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E. Kelly, M. Keen, P. Nobbs, J. Macdermot (1990)
Segregation of discrete Gsα‐mediated responses that accompany homologous or heterologous desensitization in two related somatic hybridsBritish Journal of Pharmacology, 99
Y. Salomon, C. Londos, M. Rodbell (1974)
A highly sensitive adenylate cyclase assay.Analytical biochemistry, 58 2
R. Edwards, J. Macdermot, Amanda Wilkins (1987)
Prostacyclin analogues reduce ADP‐ribosylation of the α‐subunit of the regulatory Gs‐protein and diminish adenosine (A2) responsiveness of plateletsBritish Journal of Pharmacology, 90
E. Neer, D. Clapham (1988)
Roles of G protein subunits in transmembrane signallingNature, 333
A. Levitzki (1987)
Regulation of hormone-sensitive adenylate cyclaseTrends in Pharmacological Sciences, 8
P. Sternweis, J. Northup, M. Smigel, A. Gilman (1981)
The regulatory component of adenylate cyclase. Purification and properties.The Journal of biological chemistry, 256 22
V. Manganiello, M. Vaughan (1976)
Activation and inhibition of fat cell adenylate cyclase by fluoride.The Journal of biological chemistry, 251 20
(1989)
Lithium enhances the
K. Seamon, J. Daly (1982)
Guanosine 5'-(beta, gamma-imido)triphosphate inhibition of forskolin-activated adenylate cyclase is mediated by the putative inhibitory guanine nucleotide regulatory protein.The Journal of biological chemistry, 257 19
K. Jakobs, K. Aktories, G. Schultz (1984)
Mechanisms and components involved in adenylate cyclase inhibition by hormones.Advances in cyclic nucleotide and protein phosphorylation research, 17
J. Stadel, S. Crooke (1989)
Fluoride interaction with G-proteins.The Biochemical journal, 258 3
K. Jakobs, K. Aktories, M. Minuth, G. Schultz (1985)
Inhibition of adenylate cyclase.Advances in cyclic nucleotide and protein phosphorylation research, 19
E. Ross, A. Gilman (1980)
Biochemical properties of hormone-sensitive adenylate cyclase.Annual review of biochemistry, 49
M. Chabre (1989)
Aluminofluoride action on G-proteins of the adenylate cyclase system is not different from that on transducin.The Biochemical journal, 258 3
T. Harden (1983)
Agonist-induced desensitization of the beta-adrenergic receptor-linked adenylate cyclase.Pharmacological reviews, 35 1
A. Howlett, P. Sternweis, B. Macik, P. Arsdale, Alfred Gilman (1979)
Reconstitution of catecholamine-sensitive adenylate cyclase. Association of a regulatory component of the enzyme with membranes containing the catalytic protein and beta-adrenergic receptors.The Journal of biological chemistry, 254 7
R. Clark (1986)
Desensitization of hormonal stimuli coupled to regulation of cyclic AMP levels.Advances in cyclic nucleotide and protein phosphorylation research, 20
Seamon Kb, Daly Jw (1986)
Forskolin: its biological and chemical properties.Advances in cyclic nucleotide and protein phosphorylation research, 20
(1982)
Modulation by Isletactivating protein
P. Godfrey, S. Watson (1988)
Fluoride inhibits agonist-induced formation of inositol phosphates in rat cortex.Biochemical and biophysical research communications, 155 2
Seamon Kb, Daly Jw (1985)
High-affinity binding of forskolin to rat brain membranes.Advances in cyclic nucleotide and protein phosphorylation research, 19
T. Katada, T. Amano, M. Ui (1982)
Modulation by islet-activating protein of adenylate cyclase activity in C6 glioma cells.The Journal of biological chemistry, 257 7
HOWLETT (1979)
Reconstitution of catecholamine-sensitive adenylate cyclase.J. Biol. Chem., 254
C. Londos, Y. Salomon, M. Lin, J. Harwood, M. Schramm, J. Wolff, M. Rodbell (1974)
5'-Guanylylimidodiphosphate, a potent activator of adenylate cyclase systems in eukaryotic cells.Proceedings of the National Academy of Sciences of the United States of America, 71 8
L. Birnbaumer (1987)
Which G protein subunits are the active mediators in signal transductionTrends in Pharmacological Sciences, 8
WHITWORTH (1989)
Lithium enhances the inhibitory effects of fluoroaluminate on inositol phospholipid hydrolysis.Br. J. Pharmacol., 97
K. Rich, J. Codina, G. Floyd, R. Sekura, J. Hildebrandt, R. Iyengar (1984)
Glucagon-induced heterologous desensitization of the MDCK cell adenylyl cyclase. Increases in the apparent levels of the inhibitory regulator (Ni).The Journal of biological chemistry, 259 12
