Access the full text.
Sign up today, get DeepDyve free for 14 days.
S. Geresh, S. Arad (1991)
The extracellular polysaccharides of the red microalgae : chemistry and rheologyBioresource Technology, 38
H. Yeoh, V. Truong (1996)
Protein Contents, Amino Acid Compositions and Nitrogen‐to‐Protein Conversion Factors for Cassava RootsJournal of the Science of Food and Agriculture, 70
O. Lowry, N. Rosebrough, A. Farr, R. Randall (1951)
Protein measurement with the Folin phenol reagent.The Journal of biological chemistry, 193 1
C. López, María García, F. Fernández, C. Bustos, Y. Chisti, J. Sevilla (2010)
Protein measurements of microalgal and cyanobacterial biomass.Bioresource technology, 101 19
Mónica Fradique, A. Batista, M. Nunes, L. Gouveia, N. Bandarra, A. Raymundo (2010)
Incorporation of Chlorella vulgaris and Spirulina maxima biomass in pasta products. Part 1: Preparation and evaluation.Journal of the science of food and agriculture, 90 10
D. Crossman, K. Clements, G. Cooper (2000)
Determination of protein for studies of marine herbivory: a comparison of methodsJournal of Experimental Marine Biology and Ecology, 244
S. Lourenço, E. Barbarino, P. Lavin, U. Marquez, E. Aidar (2004)
Distribution of intracellular nitrogen in marine microalgae: Calculation of new nitrogen-to-protein conversion factorsEuropean Journal of Phycology, 39
G. Peterson (1979)
Review of the Folin phenol protein quantitation method of Lowry, Rosebrough, Farr and Randall.Analytical biochemistry, 100 2
M. Payne, R. Rippingale (2000)
Evaluation of diets for culture of the calanoid copepod Gladioferens imparipesAquaculture, 187
S. Moore, W. Stein (1948)
Photometric ninhydrin method for use in the chromatography of amino acids.The Journal of biological chemistry, 176 1
Anton Montsant, A. Zarka, S. Boussiba (2001)
Presence of a Nonhydrolyzable Biopolymer in the Cell Wall of Vegetative Cells and Astaxanthin-Rich Cysts of Haematococcus pluvialis (Chlorophyceae)Marine Biotechnology, 3
M. Mendes-Pinto, M. Raposo, J. Bowen, A. Young, R. Morais (2001)
Evaluation of different cell disruption processes on encysted cells of Haematococcus pluvialis: effects on astaxanthin recovery and implications for bio-availabilityJournal of Applied Phycology, 13
RE Lee (2008)
Phycology
C. Aflalo, Yuval Meshulam, A. Zarka, S. Boussiba (2007)
On the relative efficiency of two- vs. one-stage production of astaxanthin by the green alga Haematococcus pluvialis.Biotechnology and bioengineering, 98 1
C. Hagen, S. Siegmund, W. Braune (2002)
Ultrastructural and chemical changes in the cell wall of Haematococcus pluvialis (Volvocales, Chlorophyta) during aplanospore formationEuropean Journal of Phycology, 37
S. Fujihara, A. Kasuga, Y. Aoyagi (2001)
Nitrogen-to-Protein Conversion Factors for Common Vegetables in JapanJournal of Food Science, 66
E Barbarino, SO Lourenço (2005)
An evaluation of methodologies for extraction and quantification of protein of marine macro-and microalgaeJ Appl Phycol, 17
E. Barbarino, S. Lourenço (2005)
An evaluation of methods for extraction and quantification of protein from marine macro- and microalgaeJournal of Applied Phycology, 17
A. Vonshak (1997)
Spirulina Platensis Arthrospira : Physiology, Cell-Biology And Biotechnology
S. Arad, Michel Adda, E. Cohen (1985)
The potential of production of sulfated polysaccharides from PorphyridiumPlant and Soil, 89
R. Nguyen, H. Harvey (1994)
A rapid micro-scale method for the extraction and analysis of protein in marine samplesMarine Chemistry, 45
J. Mossé (1990)
Nitrogen-to-protein conversion factor for ten cereals and six legumes or oilseeds. A reappraisal of its definition and determination. Variation according to species and to seed protein contentJournal of Agricultural and Food Chemistry, 38
