate asymmetric stretch region of the infrared spectrum. Collectively, our data indicate that the fructo-oligosaccharides accumulated in many plant species under stress conditions could indeed play an important role in cellular dehydration tolerance. Key words
Glycobiology – Oxford University Press
Published: Feb 1, 2002
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.