Emanuel HanskiS, P. Sternweis, J. Northup, A. Dromerick, A. Gilman (1981)
The Regulatory Component of Adenylate Cyclase
M. Ui (1984)
Islet-activating protein, pertussis toxin: a probe for functions of the inhibitory guanine nucleotide regulatory component of adenylate cyclaseTrends in Pharmacological Sciences, 5
Patrick Casey, Alfred Gilman (1988)
G protein involvement in receptor-effector coupling.The Journal of biological chemistry, 263 6
John MacDermot, Haruhiro Higashida, Steven Wilson, Hiroshi Matsuzawa, John Minna, Marshall Nirenberg (1979)
Adenylate cyclase and acetylcholine release regulated by separate serotonin receptors of somatic cell hybrids.Proceedings of the National Academy of Sciences of the United States of America, 76 3
J. Bigay, P. Deterre, C. Pfister, M. Chabre (1985)
Fluoroaluminates activate transducin‐GDP by mimicking the γ‐phosphate of GTP in its binding siteFEBS Letters, 191
M. Garrity, T. Andreasen, D. Storm, R. Robertson (1983)
Prostaglandin E-induced heterologous desensitization of hepatic adenylate cyclase. Consequences on the guanyl nucleotide regulatory complex.The Journal of biological chemistry, 258 14
K. Jakobs, K. Aktories, G. Schultz (1984)
Mechanism of pertussis toxin action on the adenylate cyclase system. Inhibition of the turn-on reaction of the inhibitory regulatory site.European journal of biochemistry, 140 1
T. Katada, G. Bokoch, J. Northup, M. Ui, A. Gilman (1984)
The inhibitory guanine nucleotide-binding regulatory component of adenylate cyclase. Properties and function of the purified protein.The Journal of biological chemistry, 259 6
A. Spiegel (1987)
Signal transduction by guanine nucleotide binding proteinsMolecular and Cellular Endocrinology, 49
T. Katada, J. Northup, G. Bokoch, M. Ui, A. Gilman (1984)
The inhibitory guanine nucleotide-binding regulatory component of adenylate cyclase. Subunit dissociation and guanine nucleotide-dependent hormonal inhibition.The Journal of biological chemistry, 259 6
A. Gilman (1987)
G proteins: transducers of receptor-generated signals.Annual review of biochemistry, 56
1 NaF (10 mm) produced a 2–3 fold increase in adenylate cyclase activity in homogenates of NG108‐15 cells incubated in the presence of 1 μm GTP. Higher concentrations of NaF suppressed adenylate cyclase activity. 2 In the presence of the adenosine receptor agonist 5′‐(N‐ethyl)‐carboxamidoadenosine (NECA; 100 μm) or the prostacyclin receptor agonist iloprost (10 nm), NaF produced a much smaller increase in adenylate cyclase activity, whereas in the presence of a saturating concentration of iloprost (1 μm), NaF only inhibited adenylate cyclase activity. 3 Similarly, Gpp(NH)p activated basal adenylate cyclase activity, and inhibited 1 μm iloprost‐activated enzyme activity. In the presence of 10 μm forskolin, NaF or Gpp(NH)p increased adenylate cyclase activity synergistically. Analysis of concentration‐effect curves indicated that NaF (2 mm) or Gpp(NH)p (100 μm) increased the potency with which forskolin activated adenylate cyclase, whilst reducing the maximum activation of adenylate cyclase by iloprost. 4 Opiate receptors mediate inhibition of adenylate cyclase, and the opiate agonist morphine (100 μm) reduced the capacity of NaF or Gpp(NH)p to inhibit iloprost‐activated adenylate cyclase. Unexpectedly, pertussis toxin treatment enhanced the ability of NaF or Gpp(NH)p to inhibit iloprost‐activated adenylate cyclase. 5 In the absence of GTP, NaF and Gpp(NH)p remained able both to activate basal adenylate cyclase and to be synergistic with forskolin in activating the enzyme. In contrast the ability of NaF and Gpp(NH)p to inhibit iloprost‐activated adenylate cyclase was substantially lost in the absence of added GTP. 6 These results suggest that NaF modulates adenylate cyclase activity in NG108‐15 cell membranes by interacting with the α subunits of both Gs and Gi regulatory proteins. The effects of NaF and Gpp(NH)p are critically dependent on the prior mode and extent of activation or inhibition of this transmembrane signalling pathway. This simple system may be of use in assessing alterations in Gs‐Gi interaction following manipulations such as hormone receptor desensitization.
British Journal of Pharmacology – Wiley
Published: Jun 1, 1990
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