Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Drosophila Aurora B Kinase Is Required for Histone H3 Phosphorylation and Condensin Recruitment during Chromosome Condensation and to Organize the Central Spindle during Cytokinesis

Drosophila Aurora B Kinase Is Required for Histone H3 Phosphorylation and Condensin Recruitment... Aurora/Ipl1-related kinases are a conserved family of enzymes that have multiple functions during mitotic progression. Although it has been possible to use conventional genetic analysis to dissect the function of aurora , the founding family member in Drosophila (Glover, D.M., M.H. Leibowitz, D.A. McLean, and H. Parry. 1995. Cell . 81:95–105), the lack of mutations in a second aurora-like kinase gene, aurora B , precluded this approach. We now show that depleting Aurora B kinase using double-stranded RNA interference in cultured Drosophila cells results in polyploidy. aurora B encodes a passenger protein that associates first with condensing chromatin, concentrates at centromeres, and then relocates onto the central spindle at anaphase. Cells depleted of the Aurora B kinase show only partial chromosome condensation at mitosis. This is associated with a reduction in levels of the serine 10 phosphorylated form of histone H3 and a failure to recruit the Barren condensin protein onto chromosomes. These defects are associated with abnormal segregation resulting from lagging chromatids and extensive chromatin bridging at anaphase, similar to the phenotype of barren mutants (Bhat, M.A., A.V. Philp, D.M. Glover, and H.J. Bellen. 1996. Cell . 87:1103–1114.). The majority of treated cells also fail to undertake cytokinesis and show a reduced density of microtubules in the central region of the spindle. This is accompanied by a failure to correctly localize the Pavarotti kinesin-like protein, essential for this process. We discuss these conserved functions of Aurora B kinase in chromosome transmission and cytokinesis. Aurora B kinase chromosome condensation phospho-histone H3 barren cytokinesis Footnotes 1Abbreviations used in this paper: dsRNA, double-stranded RNA; INCENP, inner centromere protein; RNAi, RNA interference. Submitted: 1 November 2000 Revision requested 22 December 2000 Accepted: 29 December 2000 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Cell Biology Rockefeller University Press

Drosophila Aurora B Kinase Is Required for Histone H3 Phosphorylation and Condensin Recruitment during Chromosome Condensation and to Organize the Central Spindle during Cytokinesis

Giet, Régis; Glover, David M.
The Journal of Cell Biology , Volume 152 (4): 669 – Feb 19, 2001
Download PDF
13 pages

Loading next page...
 
/lp/rockefeller-university-press/drosophila-aurora-b-kinase-is-required-for-histone-h3-phosphorylation-BpEP1EMk0X

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
Rockefeller University Press
Copyright
© 2001 The Rockefeller University Press
ISSN
0021-9525
eISSN
1540-8140
DOI
10.1083/jcb.152.4.669
Publisher site
See Article on Publisher Site

Abstract

Aurora/Ipl1-related kinases are a conserved family of enzymes that have multiple functions during mitotic progression. Although it has been possible to use conventional genetic analysis to dissect the function of aurora , the founding family member in Drosophila (Glover, D.M., M.H. Leibowitz, D.A. McLean, and H. Parry. 1995. Cell . 81:95–105), the lack of mutations in a second aurora-like kinase gene, aurora B , precluded this approach. We now show that depleting Aurora B kinase using double-stranded RNA interference in cultured Drosophila cells results in polyploidy. aurora B encodes a passenger protein that associates first with condensing chromatin, concentrates at centromeres, and then relocates onto the central spindle at anaphase. Cells depleted of the Aurora B kinase show only partial chromosome condensation at mitosis. This is associated with a reduction in levels of the serine 10 phosphorylated form of histone H3 and a failure to recruit the Barren condensin protein onto chromosomes. These defects are associated with abnormal segregation resulting from lagging chromatids and extensive chromatin bridging at anaphase, similar to the phenotype of barren mutants (Bhat, M.A., A.V. Philp, D.M. Glover, and H.J. Bellen. 1996. Cell . 87:1103–1114.). The majority of treated cells also fail to undertake cytokinesis and show a reduced density of microtubules in the central region of the spindle. This is accompanied by a failure to correctly localize the Pavarotti kinesin-like protein, essential for this process. We discuss these conserved functions of Aurora B kinase in chromosome transmission and cytokinesis. Aurora B kinase chromosome condensation phospho-histone H3 barren cytokinesis Footnotes 1Abbreviations used in this paper: dsRNA, double-stranded RNA; INCENP, inner centromere protein; RNAi, RNA interference. Submitted: 1 November 2000 Revision requested 22 December 2000 Accepted: 29 December 2000

