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- Publisher
- Springer Journals
- Copyright
- Copyright © 1998 by Springer-Verlag Berlin Heidelberg
- Subject
- Legacy
- ISSN
- 0721-7714
- eISSN
- 1432-203X
- DOI
- 10.1007/s002990050387
- Publisher site
- See Article on Publisher Site
Abstract
Totipotent calli of Cymbidium ensifolium var. misericors, a locally grown orchid of high commercial value, were induced from sections of pseudobulbs, rhizomes and roots of seed-derived plantlets on 1/2-strength Murashige and Skoog medium plus 10 mg/l 2,4-dichlorophenoxyacetic acid and 0.1 mg/l thiadiazuron. The calli could be maintained by subculturing in the same medium. The calli could be induced to develop further along one of three distinct morphogeneic routes: (1) production of rhizomes, (2) production of shoot buds, or (3) development of granular embryoids. Efficient mass propagation was possible via rhizome proliferation and embryoid formation.
Journal
Plant Cell Reports – Springer Journals
Published: Feb 1, 1998