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COMPARTMENTATION OF CITRIC ACID CYCLE METABOLISM IN BRAIN: LABELLING OF GLUTAMATE, GLUTAMINE, ASPARTATE AND GABA BY SEVERAL RADIOACTIVE TRACER METABOLITES

COMPARTMENTATION OF CITRIC ACID CYCLE METABOLISM IN BRAIN: LABELLING OF GLUTAMATE, GLUTAMINE,... —(1) Compartmentation of the metabolism of amino acids in brain has been studied in slices of cerebral cortex incubated with sodium (1‐14C)acetate, sodium (1‐14C)‐bicarbonate, (1‐14C)GABA or l‐(1‐14C)glutamate and in samples of brain after injection in vivo of (1‐14C)‐ or (3H)acetate. (2) The method of treatment of the slices (a) maintained in ice‐cold medium prior to incubation; (b) preincubation at 37°C and transfer to fresh medium affected the metabolism of the added, labelled substrate, particularly its labelling of glutamine. (3) The specific activity of glutamine labelled from the above metabolites was greater than that of glutamic acid in experiments of 10–30 minutes duration, whether or not subjected to pretreatment in the cold. (4) Incubation in medium containing 27 mm‐K+ was associated with a decrease in the relative specific activity (RSA) of glutamine, except for the increase when l‐(1‐14C)glutamate was the precursor. (5) The data have been discussed in terms of metabolic compartmentation and their consistency with the concept of the presence in brain of more than one citric acid cycle, one containing the relatively smaller pools of intermediates and associated with synthetic processes; the other containing the relatively larger pools of intermediates and functioning as a homeostatic buffer for energy metabolism. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

COMPARTMENTATION OF CITRIC ACID CYCLE METABOLISM IN BRAIN: LABELLING OF GLUTAMATE, GLUTAMINE, ASPARTATE AND GABA BY SEVERAL RADIOACTIVE TRACER METABOLITES

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References (15)

Publisher
Wiley
Copyright
Copyright © 1970 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1970.tb02254.x
Publisher site
See Article on Publisher Site

Abstract

—(1) Compartmentation of the metabolism of amino acids in brain has been studied in slices of cerebral cortex incubated with sodium (1‐14C)acetate, sodium (1‐14C)‐bicarbonate, (1‐14C)GABA or l‐(1‐14C)glutamate and in samples of brain after injection in vivo of (1‐14C)‐ or (3H)acetate. (2) The method of treatment of the slices (a) maintained in ice‐cold medium prior to incubation; (b) preincubation at 37°C and transfer to fresh medium affected the metabolism of the added, labelled substrate, particularly its labelling of glutamine. (3) The specific activity of glutamine labelled from the above metabolites was greater than that of glutamic acid in experiments of 10–30 minutes duration, whether or not subjected to pretreatment in the cold. (4) Incubation in medium containing 27 mm‐K+ was associated with a decrease in the relative specific activity (RSA) of glutamine, except for the increase when l‐(1‐14C)glutamate was the precursor. (5) The data have been discussed in terms of metabolic compartmentation and their consistency with the concept of the presence in brain of more than one citric acid cycle, one containing the relatively smaller pools of intermediates and associated with synthetic processes; the other containing the relatively larger pools of intermediates and functioning as a homeostatic buffer for energy metabolism.

Journal

Journal of Neurochemistry – Wiley

Published: Jul 1, 1970

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