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High temperature triggers the metabolism of S ‐nitrosothiols in sunflower mediating a process of nitrosative stress which provokes the inhibition of ferredoxin–NADP reductase by tyrosine nitration

High temperature triggers the metabolism of S ‐nitrosothiols in sunflower mediating a process of... ABSTRACT High temperature (HT) is considered a major abiotic stress that negatively affects both vegetative and reproductive growth. Whereas the metabolism of reactive oxygen species (ROS) is well established under HT, less is known about the metabolism of reactive nitrogen species (RNS). In sunflower (Helianthus annuus L.) seedlings exposed to HT, NO content as well as S‐nitrosoglutathione reductase (GSNOR) activity and expression were down‐regulated with the simultaneous accumulation of total S‐nitrosothiols (SNOs) including S‐nitrosoglutathione (GSNO). However, the content of tyrosine nitration (NO2‐Tyr) studied by high‐performance liquid chromatography with tandem mass spectrometry (LC–MS/MS) and by confocal laser scanning microscope was induced. Nitroproteome analysis under HT showed that this stress induced the protein expression of 13 tyrosine‐nitrated proteins. Among the induced proteins, ferredoxin–NADP reductase (FNR) was selected to evaluate the effect of nitration on its activity after heat stress and in vitro conditions using 3‐morpholinosydnonimine (SIN‐1) (peroxynitrite donor) as the nitrating agent, the FNR activity being inhibited. Taken together, these results suggest that HT augments SNOs, which appear to mediate protein tyrosine nitration, inhibiting FNR, which is involved in the photosynthesis process. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell & Environment Wiley

High temperature triggers the metabolism of S ‐nitrosothiols in sunflower mediating a process of nitrosative stress which provokes the inhibition of ferredoxin–NADP reductase by tyrosine nitration

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References (89)

Publisher
Wiley
Copyright
© 2011 Blackwell Publishing Ltd
ISSN
0140-7791
eISSN
1365-3040
DOI
10.1111/j.1365-3040.2011.02376.x
pmid
21676000
Publisher site
See Article on Publisher Site

Abstract

ABSTRACT High temperature (HT) is considered a major abiotic stress that negatively affects both vegetative and reproductive growth. Whereas the metabolism of reactive oxygen species (ROS) is well established under HT, less is known about the metabolism of reactive nitrogen species (RNS). In sunflower (Helianthus annuus L.) seedlings exposed to HT, NO content as well as S‐nitrosoglutathione reductase (GSNOR) activity and expression were down‐regulated with the simultaneous accumulation of total S‐nitrosothiols (SNOs) including S‐nitrosoglutathione (GSNO). However, the content of tyrosine nitration (NO2‐Tyr) studied by high‐performance liquid chromatography with tandem mass spectrometry (LC–MS/MS) and by confocal laser scanning microscope was induced. Nitroproteome analysis under HT showed that this stress induced the protein expression of 13 tyrosine‐nitrated proteins. Among the induced proteins, ferredoxin–NADP reductase (FNR) was selected to evaluate the effect of nitration on its activity after heat stress and in vitro conditions using 3‐morpholinosydnonimine (SIN‐1) (peroxynitrite donor) as the nitrating agent, the FNR activity being inhibited. Taken together, these results suggest that HT augments SNOs, which appear to mediate protein tyrosine nitration, inhibiting FNR, which is involved in the photosynthesis process.

Journal

Plant Cell & EnvironmentWiley

Published: Nov 1, 2011

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