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Reduction of human immunodeficiency virus‐ infected cells from donor blood by leukocyte filtration

Reduction of human immunodeficiency virus‐ infected cells from donor blood by leukocyte filtration Several filters for leukocyte removal were evaluated in terms of their ability to reduce the cell‐associated human immunodeficiency virus (HIV) load in units of blood either inoculated in vitro with lymphocytes from a chronically infected cell line or collected directly from seropositive donors. Filtration of the experimentally inoculated units of blood resulted in a 5.9 log10 mean reduction (95% confidence interval:7.4–4.5) of tissue culture infectious units (TCIU) as assayed by end‐point titration using the cocculture assay. Filtration of the units of blood from anti‐HIV positive donors lowered the infectivity by over 2 logs, as detected by the coculture and polymerase chain reaction (PCR) techniques. However, residual cell‐associated virus was detected in the majority of experiments. Clinical studies are warranted to determine if leukocyte filtration of blood will reduce the risk of transfusion transmitted viral infections. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Transfusion Wiley

Reduction of human immunodeficiency virus‐ infected cells from donor blood by leukocyte filtration

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References (20)

Publisher
Wiley
Copyright
1989 AABB
ISSN
0041-1132
eISSN
1537-2995
DOI
10.1046/j.1537-2995.1989.29589284150.x
Publisher site
See Article on Publisher Site

Abstract

Several filters for leukocyte removal were evaluated in terms of their ability to reduce the cell‐associated human immunodeficiency virus (HIV) load in units of blood either inoculated in vitro with lymphocytes from a chronically infected cell line or collected directly from seropositive donors. Filtration of the experimentally inoculated units of blood resulted in a 5.9 log10 mean reduction (95% confidence interval:7.4–4.5) of tissue culture infectious units (TCIU) as assayed by end‐point titration using the cocculture assay. Filtration of the units of blood from anti‐HIV positive donors lowered the infectivity by over 2 logs, as detected by the coculture and polymerase chain reaction (PCR) techniques. However, residual cell‐associated virus was detected in the majority of experiments. Clinical studies are warranted to determine if leukocyte filtration of blood will reduce the risk of transfusion transmitted viral infections.

Journal

TransfusionWiley

Published: Jun 1, 1989

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