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M. Guy, L. Reinhold, M. Rahat, A. Seiden (1981)
Protonation and light synergistically convert plasmalemma sugar carrier system in mesophyll protoplasts to its fully activated form.Plant physiology, 67 6
Lemoine Lemoine, Delrot Delrot, Gallet Gallet, Larsson Larsson (1989)
The sucrose carrier of the plant plasma membrane. II. Immunological evidenceBiochimica & Biophysica Acta, 78
J. Riesmeier, B. Hirner, Woff Frommer (1993)
Potato sucrose transporter expression in minor veins indicates a role in phloem loading.The Plant cell, 5
Michael Palmgren, M. Sommarin (1989)
Lysophosphatidylcholine stimulates ATP dependent proton accumulation in isolated oat root plasma membrane vesicles.Plant physiology, 90 3
R. Lemoine, S. Delrot, O. Gallet, C. Larsson (1989)
The sucrose carrier of the plant plasma membrane. II. Immunological characterizationBiochimica et Biophysica Acta, 978
Norbert Sauer, JLirgen Stolz (1994)
SUC1 and SUC2: two sucrose transporters from Arabidopsis thaliana; expression and characterization in baker's yeast and identification of the histidine-tagged protein.The Plant journal : for cell and molecular biology, 6 1
Lemoine Lemoine, Gallet Gallet, Gaillard Gaillard, Frommer Frommer, Delrot Delrot (1992)
Plasma membrane vesicle's from source and sink leavesPlant Physiology, 100
Truernit Truernit, Sauer Sauer (1995)
The promoter of She Arabidopsis thaliana SUC2 sucrose‐H+ symporter gene directs expression of β‐glucuronidase to the phloem: evidence for phloem loading and unloading by SUC2Plant, 196
J. Riesmeier, L. Willmitzer, W. Frommer (1994)
Evidence for an essential role of the sucrose transporter in phloem loading and assimilate partitioning.The EMBO Journal, 13
D. Bush (1993)
Proton-Coupled Sugar and Amino Acid Transporters in Plants, 44
Palmgren Palmgren, Sommarin Sommarin (1989)
Lysophosphatidylcholine stimulates ATP dependent proton accumulation in isolated root PMVPlant Physiology, 90
Orlich Orlich, Komor Komor (1992)
Phloem Loading in sucrose cotyledons: sucrose pathways via the mesophyll and the apoplasmPlanta, 187
R. Lemoine, O. Gallet, C. Gaillard, W. Frommer, S. Delrot (1992)
Plasma membrane vesicles from source and sink leaves : changes in solute transport and polypeptide composition.Plant physiology, 100 3
L. Maurousset, R. Lemoine, S. Delrot, D. Pichelin-Poitevin (1992)
Solute uptake in plasma membrane vesicles from broad bean (Vicia faba L.) leaves, 41
M. Mccauley, R. Evert (1989)
Minor Veins of the Potato (Solanum tuberosum L.) Leaf: Ultrastructure and Plasmodesmatal FrequencyBotanical Gazette, 150
Amon Amon (1949)
Copper enzymes in isolated chloroplastsPlant Physiology, 24
R. Lemoine, S. Delrot (1989)
Proton‐motive‐force‐driven sucrose uptake in sugar beet plasma membrane vesiclesFEBS Letters, 249
M. Stitt, U. Sonnewald (1995)
Regulation of metabolism in transgenic plants, 46
D. Arnon (1949)
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.Plant physiology, 24 1
E. Heinz, A. Weinstein (1984)
The overshoot phenomenon in cotransport.Biochimica et biophysica acta, 776 1
Christina Kühn, W. Quick, A. Schulz, J. Riesmeier, U. Sonnewald, W. Frommer (1996)
Companion cell‐specific inhibition of the potato sucrose transporter SUT1Plant Cell and Environment, 19
Riesmeier Riesmeier, Willmitzer Willmitzer, Frommer Frommer (1994)
Antisense repression of the sucrose transporter affects assimilate partitioning in transgenic potato plantsThe EMBO Journal, 13
ABSTRACT The sucrose proton‐cotransporter gene from potato (StSUT1) is mainly expressed in the phloem of mature, exporting leaves. To study the in vivo role of the protein, potato plants were transformed with antisense constructs of the sucrose transporter cDNA under control of the CaMV35S and the rolC promoters, respectively. Both types of transgenic plant develop symptoms characteristic of an inhibition of phloem loading. To determine the level of inhibition, immunological and transport studies were performed. Purified antibodies directed against a peptide from the central loop of SUT1 recognized a transporter with an apparent molecular mass of 47 kDa in leaf plasma membrane vesicles. Antisense repression under control of the non‐specific CaMV35S promoter led to a strong reduction in SUT1 protein, whereas no such reduction could be detected when the companion cell‐specific rolC promoter was used. Similarily. sucrose uptake in plasma membrane vesicles was reduced by 50–75% in CaMV35S but not in rolC plants. These data suggest that, unlike the rolC promoter, the sucrose transporter is expressed not only in the companion cells but also in other leaf cells. However, inhibition of the transporter by rolC‐controlled antisense repression is sufficient to impair phloem loading.
Plant Cell & Environment – Wiley
Published: Oct 1, 1996
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