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Photosynthesis nuclear genes generally lack TATA‐boxes: a tobacco photosystem I gene responds to light through an initiator

Photosynthesis nuclear genes generally lack TATA‐boxes: a tobacco photosystem I gene responds to... The promoter architecture of the nuclear‐encoded photosystem I genes was studied using a tobacco gene, psaDb, as a model case. Linker scanning mutations revealed that the psaDb promoter does not have a TATA box. Instead, pyrimidine‐rich Initiator (Inr) elements that overlap the transcription start sites are essential for light‐responsive transcription of this gene. When the psaDb promoter was mutated to have a TATA‐box but no Inr, light‐responsive transcription was impaired, indicating that the regulatory system of this gene prefers Inr to a TATA box. As very little is known about plant TATA‐less promoters, we subsequently examined whether this promoter architecture is unique to psaDb. Computer analysis of 232 plant promoters revealed surprising features; the majority of photosynthesis nuclear genes lacked TATA boxes, although the frequency of the TATA‐less promoters in non‐photosynthesis genes was less than 10%. These results strongly suggest that TATA‐independent transcription mechanisms play important roles in the regulated expression of photosynthesis nuclear genes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Plant Journal Wiley

Photosynthesis nuclear genes generally lack TATA‐boxes: a tobacco photosystem I gene responds to light through an initiator

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References (61)

Publisher
Wiley
Copyright
Copyright © 2002 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0960-7412
eISSN
1365-313X
DOI
10.1046/j.0960-7412.2001.01188.x
Publisher site
See Article on Publisher Site

Abstract

The promoter architecture of the nuclear‐encoded photosystem I genes was studied using a tobacco gene, psaDb, as a model case. Linker scanning mutations revealed that the psaDb promoter does not have a TATA box. Instead, pyrimidine‐rich Initiator (Inr) elements that overlap the transcription start sites are essential for light‐responsive transcription of this gene. When the psaDb promoter was mutated to have a TATA‐box but no Inr, light‐responsive transcription was impaired, indicating that the regulatory system of this gene prefers Inr to a TATA box. As very little is known about plant TATA‐less promoters, we subsequently examined whether this promoter architecture is unique to psaDb. Computer analysis of 232 plant promoters revealed surprising features; the majority of photosynthesis nuclear genes lacked TATA boxes, although the frequency of the TATA‐less promoters in non‐photosynthesis genes was less than 10%. These results strongly suggest that TATA‐independent transcription mechanisms play important roles in the regulated expression of photosynthesis nuclear genes.

Journal

The Plant Journal – Wiley

Published: Jan 1, 2002

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