Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

An Ry‐mediated resistance response in potato requires the intact active site of the NIa proteinase from potato virus Y

An Ry‐mediated resistance response in potato requires the intact active site of the NIa... Ry confers extreme resistance to all strains of potato virus Y (PVY). To identify the elicitor of the Ry‐mediated resistance against PVY in potato, we expressed each of the PVY‐encoded proteins in leaves of PVY‐resistant (Ry) and ‐susceptible (ry) plants. For most of the proteins tested, there was no evident response. However, when the NIa proteinase was expressed in leaves of Ry plants, there was a hypersensitive response (HR). Proteinase active site mutants failed to induce the Ry‐mediated response. The HR was also induced by the NIa proteinase from pepper mottle virus (PepMoV), which has the same cleavage specificity as the PVY enzyme, but not by the tobacco etch virus (TEV) or the potato virus A (PVA) proteinases that cleave different peptide motifs. Based on these results, we propose that Ry‐mediated resistance requires the intact active site of the NIa proteinase. Although the structure of the active proteinase could have elicitor activity, it is possible that this proteinase releases an elicitor by cleavage of a host‐encoded protein. Alternatively, the proteinase could inactivate a negative regulator of the Ry‐mediated resistance response. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Plant Journal Wiley

An Ry‐mediated resistance response in potato requires the intact active site of the NIa proteinase from potato virus Y

Loading next page...
 
/lp/wiley/an-ry-mediated-resistance-response-in-potato-requires-the-intact-5Kp5eV4fGu

References (53)

Publisher
Wiley
Copyright
Copyright © 2000 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0960-7412
eISSN
1365-313X
DOI
10.1046/j.1365-313x.2000.00834.x
Publisher site
See Article on Publisher Site

Abstract

Ry confers extreme resistance to all strains of potato virus Y (PVY). To identify the elicitor of the Ry‐mediated resistance against PVY in potato, we expressed each of the PVY‐encoded proteins in leaves of PVY‐resistant (Ry) and ‐susceptible (ry) plants. For most of the proteins tested, there was no evident response. However, when the NIa proteinase was expressed in leaves of Ry plants, there was a hypersensitive response (HR). Proteinase active site mutants failed to induce the Ry‐mediated response. The HR was also induced by the NIa proteinase from pepper mottle virus (PepMoV), which has the same cleavage specificity as the PVY enzyme, but not by the tobacco etch virus (TEV) or the potato virus A (PVA) proteinases that cleave different peptide motifs. Based on these results, we propose that Ry‐mediated resistance requires the intact active site of the NIa proteinase. Although the structure of the active proteinase could have elicitor activity, it is possible that this proteinase releases an elicitor by cleavage of a host‐encoded protein. Alternatively, the proteinase could inactivate a negative regulator of the Ry‐mediated resistance response.

Journal

The Plant JournalWiley

Published: Sep 1, 2000

Keywords: ; ; ; ; ;

There are no references for this article.