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References (30)
-
Williams Jg, Han Mk, Rafalski Ja, Tingey Sv (1993)
Genetic analysis using random amplified polymorphic DNA markers.Methods in Enzymology, 218
-
AO) 226-0.55 (AO) 226-0.57 (A0)/0.7 (P) 220-1.1 (PO) 227-0.65 (P)/0.60 (0) -
B. Bowditch, D. Albright, John Williams, Michael Braun (1993)
Use of randomly amplified polymorphic DNA markers in comparative genome studies.Methods in enzymology, 224
-
PO) 2her-0.65 (PO) 26f-0.% (PO) -
C. Heiser, D. Smith, S. Clevenger, W. Martin (1969)
The North American sunflowers (Helianthus), 22
-
DM Hillis, JP Huelsenbeck (1992)
Signal, noise, and reliability in molecular phylogenetic analysesAmerican Journal of Botany, 83
-
JJ Smith, JS Scott‐Craig, JR Leadbetter, G. Bush, DL Roberts, DW Fulbright (1994)
Characterization of random amplified polymorphic DNA (RAPD) products from Xanthomonas campestris and some comments on the use of RAPD products in phylogenetic analysisTheoretical and Applied Genetics, 3
-
P. Fritsch, LH Rieseberg (1992)
Outcrossing rates are high in androdioecious populations of the flowering plant Datisca glomerataMolecular Ecology, 359
-
0) trl-0.6 (PO) trl4.8 (P)/O.ZO (0) E17-1.6 (AO -
J. Doyle (1992)
Gene Trees and Species Trees: Molecular Systematics as One-Character TaxonomySystematic Botany, 17
-
LH Rieseberg, S. Beckstrom‐Sternberg, A. Liston, D. Arias (1991)
Phylogenetic and systematic inferences from chloroplast DNA and isozyme variation in Helianthus sectHeredity, 16
-
P. Fritsch, L. Rieseberg (1992)
High outcrossing rates maintain male and hermaphrodite individuals in populations of the flowering plant Datisca glomerataNature, 359
-
H. Hadrys, M. Balick, B. Schierwater (1992)
Applications of random amplified polymorphic DNA (RAPD) in molecular ecologyMolecular Ecology, 1
-
J. Felsenstein (1985)
CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAPEvolution, 39
-
CE Thormann, ME Ferreira, LEA Camargo, JG Tivang, TC Osborn (1994)
Comparison of RFLP and RAPD markers to estimating genetic relationships within and among cruciferous speciesNucleic Acids Research, 88
-
J. Welsh, M. McClelland (1990)
Fingerprinting genomes using PCR with arbitrary primersMethods in Enzymology, 18
-
DJ Crawford (1990)
Plant Molecular SystematicsSystematic Botany
-
L. Rieseberg (1991)
Homoploid reticulate evolution in Helianthus (Asteraceae): evidence from ribosomal genesAmerican Journal of Botany, 78
-
M. Ayliffe, G. Lawrence, J. Ellis, A. Pryor (1994)
Heteroduplex molecules formed between allelic sequences cause nonparental RAPD bands.Nucleic acids research, 22 9
-
H. Bandelt, A. Dress (1992)
Split decomposition: a new and useful approach to phylogenetic analysis of distance data.Molecular phylogenetics and evolution, 1 3
-
J. Welsh, Michael McClelland (1990)
Fingerprinting genomes using PCR with arbitrary primers.Nucleic acids research, 18 24
-
James Smith, J. Scott-Craig, J. Leadbetter, G. Bush, David Roberts, D. Fulbright (1994)
Characterization of random amplified polymorphic DNA (RAPD) products from Xanthomonas campestris and some comments on the use of RAPD products in phylogenetic analysis.Molecular phylogenetics and evolution, 3 2
-
L. Rieseberg, S. Beckstrom-Sternberg, A. Liston, D. Arias (1991)
Phylogenetic and Systematic Inferences from Chloroplast DNA and Isozyme Variation in Helianthus sect. Helianthus (Asteraceae)Systematic Botany, 16
-
BM Bowditch, DG Albright, J. Williams, MJ Braun (1993)
The use of RAPD markers in comparative genome studiesMethods in Enzymology, 224
-
L. Rieseberg, C. Fossen, A. Desrochers (1995)
Hybrid speciation accompanied by genomic reorganization in wild sunflowersNature, 375
-
D. Hillis, J. Huelsenbeck (1992)
Signal, noise, and reliability in molecular phylogenetic analyses.The Journal of heredity, 83 3
-
H. Hadrys, M. Balick, B. Schierwater (1992)
Applications of random amplified polymorhic DNA (RAPD) in molecular ecologyMemoirs of the Torrey Botanical Club, 1
-
LH Rieseberg (1991)
Homoploid reticulate evolution in Helianthus: Evidence from ribosomal genesHelianthus. Systematic Botany, 78
-
L. Rieseberg, H. Choi, Ray Chan, Chrystal Spore (1993)
Genomic map of a diploid hybrid speciesHeredity, 70
-
J. Welsh, M. McClelland (1993)
Diagnostic Molecular Microbiology
- Publisher
- Wiley
- Copyright
- Copyright © 1996 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 0962-1083
- eISSN
- 1365-294X
- DOI
- 10.1111/j.1365-294X.1996.tb00295.x
- Publisher site
- See Article on Publisher Site
Abstract
The use of RAPDs for comparative purposes relies on the assumption that similarity of fragment size is a dependable indicator of homology. To test the validity of this assumption, homology among 220 pairs of comigrating fragments from three wild sunflower species was determined. Ninety‐one per cent cross‐hybridized and/or displayed congruent restriction fragment profiles suggestive of homology. However, comparative linkage mapping data indicated that 13% of the homologous loci mapped to genomic locations that were incongruent with the majority of loci, suggestive of paralogous rather than orthologous relationships. Thus, of the 220 pairwise comparisons, only 174 (79.1%) identified loci that are useful for comparative genetic studies. These problems, as well as several other factors discussed in the text, will introduce noise into RAPD data sets and thereby reduce the probability of generating accurate estimates of genetic relationships. Recommended methods for reducing noise in RAPD data sets include increasing gel resolution and/or testing fragment homology. However, implementation of these approaches will not eliminate all uncertainties, and it is also recommended that RAPD data sets be tested for structure and reliability.
Journal
Molecular Ecology – Wiley
Published: Feb 1, 1996
Keywords: ; ; ; ; ; ;