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Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats

Amplification of DNA markers from evolutionarily diverse genomes using single primers of... 122 89 89 7 8 M. Gupta Y.-S. Chyi J. Romero-Severson J. L. Owen Madison Laboratories Mycogen Plant Sciences 5649 E. Buckeye Road 53714 Madison WI USA Life Technologies Inc. 8717 Grovemont Circle 20884 Gaithersburg MD USA Abstract The abundance and scattered distribution of simple-sequence repeats (SSR) in eukaryotic genomes prompted us to explore the use of SSR-based oligonucleotide primers in single primer amplification reactions. In a pilot experiment, 23 primers were used across a panel of evolutionarily diverse eukaryotic genomes, including grapes, lettuce, tomato, pine, maize, salmon, chicken, Holstein cows and humans. The primers were 16–20 bases in length and represented SSRs of di-, tri-, tetra-, and pentanucleotide repeats. The results showed that tetranucleotide repeat primers were most effective in amplifying polymorphic patterns. Of 11 such primers tested, 70% produced polymorphic patterns from the DNA of one or more species. Primers representing a combination of two tetranucleotide repeats, or compound microsatellites, were equally effective. The polymorphisms contained in such fingerprints were able to identify individuals of vertebrate species as well as lines or varieties of plants. Inheritance of the polymorphic bands was studied in a maize recombinant inbred population, DE811 x B73. Thirty-two polymorphic bands, derived from two amplification patterns, were mapped as dominant markers on an existing RFLP map of the same population. The bands were distributed across nine of the ten chromosomes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png TAG Theoretical and Applied Genetics Springer Journals

Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats

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References (34)

Publisher
Springer Journals
Copyright
Copyright © 1994 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Agriculture; Biochemistry, general; Plant Biochemistry; Plant Sciences; Plant Genetics & Genomics
ISSN
0040-5752
eISSN
1432-2242
DOI
10.1007/BF00224530
pmid
24178116
Publisher site
See Article on Publisher Site

Abstract

122 89 89 7 8 M. Gupta Y.-S. Chyi J. Romero-Severson J. L. Owen Madison Laboratories Mycogen Plant Sciences 5649 E. Buckeye Road 53714 Madison WI USA Life Technologies Inc. 8717 Grovemont Circle 20884 Gaithersburg MD USA Abstract The abundance and scattered distribution of simple-sequence repeats (SSR) in eukaryotic genomes prompted us to explore the use of SSR-based oligonucleotide primers in single primer amplification reactions. In a pilot experiment, 23 primers were used across a panel of evolutionarily diverse eukaryotic genomes, including grapes, lettuce, tomato, pine, maize, salmon, chicken, Holstein cows and humans. The primers were 16–20 bases in length and represented SSRs of di-, tri-, tetra-, and pentanucleotide repeats. The results showed that tetranucleotide repeat primers were most effective in amplifying polymorphic patterns. Of 11 such primers tested, 70% produced polymorphic patterns from the DNA of one or more species. Primers representing a combination of two tetranucleotide repeats, or compound microsatellites, were equally effective. The polymorphisms contained in such fingerprints were able to identify individuals of vertebrate species as well as lines or varieties of plants. Inheritance of the polymorphic bands was studied in a maize recombinant inbred population, DE811 x B73. Thirty-two polymorphic bands, derived from two amplification patterns, were mapped as dominant markers on an existing RFLP map of the same population. The bands were distributed across nine of the ten chromosomes.

Journal

TAG Theoretical and Applied GeneticsSpringer Journals

Published: Dec 1, 1994

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