Hsueh-Kuan Lu, C. Hsieh, J. Hsu, Yuhuan Yang, H. Chou (2006)
Preventive effects of Spirulina platensis on skeletal muscle damage under exercise-induced oxidative stressEuropean Journal of Applied Physiology, 98
Michel Adda, J. Merchuk, S. Arad (1986)
Effect of nitrate on growth and production of cell-wall polysaccharide by the unicellular red alga PorphyridiumBiomass, 10
S. Lourenço, E. Barbarino, U. Marquez, E. Aidar (1998)
DISTRIBUTION OF INTRACELLULAR NITROGEN IN MARINE MICROALGAE: BASIS FOR THE CALCULATION OF SPECIFIC NITROGEN‐TO‐PROTEIN CONVERSION FACTORSJournal of Phycology, 34
E. Becker (1994)
Microalgae: Biotechnology and Microbiology
N. Sriperm, G. Pesti, P. Tillman (2011)
Evaluation of the fixed nitrogen-to-protein (N:P) conversion factor (6.25) versus ingredient specific N:P conversion factors in feedstuffs.Journal of the science of food and agriculture, 91 7
K. Loubière, J. Pruvost, F. Aloui, J. Legrand (2011)
Investigations in an external-loop airlift photobioreactor with annular light chambers and swirling flowChemical Engineering Research & Design, 89
S. Lourenço, E. Barbarino, J. De-Paula, Luis Pereira, U. Marquez (2002)
Amino acid composition, protein content and calculation of nitrogen‐to‐protein conversion factors for 19 tropical seaweedsPhycological Research, 50
S. Arad, O. Friedman, A. Rotem (1988)
Effect of Nitrogen on Polysaccharide Production in a Porphyridium spApplied and Environmental Microbiology, 54
T. Sobczuk, Ae Garcıá, Camacho Molina, Grima Ae, Y. Chisti (2006)
Effects of agitation on the microalgae Phaeodactylum tricornutum and Porphyridium cruentumBioprocess and Biosystems Engineering, 28
G. Diniz, E. Barbarino, J. Oiano-Neto, S. Pacheco, S. Lourenço (2011)
Gross Chemical Profile and Calculation of Nitrogen-to-Protein Conversion Factors for Five Tropical SeaweedsAmerican Journal of Plant Sciences, 02
S. Geresh, A. Mamontov, J. Weinstein (2002)
Sulfation of extracellular polysaccharides of red microalgae: preparation, characterization and properties.Journal of biochemical and biophysical methods, 50 2-3
Additional evidence about the influence of the cell wall physical and chemical characteristics on protein extractability was determined by calculating the conversion factors of five different microalgae known to have different cell wall composition, and their protein extracts. The conversion factors obtained for crude rigid cell walled Chlorella vulgaris, Nannochloropsis oculata and Haematococcus pluvialis were 6.35, 6.28 and 6.25, respectively, but for their protein extracts the values were lower with 5.96, 5.86 and 5.63. On the other hand, conversion factor obtained for fragile cell walled microalgae Porphyridium cruentum and Athrospira platensis was 6.35 for the former and 6.27 for the latter, with no significant difference for their protein extract with 6.34 for the former and 6.21 for the latter. In addition, the highest hydro-soluble protein percentage recovered from total protein was for P. cruentum 80.3 % and A. platensis 69.5 % but lower for C. vulgaris with 43.3 %, N. oculata with 33.3 % and H. pluvialis with 27.5 %. The study spotted the light on the influence of the cell wall on evaluating the conversion factor and protein extractability. In addition, it showed the necessity of finding the conversion factor every time accurate protein quantification is required, and proved that there is not a universal conversion factor that can be recommended.
Journal of Applied Phycology – Springer Journals
Published: Aug 8, 2012
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.