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Expression analysis of BsAPase14 acid phosphatase, a stress responsive boiling-stable protein from Triticum aestivum

Expression analysis of BsAPase14 acid phosphatase, a stress responsive boiling-stable protein... Abstract Developmental regulation and effect of abiotic stresses (osmotic, salt, heat, and drought) on induction of boiling-stable acid phosphatases (BsAPases) was studied in the growing wheat seedlings. SDS-PAGE revealed the induction of several boiling-stable polypeptides in a spatial and temporal manner in the growing seedlings. Western blot analysis, using polyclonal acid phosphatase antiserum, revealed the induction of a cross-reacting one 14 kDa boiling-stable acid phosphatase band (14 kDa, BsAPase14) in endosperm at 7 and 14 days of germination (DOG). Various abiotic stresses (heat, salt, and osmotic) also elicit accumulation of BsAPase14 in a tissue-dependent manner. Imposition of drought stress also resulted in an induction of boiling-stable protein (BsAPase-14) which disappeared after removing the stress. Based upon these observations, the possible role of BsAPase14 in water-stress tolerance is discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Crop Science and Biotechnology Springer Journals

Expression analysis of BsAPase14 acid phosphatase, a stress responsive boiling-stable protein from Triticum aestivum

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References (22)

Publisher
Springer Journals
Copyright
2012 Korean Society of Crop Science and Springer Netherlands
ISSN
1975-9479
eISSN
2005-8276
DOI
10.1007/s12892-011-0054-y
Publisher site
See Article on Publisher Site

Abstract

Abstract Developmental regulation and effect of abiotic stresses (osmotic, salt, heat, and drought) on induction of boiling-stable acid phosphatases (BsAPases) was studied in the growing wheat seedlings. SDS-PAGE revealed the induction of several boiling-stable polypeptides in a spatial and temporal manner in the growing seedlings. Western blot analysis, using polyclonal acid phosphatase antiserum, revealed the induction of a cross-reacting one 14 kDa boiling-stable acid phosphatase band (14 kDa, BsAPase14) in endosperm at 7 and 14 days of germination (DOG). Various abiotic stresses (heat, salt, and osmotic) also elicit accumulation of BsAPase14 in a tissue-dependent manner. Imposition of drought stress also resulted in an induction of boiling-stable protein (BsAPase-14) which disappeared after removing the stress. Based upon these observations, the possible role of BsAPase14 in water-stress tolerance is discussed.

Journal

Journal of Crop Science and BiotechnologySpringer Journals

Published: Mar 1, 2012

Keywords: Plant Sciences; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Biotechnology

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