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Contribution of ornithine aminotransferase to proline accumulation in NaCl‐treated radish cotyledons

Contribution of ornithine aminotransferase to proline accumulation in NaCl‐treated radish cotyledons ABSTRACT Light‐grown cotyledons of radish (Raphanus sativus L. cv National) subjected to increasing NaCI concentrations displayed a dose‐dependent accumulation of proline at the beginning of their differentiation. Neither erythromycin nor DCMU significantly affected the salt‐induced proline accumulation, which suggests that plastids may not be involved in proline synthesis. Gabaculine has been shown previously to be a powerful and irreversible inhibitor of ornithine aminotransferase (Hervieu et al. (1993) Phyto‐chemistry 34, 231–234). This inhibitor applied in vivo at a very low concentration (1 mmol m−3) reduced considerably the salt‐induced proline accumulation of radish cotyledons. These results indicate that the ornithine pathway contributes, via an increase of ornithine aminotransferase activity, to proline synthesis, as well as the glutamate pathway, in salt‐treated cotyledons. The use of transcription and translation inhibitors revealed that the salt‐induced increase in ornithine aminotransferase activity may proceed via an activation of the translation of preformed mRNAs. The pre‐existing ornithine aminotransferase mRNAs in radish seeds may be an important adaptation to NaCI, leading to proline accumulation at the beginning of seedling growth. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell & Environment Wiley

Contribution of ornithine aminotransferase to proline accumulation in NaCl‐treated radish cotyledons

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References (36)

Publisher
Wiley
Copyright
Copyright © 1995 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0140-7791
eISSN
1365-3040
DOI
10.1111/j.1365-3040.1995.tb00354.x
Publisher site
See Article on Publisher Site

Abstract

ABSTRACT Light‐grown cotyledons of radish (Raphanus sativus L. cv National) subjected to increasing NaCI concentrations displayed a dose‐dependent accumulation of proline at the beginning of their differentiation. Neither erythromycin nor DCMU significantly affected the salt‐induced proline accumulation, which suggests that plastids may not be involved in proline synthesis. Gabaculine has been shown previously to be a powerful and irreversible inhibitor of ornithine aminotransferase (Hervieu et al. (1993) Phyto‐chemistry 34, 231–234). This inhibitor applied in vivo at a very low concentration (1 mmol m−3) reduced considerably the salt‐induced proline accumulation of radish cotyledons. These results indicate that the ornithine pathway contributes, via an increase of ornithine aminotransferase activity, to proline synthesis, as well as the glutamate pathway, in salt‐treated cotyledons. The use of transcription and translation inhibitors revealed that the salt‐induced increase in ornithine aminotransferase activity may proceed via an activation of the translation of preformed mRNAs. The pre‐existing ornithine aminotransferase mRNAs in radish seeds may be an important adaptation to NaCI, leading to proline accumulation at the beginning of seedling growth.

Journal

Plant Cell & Environment – Wiley

Published: Feb 1, 1995

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