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PROTECTION BY AET AGAINST INACTIVATION OF TRANSFORMING DNA BY NEAR‐ULTRAVIOLET LIGHT: ACTION SPECTRUM *

PROTECTION BY AET AGAINST INACTIVATION OF TRANSFORMING DNA BY NEAR‐ULTRAVIOLET LIGHT: ACTION... RESEARCH NOTE PROTECTION BY AET AGAINST INACTIVATION OF TRANSFORMING DNA BY NEAR-ULTRAVIOLET LIGHT: ACTION SPECTRUM* M. J. PEAK? and J. G . PEAK? Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois 60439, U S A . (Received 11 March 1975; accepted 14 M a y 1975) We have previously shown that histidine protects Bacillus subtilis DNA from inactivation by near-ultraviolet (near UV) radiation, but not by far-ultraviolet (far UV) radiation (Peak et al., 1973b). This observation indicated that the processes of inactivation of transforming DNA by near UV are not the same as for far UV. In this note,' we describe the protective action of another compound, 2-aminoethylisothiouronium bromide hydrobromide (AET). The fact that histidine protects against the actions of both near UV and X rays prompted us to compare the histidine protection against near UV with AET protection against near UV, since this latter compound also protects biological material against X-ray action (Doherty, 1960; Doherty and Burnett, 1955). METHODS lO.00 &-a TRYPTOPHAN+AET 0.lOb LEUCINE, CONTROL H TRYPTOPHAN, CONTROL 0.0I Strains, preparation of competent cells and transforming DNA, and irradiation techniques were as described previously (Peak et al., 1973a). AET, lot 300077, was purchased from http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Photochemistry & Photobiology Wiley

PROTECTION BY AET AGAINST INACTIVATION OF TRANSFORMING DNA BY NEAR‐ULTRAVIOLET LIGHT: ACTION SPECTRUM *

Peak, M. J.; Peak, J. G.
Photochemistry & Photobiology , Volume 22 (3‐4) – Sep 1, 1975
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References (9)

Publisher
Wiley
Copyright
Copyright © 1975 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0031-8655
eISSN
1751-1097
DOI
10.1111/j.1751-1097.1975.tb08828.x
Publisher site
See Article on Publisher Site

Abstract

RESEARCH NOTE PROTECTION BY AET AGAINST INACTIVATION OF TRANSFORMING DNA BY NEAR-ULTRAVIOLET LIGHT: ACTION SPECTRUM* M. J. PEAK? and J. G . PEAK? Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois 60439, U S A . (Received 11 March 1975; accepted 14 M a y 1975) We have previously shown that histidine protects Bacillus subtilis DNA from inactivation by near-ultraviolet (near UV) radiation, but not by far-ultraviolet (far UV) radiation (Peak et al., 1973b). This observation indicated that the processes of inactivation of transforming DNA by near UV are not the same as for far UV. In this note,' we describe the protective action of another compound, 2-aminoethylisothiouronium bromide hydrobromide (AET). The fact that histidine protects against the actions of both near UV and X rays prompted us to compare the histidine protection against near UV with AET protection against near UV, since this latter compound also protects biological material against X-ray action (Doherty, 1960; Doherty and Burnett, 1955). METHODS lO.00 &-a TRYPTOPHAN+AET 0.lOb LEUCINE, CONTROL H TRYPTOPHAN, CONTROL 0.0I Strains, preparation of competent cells and transforming DNA, and irradiation techniques were as described previously (Peak et al., 1973a). AET, lot 300077, was purchased from

Journal

Photochemistry & Photobiology – Wiley

Published: Sep 1, 1975

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