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Intracellular Salmonella enterica Redirect Exocytic Transport Processes in a Salmonella Pathogenicity Island 2‐Dependent Manner

Intracellular Salmonella enterica Redirect Exocytic Transport Processes in a Salmonella... During intracellular life, Salmonella enterica proliferate within a specialized membrane compartment, the Salmonella‐containing vacuole (SCV), and interfere with the microtubule cytoskeleton and cellular transport. To characterize the interaction of intracellular Salmonella with host cell transport processes, we utilized various model systems to follow microtubule‐dependent transport. The vesicular stomatitis virus glycoprotein (VSVG) is a commonly used marker to follow protein transport from the Golgi to the plasma membrane. Using a VSVG‐GFP fusion protein, we observed that virulent intracellular Salmonella alter exocytotic transport and recruit exocytotic transport vesicles to the SCV. This virulence function was dependent on the function of the type III secretion system encoded by Salmonella Pathogenicity Island 2 (SPI2) and more specifically on a subset of SPI2 effector proteins. Furthermore, the Golgi to plasma membrane traffic of the shingolipid C5‐ceramide was redirected to the SCV by virulent Salmonella. We propose that Salmonella modulates the biogenesis of the SCV by deviating this compartment from the default endocytic pathway to an organelle that interacts with the exocytic pathway. This observation might reveal a novel element of the intracellular survival and replication strategy of Salmonella. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Traffic Wiley

Intracellular Salmonella enterica Redirect Exocytic Transport Processes in a Salmonella Pathogenicity Island 2‐Dependent Manner

Traffic , Volume 7 (6) – Jun 1, 2006

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References (42)

Publisher
Wiley
Copyright
Copyright © 2006 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1398-9219
eISSN
1600-0854
DOI
10.1111/j.1600-0854.2006.00422.x
pmid
16637890
Publisher site
See Article on Publisher Site

Abstract

During intracellular life, Salmonella enterica proliferate within a specialized membrane compartment, the Salmonella‐containing vacuole (SCV), and interfere with the microtubule cytoskeleton and cellular transport. To characterize the interaction of intracellular Salmonella with host cell transport processes, we utilized various model systems to follow microtubule‐dependent transport. The vesicular stomatitis virus glycoprotein (VSVG) is a commonly used marker to follow protein transport from the Golgi to the plasma membrane. Using a VSVG‐GFP fusion protein, we observed that virulent intracellular Salmonella alter exocytotic transport and recruit exocytotic transport vesicles to the SCV. This virulence function was dependent on the function of the type III secretion system encoded by Salmonella Pathogenicity Island 2 (SPI2) and more specifically on a subset of SPI2 effector proteins. Furthermore, the Golgi to plasma membrane traffic of the shingolipid C5‐ceramide was redirected to the SCV by virulent Salmonella. We propose that Salmonella modulates the biogenesis of the SCV by deviating this compartment from the default endocytic pathway to an organelle that interacts with the exocytic pathway. This observation might reveal a novel element of the intracellular survival and replication strategy of Salmonella.

Journal

TrafficWiley

Published: Jun 1, 2006

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