Access the full text.
Sign up today, get DeepDyve free for 14 days.
M. Hindiyeh, Qi-han Li, R. Basavappa, J. Hogle, M. Chow (1999)
Poliovirus Mutants at Histidine 195 of VP2 Do Not Cleave VP0 into VP2 and VP4Journal of Virology, 73
(1998)
Structural studies of noda and tetraviruses
Christina Voorter, W. Haard-Hoekman, Piet OetelaarS, Hans Bloemendal, Wilfried JongQ (1988)
Spontaneous peptide bond cleavage in aging alpha-crystallin through a succinimide intermediate.The Journal of biological chemistry, 263 35
A. Zlotnick, V. Reddy, R. Dasgupta, A. Schneemann, W. Ray, R. Rueckert, J. Johnson (1994)
Capsid assembly in a family of animal viruses primes an autoproteolytic maturation that depends on a single aspartic acid residue.The Journal of biological chemistry, 269 18
S. Liemann, K. Chandran, T. Baker, M. Nibert, S. Harrison (2002)
Structure of the reovirus membrane-penetration protein, Mu1, in a complex with is protector protein, Sigma3.Cell, 108 2
J. Wery, V. Reddy, M. Hosur, John Johnson (1994)
The refined three-dimensional structure of an insect virus at 2.8 A resolution.Journal of molecular biology, 235 2
P. Kraulis (1991)
A program to produce both detailed and schematic plots of protein structures
T. Jones, J. Zou, S. Cowan, M. Kjeldgaard, Serge HAGgGE (1991)
Improved methods for building protein models in electron density maps and the location of errors in these models.Acta crystallographica. Section A, Foundations of crystallography, 47 ( Pt 2)
A. Fisher, John Johnson (1993)
Ordered duplex RNA controls capsid architecture in an icosahedral animal virusNature, 361
Kraulis Kraulis (1991)
MOLSCRIPT: A program to produce both detailed and schematic plots of protein structuresJ. Appl. Crystallogr., 24
X. Dong, P. Natarajan, Mariana Tihova, John Johnson, A. Schneemann (1998)
Particle Polymorphism Caused by Deletion of a Peptide Molecular Switch in a Quasiequivalent Icosahedral VirusJournal of Virology, 72
M. Canady, Mariana Tihova, T. Hanzlik, John Johnson, M. Yeager (2000)
Large conformational changes in the maturation of a simple RNA virus, Nudaurelia capensis ω virus (NωV).Journal of Molecular Biology, 299
Deepak Agrawal, John Johnson (1995)
Assembly of the T = 4 Nudaurelia capensis omega virus capsid protein, post-translational cleavage, and specific encapsidation of its mRNA in a baculovirus expression system.Virology, 207 1
K. Patel, R. Borchardt (1990)
Chemical Pathways of Peptide Degradation. II. Kinetics of Deamidation of an Asparaginyl Residue in a Model HexapeptidePharmaceutical Research, 7
T. Hanzlik, K. Gordon (1997)
The Tetraviridae.Advances in virus research, 48
M. Umashankar, M. Murthy, H. Savithri (2003)
Mutation of Interfacial Residues Disrupts Subunit Folding and Particle Assembly of Physalis mottle tymovirus *The Journal of Biological Chemistry, 278
F. Pringle, Karyn Johnson, C. Goodman, A. Mcintosh, L. Ball (2003)
Providence virus: a new member of the Tetraviridae that infects cultured insect cells.Virology, 306 2
F. Perler, Ming-Qun Xu, H. Paulus (1997)
Protein splicing and autoproteolysis mechanisms.Current opinion in chemical biology, 1 3
G. Page, A. Mosser, J. Hogle, D. Filman, R. Rueckert, M. Chow (1988)
Three-dimensional structure of poliovirus serotype 1 neutralizing determinantsJournal of Virology, 62
M. Canady, Hiro Tsuruta, John Johnson (2001)
Analysis of rapid, large-scale protein quaternary structural changes: time-resolved X-ray solution scattering of Nudaurelia capensis omega virus (NomegaV) maturation.Journal of molecular biology, 311 4
Lucia Tillotson, A. Shatkin (1992)
Reovirus polypeptide sigma 3 and N-terminal myristoylation of polypeptide mu 1 are required for site-specific cleavage to mu 1C in transfected cellsJournal of Virology, 66
