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Comparative sequence analysis of A-type inclusion (ATI) and P4c proteins of orthopoxviruses that produce typical and atypical ATI phenotypes

Comparative sequence analysis of A-type inclusion (ATI) and P4c proteins of orthopoxviruses that... Some orthopoxviruses produce large proteinaceous intracellular bodies, known as A-type inclusions (ATIs) during infection of host cells. Virions associate with ATIs resulting in distinct phenotypes referred to as V+, V+/ and V−. The phenotype V+ has the virions embedded in the ATI matrix; V− has no virions embedded within or on the surface of the ATI matrix, whereas an aberrant phenotype, the V+/ has virions only on the surface of ATIs. Viruses that do not produce ATI are designated as V0. Recombinant viruses generated from a V+ cowpox virus (CPXV) and a V0 transgenic vaccinia virus (VACV) produced aberrant V+/ ATIs. ATI phenotype is dependent on the A-type inclusion protein (Atip) and the P4c protein. We sequenced the atip and p4c genes of parental and progeny recombinant viruses as well as their flanking sequences. The atip and p4c open reading frames were identical in parental V+ CPXV and hybrid V+/ progenies. Our results suggest that additional viral gene(s) are required for the formation of wild type V+ ATI. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Virus Genes Springer Journals

Comparative sequence analysis of A-type inclusion (ATI) and P4c proteins of orthopoxviruses that produce typical and atypical ATI phenotypes

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References (21)

Publisher
Springer Journals
Copyright
Copyright © Springer Science+Business Media, LLC 2009
Subject
Biomedicine; Plant Sciences ; Virology ; Medical Microbiology
ISSN
0920-8569
eISSN
1572-994X
DOI
10.1007/s11262-009-0376-8
pmid
19533319
Publisher site
See Article on Publisher Site

Abstract

Some orthopoxviruses produce large proteinaceous intracellular bodies, known as A-type inclusions (ATIs) during infection of host cells. Virions associate with ATIs resulting in distinct phenotypes referred to as V+, V+/ and V−. The phenotype V+ has the virions embedded in the ATI matrix; V− has no virions embedded within or on the surface of the ATI matrix, whereas an aberrant phenotype, the V+/ has virions only on the surface of ATIs. Viruses that do not produce ATI are designated as V0. Recombinant viruses generated from a V+ cowpox virus (CPXV) and a V0 transgenic vaccinia virus (VACV) produced aberrant V+/ ATIs. ATI phenotype is dependent on the A-type inclusion protein (Atip) and the P4c protein. We sequenced the atip and p4c genes of parental and progeny recombinant viruses as well as their flanking sequences. The atip and p4c open reading frames were identical in parental V+ CPXV and hybrid V+/ progenies. Our results suggest that additional viral gene(s) are required for the formation of wild type V+ ATI.

Journal

Virus GenesSpringer Journals

Published: Oct 1, 2009

Keywords: Cowpox virus; MVA; A-type inclusion; P4c; Sequence analysis; Recombination

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