Journal

The Journal of Cell Biology – Rockefeller University Press

Published: Feb 19, 2001

References

  • pavarotti encodes a kinesin-like protein required to organize the central spindle and contractile ring for cytokinesis
    Adams
  • INCENP binds the aurora-related kinase AIRK2 and is required to target it to chromosomes, the central spindle and cleavage furrow
    Adams
  • Chromatid segregation at anaphase requires the barren product, a novel chromosome-associated protein that interacts with Topoisomerase II
    Bhat
  • The conserved protein kinase Ipl1 regulates microtubule binding to kinetochores in budding yeast
    Biggins
  • Reversible histone modifications and the chromosome cell cycle
    Bradbury
  • dsRNA-mediated gene silencing in cultured Drosophila cellsa tissue culture model for the analysis of RNA interference
    Caplen
  • Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways
    Clemens
  • The A-kinase–anchoring protein AKAP95 is a multivalent protein with a key role in chromatin condensation at mitosis
    Collas
  • Core histone N-termini play an essential role in mitotic chromosome condensation
    de la Barre
  • Mitotic histone H3 phosphorylation by the NIMA kinase in Asperigillus nidulans
    De Souza
  • Regulation of yeast chromosome segregation by Ipl1 protein kinase and type 1 protein phosphatase
    Francisco
  • Aurora/Ipl1p-related kinases, a new oncogenic family of mitotic serine-threonine kinases
    Giet
  • The Xenopus laevis aurora/Ip11p-related kinase pEg2 participates in the stability of the bipolar mitotic spindle
    Giet
  • The Xenopus laevis aurora-related protein kinase pEg2 associates with and phosphorylates the kinesin-related protein XlEg5
    Giet
  • Mutations in aurora prevent centrosome separation leading to the formation of monopolar spindles
    Glover
  • An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells
    Hammond
  • Sex specific processing of the Drosophila exuperentia transcript is regulated in male germline cells by the tra-2 gene
    Hazelrigg
  • Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation
    Hendzel
  • Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila barren protein
    Hirano
  • Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes
    Hsu
  • Geneticsa touch of elegance with RNAi
    Hunter
  • Gene silencingshrinking the black box of RNAi
    Hunter
  • Incenp and an Aurora-like kinase form a complex essential for chromosome segregation and efficient completion of cytokinesis
    Kaitna
  • Sli15 associates with the ipl1 protein kinase to promote proper chromosome segregation in Saccharomyces cerevisiae
    Kim
  • ATP-dependent positive supercoiling of DNA by 13S condensina biochemical implication for chromosome condensation
    Kimura
  • Phosphorylation and activation of 13S condensin by Cdc2 in vitro
    Kimura
  • 13S condensin actively reconfigures DNA by introducing global positive writheimplications for chromosome condensation
    Kimura
  • Control of apoptosis and mitotic spindle checkpoint by survivin
    Li
  • A dominant mutant of inner centromere protein (INCENP), a chromosomal protein, disrupts prometaphase congression and cytokinesis
    Mackay
  • Mitotic kinases as regulators of cell division and its checkpoints
    Nigg
  • Cloning, mapping, and expression of ial, a novel Drosophila member of the Ipl1/aurora mitotic control kinase family
    Reich
  • The Xenopus protein kinase pEg2 associates with the centrosome in a cell cycle-dependent manner, binds to the spindle microtubules and is involved in bipolar mitotic spindle assembly
    Roghi
  • Gene targeting by homologous recombination in Drosophila
    Rong
  • Regulation of Saccharomyces cerevisiae kinetochores by the type 1 phosphatase Glc7p
    Sassoon
  • A human condensin complex containing hCAP-C-hCAP-E and CNAP1, a homolog of Xenopus XCAP-D2, colocalizes with phosphorylated histone H3 during the early stage of mitotic chromosome condensation
    Schmiesing
  • AIR-2an Aurora/Ipl1-related protein kinase associated with chromosomes and midbody microtubules is required for polar body extrusion and cytokinesis in Caenorhabditis elegans embryos
    Schumacher
  • The Aurora-related kinase AIR-2 recruits ZEN-4/CeMKLP1 to the mitotic spindle at metaphase and is required for cytokinesis
    Severson
  • The survivin-like C. elegans BIR-1 protein acts with the Aurora-like kinase AIR-2 to affect chromosomes and the spindle midzone
    Speliotes
  • A kinase-anchoring protein (AKAP)95 recruits human chromosome-associated protein (hCAP)-D2/Eg7 for chromosome condensation in mitotic extract
    Steen
  • Fission yeast condensin complexessential roles of non-SMC subunits for condensation and Cdc2 phosphorylation of Cut3/SMC4
    Sutani
  • Multinuclearity and increased ploidy caused by overexpression of the aurora- and Ipl1-like midbody-associated protein mitotic kinase in human cancer cells
    Tatsuka
  • AIM-1a mammalian midbody-associated protein required for cytokinesis
    Terada
  • The product of proliferation disrupter is concentrated at centromeres and required for mitotic chromosome condensation and cell proliferation in Drosophila
    Torok
  • Phosphorylation of histone H3 is required for proper chromosome condensation and segregation
    Wei
  • The A- and B-type cyclins of Drosophila are accumulated and destroyed in temporally distinct events that define separable phases of the G2-M transition
    Whitfield
  • Stu-7/air-2 is a C. elegans aurora homologue essential for chromosome segregation during embryonic and postembryonic development
    Woolard