J. Borsa, B. Morash, M. Sargent, T. Copps, P. Lievaart, J. Székely (1979)
Two modes of entry of reovirus particles into L cells.The Journal of general virology, 45 1
R. Briehl (1980)
Solid-like behaviour of unsheared sickle haemoglobin gels and the effects of shearNature, 288
S. Liemann, K. Chandran, T. Baker, M. Nibert, S. Harrison (2002)
Structure of the Reovirus Membrane-Penetration Protein, μ1, in a Complex with Its Protector Protein, σ3Cell, 108
C. Helgstrand, S. Munshi, John Johnson, L. Liljas (2004)
The refined structure of Nudaurelia capensis omega virus reveals control elements for a T = 4 capsid maturation.Virology, 318 1
Derek Taylor, N. Krishna, M. Canady, A. Schneemann, John Johnson (2002)
Large-Scale, pH-Dependent, Quaternary Structure Changes in an RNA Virus Capsid Are Reversible in the Absence of Subunit AutoproteolysisJournal of Virology, 76
V. Ingram (1956)
A Specific Chemical Difference Between the Globins of Normal Human and Sickle-Cell Anæmia HæmoglobinNature, 178
U. Laemmli (1970)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 227
Anette Schneemann, Weidong Zhong, Thomas Gallagher, R. Rueckert (1992)
Maturation cleavage required for infectivity of a nodavirusJournal of Virology, 66
Sanjeev Munshi, L. Liljas, Jean Cavarelli, W. Bomu, Bonnie McKinney, Vijay Reddy, John Johnson (1996)
The 2.8 A structure of a T = 4 animal virus and its implications for membrane translocation of RNA.Journal of molecular biology, 261 1
Deepak Agrawal, John Johnson (1992)
Sequence and analysis of the capsid protein of Nudaurelia capensis omega virus, an insect virus with T = 4 icosahedral symmetry.Virology, 190 2
Sangsoo Kim, T. Smith, M. Chapman, M. Rossmann, D. Pevear, F. Dutko, P. Felock, G. Diana, M. Mckinlay (1989)
Crystal structure of human rhinovirus serotype 1A (HRV1A).Journal of molecular biology, 210 1
R. Cheng, V. Reddy, N. Olson, A. Fisher, T. Baker, John Johnson (1994)
Functional implications of quasi-equivalence in a T = 3 icosahedral animal virus established by cryo-electron microscopy and X-ray crystallography.Structure, 2 4
E. Merritt, Michael Murphy (1994)
Raster3D Version 2.0. A program for photorealistic molecular graphics.Acta crystallographica. Section D, Biological crystallography, 50 Pt 6
Protein subunits of several RNA viruses are known to undergo post‐assembly, autocatalytic cleavage that is required for infectivity. Nudaurelia capensis ω virus (Nω V) is one of the simplest viruses to undergo an autocatalytic cleavage, making it an excellent model to understand both assembly and the mechanism of autoproteolysis. Heterologous expression of the coat protein gene of Nω V in a baculovirus system results in the spontaneous assembly of virus‐like particles (VLPs) that remain uncleaved when purified at neutral pH. After acidification to pH 5.0, the VLPs autocatalytically cleave at residue 570, providing an in vitro control of the cleavage. The crystal structure of Nω V displays three residues near the scissile bond that were candidates for participation in the reaction. These were changed by site‐directed mutagenesis to conservative and nonconservative residues and the products analyzed. Even conservative changes at the three residues dramatically reduced cleavage when the subunits assembled properly. Unexpectedly, we discovered that these residues are not only critical to the kinetics of Nω V autoproteolysis, but are also necessary for proper folding of subunits and, ultimately, assembly of Nω V VLPs.
Protein Science – Wiley
Published: Feb 1, 2005
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.