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Glucocorticoid hormone binding to human adipose tissue

Glucocorticoid hormone binding to human adipose tissue Abstract. Binding of triamcinolone was examined in cytosolic preparations of human adipose tissue obtained during surgery. A saturable specific binding was found. Non‐specific binding comprised on an average 28% of total binding. The affinity and concentration of binding sites were in the same order as previously described for glucocorticoid hormone binding in other tissues and in rat adipose tissue. There was a large difference in these variables between different individuals. The binding was inhibited competitively by non‐labelled triamcinolone, promegestone, dexa‐methasone and 17‐/?‐estradiol, in that order of potency. The promegestone inhibition was effective requiring only about 25% higher concentration than triamcinolone to obtain inhibition of half the triamcinolone binding. There was more binding of triamcinolone in omental than in subcutaneous abdominal adipose tissue when expressed per unit of protein and per unit cell surface area but not when expressed per adipocyte. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Clinical Investigation Wiley

Glucocorticoid hormone binding to human adipose tissue

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References (18)

Publisher
Wiley
Copyright
Copyright © 1985 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0014-2972
eISSN
1365-2362
DOI
10.1111/j.1365-2362.1985.tb00182.x
Publisher site
See Article on Publisher Site

Abstract

Abstract. Binding of triamcinolone was examined in cytosolic preparations of human adipose tissue obtained during surgery. A saturable specific binding was found. Non‐specific binding comprised on an average 28% of total binding. The affinity and concentration of binding sites were in the same order as previously described for glucocorticoid hormone binding in other tissues and in rat adipose tissue. There was a large difference in these variables between different individuals. The binding was inhibited competitively by non‐labelled triamcinolone, promegestone, dexa‐methasone and 17‐/?‐estradiol, in that order of potency. The promegestone inhibition was effective requiring only about 25% higher concentration than triamcinolone to obtain inhibition of half the triamcinolone binding. There was more binding of triamcinolone in omental than in subcutaneous abdominal adipose tissue when expressed per unit of protein and per unit cell surface area but not when expressed per adipocyte.

Journal

European Journal of Clinical InvestigationWiley

Published: Oct 1, 1985

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