Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Cleavage of cellulose by a CBM33 protein

Cleavage of cellulose by a CBM33 protein Bacterial proteins categorized as family 33 carbohydrate‐binding modules (CBM33) were recently shown to cleave crystalline chitin, using a mechanism that involves hydrolysis and oxidation. We show here that some members of the CBM33 family cleave crystalline cellulose as demonstrated by chromatographic and mass spectrometric analyses of soluble products released from Avicel or filter paper on incubation with CelS2, a CBM33‐containing protein from Streptomyces coelicolor A3(2). These enzymes act synergistically with cellulases and may thus become important tools for efficient conversion of lignocellulosic biomass. Fungal proteins classified as glycoside hydrolase family 61 that are known to act synergistically with cellulases are likely to use a similar mechanism. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Protein Science Wiley

Loading next page...
 
/lp/wiley/cleavage-of-cellulose-by-a-cbm33-protein-BK2Acs0EfG

References (29)

Publisher
Wiley
Copyright
Copyright © 2011 The Protein Society
ISSN
0961-8368
eISSN
1469-896X
DOI
10.1002/pro.689
pmid
21748815
Publisher site
See Article on Publisher Site

Abstract

Bacterial proteins categorized as family 33 carbohydrate‐binding modules (CBM33) were recently shown to cleave crystalline chitin, using a mechanism that involves hydrolysis and oxidation. We show here that some members of the CBM33 family cleave crystalline cellulose as demonstrated by chromatographic and mass spectrometric analyses of soluble products released from Avicel or filter paper on incubation with CelS2, a CBM33‐containing protein from Streptomyces coelicolor A3(2). These enzymes act synergistically with cellulases and may thus become important tools for efficient conversion of lignocellulosic biomass. Fungal proteins classified as glycoside hydrolase family 61 that are known to act synergistically with cellulases are likely to use a similar mechanism.

Journal

Protein ScienceWiley

Published: Sep 1, 2011

There are no references for this article.